Entry
Name
beta-amyrin 11-oxidase;
CYP88D6
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
beta-amyrin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
beta-amyrin + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = 11-oxo-beta-amyrin + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:
R09817 ];
(1a) beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 11alpha-hydroxy-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R09815 ];
(1b) 11alpha-hydroxy-beta-amyrin + [reduced NADPH---hemoprotein reductase] + O2 = 11-oxo-beta-amyrin + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:
R09816 ]
Reaction(KEGG)
Substrate
beta-amyrin [CPD:
C08616 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ];
11alpha-hydroxy-beta-amyrin [CPD:
C19942 ]
Product
11-oxo-beta-amyrin [CPD:
C19943 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ];
11alpha-hydroxy-beta-amyrin [CPD:
C19942 ]
Comment
A cytochrome P-450 (heme-thiolate) protein from the plant Glycyrrhiza uralensis (Chinese licorice) that participates in the glycyrrhizin biosynthesis pathway. The enzyme is also able to oxidize 30-hydroxy-beta-amyrin to 11alpha,30-dihydroxy-beta-amyrin but this is not thought to be part of glycyrrhizin biosynthesis.
History
EC 1.14.14.152 created 2011 as EC 1.14.13.134, transferred 2018 to EC 1.14.14.152
Orthology
Genes
CCAJ : 109791034 109814269
APRC : 113850515 113850517 113850522 113851591 113858948 113863306
MTR : 11434382 120575790 120577696 120577701 25500330 25501850
TPRA : 123883543 123883544 123883548 123886813 123889857 123902345
CAM : 101495501 101495829 101496150 101496479
PSAT : 127092685 127092902 127097636 127107170
VVO : 131595905 131595906 131595907 131600277 131602614 131606077
LJA : Lj3g3v0730100.1(Lj3g3v0730100.1) Lj3g3v1983610.1(Lj3g3v1983610.1) Lj3g3v1983620.1(Lj3g3v1983620.1) Lj3g3v1983620.2(Lj3g3v1983620.2) Lj3g3v1993700.1(Lj3g3v1993700.1) Lj3g3v1993710.1(Lj3g3v1993710.1)
QSA : O6P43_017453 O6P43_021806
» show all
Taxonomy
Reference
Authors
Seki H, Ohyama K, Sawai S, Mizutani M, Ohnishi T, Sudo H, Akashi T, Aoki T, Saito K, Muranaka T
Title
Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin.
Journal
Sequence
Other DBs