KEGG ENZYME: 1.14.14.133

ENZYME: 1.14.14.133

Entry

EC 1.14.14.133              Enzyme

Name

1,8-cineole 2-endo-monooxygenase;
P450cin;
CYP176A;
CYP176A1

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

1,8-cineole,[reduced flavodoxin]:oxygen oxidoreductase (2-endo-hydroxylating)

Reaction(IUBMB)

1,8-cineole + [reduced flavodoxin] + O2 = 2-endo-hydroxy-1,8-cineole + [oxidized flavodoxin] + H2O [RN:R08784]

Reaction(KEGG)

R08784

Substrate

1,8-cineole [CPD:C09844];
reduced flavodoxin [CPD:C02745];
O2 [CPD:C00007]

Product

2-endo-hydroxy-1,8-cineole [CPD:C03092];
oxidized flavodoxin [CPD:C02869];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein that uses a flavodoxin-like redox partner to reduce the heme iron. Isolated from the bacterium Citrobacter braakii, which can use 1,8-cineole as the sole source of carbon.

History

EC 1.14.14.133 created 2012 as EC 1.14.13.156, transferred 2018 to EC 1.14.14.133

Pathway

ec00902

Monoterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K21120

1,8-cineole 2-endo-monooxygenase

Genes