germacrene A acid 8beta-hydroxylase;
HaG8H;
CYP71BL1;
CYP71BL6
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
A cytochrome P-450 (heme-thiolate) protein from the plant Helianthus annuus (common sunflower). The cyclisation of 8beta-hydroxygermacra-1(10),4,11(13)-triene-12-oate to inunolide (12,8beta) does not seem to occur spontaneously. The enzyme from Inula hupehensis also forms some 8alpha-hydroxygermacra-1(10),4,11(13)-triene-12-oate, which spontaneously cyclises to 8-epi-inunolide (12,8alpha) (cf. EC 1.14.14.170 8-epi-inunolide synthase).
Gou J, Hao F, Huang C, Kwon M, Chen F, Li C, Liu C, Ro DK, Tang H, Zhang Y
Title
Discovery of a non-stereoselective cytochrome P450 catalyzing either 8alpha- or 8beta-hydroxylation of germacrene A acid from the Chinese medicinal plant, Inula hupehensis.