KEGG   ENZYME: 1.14.14.132
Entry
EC 1.14.14.132              Enzyme                                 
Name
(-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase;
CYP82D61 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(-)-deoxypodophyllotoxin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
(-)-4'-demethyldeoxypodophyllotoxin + [reduced NADPH---hemoprotein reductase] + O2 = (-)-4'-demethylepipodophyllotoxin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11212]
Reaction(KEGG)
R11212
Substrate
(-)-4'-demethyldeoxypodophyllotoxin [CPD:C10552];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-4'-demethylepipodophyllotoxin [CPD:C21185];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein characterized from the plant Sinopodophyllum hexandrum. The enzyme produces the direct precursor to etoposide, a potent anticancer drug. It can also act on (-)-deoxypodophyllotoxin with lower efficiency.
History
EC 1.14.14.132 created 2016 as EC 1.14.13.214, transferred 2018 to EC 1.14.14.132
Orthology
K21206  (-)-4'-demethyl-deoxypodophyllotoxin 4-hydroxylase
Reference
1  [PMID:26359402]
  Authors
Lau W, Sattely ES
  Title
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone.
  Journal
Science 349:1224-8 (2015)
DOI:10.1126/science.aac7202
  Sequence
[ag:ALG05144]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.132
IUBMB Enzyme Nomenclature: 1.14.14.132
ExPASy - ENZYME nomenclature database: 1.14.14.132
BRENDA, the Enzyme Database: 1.14.14.132

  All links  
Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (5)   
   InterPro (5)   
All databases (17)   

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