KEGG ENZYME: 1.14.14.158

ENZYME: 1.14.14.158

Entry

EC 1.14.14.158              Enzyme

Name

carotenoid epsilon hydroxylase;
CYP97C1;
LUT1;
CYP97C;
carotene epsilon-monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

alpha-carotene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)

Reaction(IUBMB)

(1) alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2 = alpha-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07850];
(2) zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 = lutein + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R07531]

Reaction(KEGG)

R07531 R07850

Substrate

alpha-carotene [CPD:C05433];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007];
zeinoxanthin [CPD:C08590]

Product

alpha-cryptoxanthin [CPD:C15981];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001];
lutein [CPD:C08601]

Comment

A cytochrome P-450 (heme-thiolate) protein.

History

EC 1.14.14.158 created 2011 as EC 1.14.99.45, transferred 2018 to EC 1.14.14.158

Pathway

ec00906

Carotenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09837

carotenoid epsilon hydroxylase

Genes

ATH:	AT3G53130(LUT1)

ALY:

9313971

CRB:

17887227

CSAT:

104711869 104781166 104791575

EUS:	EUTSA_v10010267mg EUTSA_v10023482mg

BRP:

103850280

BNA:	106352760 106452442

BOE:

106300296

RSZ:

108814454

THJ:

104818583

CPAP:

110822860

CIT:

102625870

CIC:	CICLE_v10011420mg

PVY:

116143852

MINC:

123228821

TCC:

18598092

GRA:

105761792

GHI:	121209533 121223568

GAB:

108470606

HSYR:

120158085

DZI:

111296450

GMX:	100797221 100803324

GSJ:	114372632 114382715

PVU:	PHAVU_007G2265001

VRA:

106772401

VAR:

108326589

VUN:

114192824

VUM:

124828972

CCAJ:

109818061

APRC:

113872166

MTR:

11429551

TPRA:

123907402

CAM:	101497194 101497524

PSAT:

127093315

VVO:

131644652

LJA:	Lj5g3v0681210.1(Lj5g3v0681210.1)

ADU:

107476248

AIP:

107626444

AHF:	112728911 112759486

LANG:

109360988

PCIN:

129304715

QSA:	O6P43_006591

FVE:

101308016

RCN:

112191404

PPER:

18771385

PMUM:

103339783

PAVI:

110747444

PDUL:

117635766

MDM:

103454736

MSYL:

126599597

PXB:	103933149 103964559

ZJU:

107432039

MNT:

21405423

CSAV:

115723298

CSV:

101204961

CMO:

103500849

BHJ:

120073806

MCHA:

111013687

CMAX:

111471281

CMOS:

111463211

CPEP:

111782219

RCU:

8283943

JCU:

105642313

HBR:

110662670

MESC:

110620012

POP:

18100110

PEU:

105121750

PALZ:

118048373

JRE:	108985270 118347955

CILL:

122275262

CAVE:

132191717

QSU:

111984908

QLO:

115953099

TWL:

119985060

VVI:

100243621

VRI:	117920315 117920676

SLY:	100322877(CYP97C11)

SPEN:

107002514

SOT:

102591274

SSTN:

125865574

SDUL:

129875508

CANN:

107843608

LBB:

132607146

NTA:	107761917 107797056

NSY:

104210223

NTO:

104114442

NAU:

109237187

INI:

109181096

ITR:

116006108

SIND:

105174233

OEU:

111404128

EGT:

105952850

SSPL:

121798854

SMIL:

130997867

SHIS:

125199993 125217902

APAN:

127251569

HAN:

110882135

ECAD:

122581976

LSV:

111913167

CCAV:

112519301

DCR:

108220021

CSIN:

114262489 114262504

RVL:

131324690

AEW:

130752467

BVG:

104898583

SOE:

110788578

CQI:	110683094 110738483

ATRI:

130827141

MOF:

131167963

NNU:

104586192

MING:

122087098

TSS:

122655775

PSOM:

113313399

OSA:

4349290

DOSA:

Os10t0546600-01(Os10g0546600)

OBR:

102700091

OGL:

127753455

BDI:

100835344

ATS:

109740072

TDC:	119274766 119338491

TAES:

123051536 123127494 123181586

LPER:

127308289

LRD:

124706203

SBI:

8058776

ZMA:

103637620

SITA:

101758095

SVS:

117836012

PVIR:

120651787 120690677

PHAI:

112876006

PDA:

103719551

EGU:

105034264

MUS:

103970366

ZOF:	122003038 122007293

DCT:

110099518

PEQ:

110030448

AOF:

109825600

MSIN:

131224791

NCOL:

116253613

ATR:

18424541

SMO:	SELMODRAFT_186370(CYP97C15) SELMODRAFT_187123

PPP:

112283348

VCN:	VOLCADRAFT_83281

MNG:

MNEG_1212

CSL:	COCSUDRAFT_63212

CVR:	CHLNCDRAFT_51247

APRO:

F751_6907

OLU:	OSTLU_33533

OTA:	OT_ostta09g02540

BPG:	Bathy10g01980

MIS:	MICPUN_95887

MPP:	MICPUCDRAFT_32152(JGI:MicpuC2_32152)

» show all

Reference

1 [PMID:8837513]

Authors

Pogson B, McDonald KA, Truong M, Britton G, DellaPenna D

Title

Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants.

Journal

Plant Cell 8:1627-39 (1996)
DOI:10.1105/tpc.8.9.1627

Reference

2 [PMID:14709673]

Authors

Tian L, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D.

Title

The Arabidopsis LUT1 locus encodes a member of the cytochrome p450 family that is required for carotenoid epsilon-ring hydroxylation activity.

Journal

Proc Natl Acad Sci U S A 101:402-7 (2004)
DOI:10.1073/pnas.2237237100

Sequence

[ath:AT3G53130]

Reference

3 [PMID:21450689]

Authors

Stigliani AL, Giorio G, D'Ambrosio C

Title

Characterization of P450 carotenoid beta- and epsilon-hydroxylases of tomato and transcriptional regulation of xanthophyll biosynthesis in root, leaf, petal and fruit.

Journal

Plant Cell Physiol 52:851-65 (2011)
DOI:10.1093/pcp/pcr037

Sequence

[sly:100322877]

Reference

4 [PMID:26030746]

Authors

Chang S, Berman J, Sheng Y, Wang Y, Capell T, Shi L, Ni X, Sandmann G, Christou P, Zhu C

Title

Cloning and Functional Characterization of the Maize (Zea mays L.) Carotenoid Epsilon Hydroxylase Gene.

Journal

PLoS One 10:e0128758 (2015)
DOI:10.1371/journal.pone.0128758