KEGG ENZYME: 1.14.14.179

ENZYME: 1.14.14.179

Entry

EC 1.14.14.179              Enzyme

Name

brassinosteroid 6-oxygenase;
CYP85A1 (gene name);
CYP85A2 (gene name);
brassinosteroid 6-oxidase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

6-deoxocastasterone,NADPH---hemoprotein reductase:oxygen 6-oxidoreductase (castasterone-forming)

Reaction(IUBMB)

6-deoxocastasterone + 2 O2 + 2 [reduced NADPH---hemoprotein reductase] = castasterone + 3 H2O + 2 [oxidized NADPH---hemoprotein reductase] (overall reaction) [RN:R10669];
(1a) 6-deoxocastasterone + O2 + [reduced NADPH---hemoprotein reductase] = 6alpha-hydroxy-6-deoxocastasterone + H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07450];
(1b) 6alpha-hydroxy-6-deoxocastasterone + O2 + [reduced NADPH---hemoprotein reductase] = castasterone + 2 H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07451]

Reaction(KEGG)

R07450 R07451 R10669;
(other) R07455 R07457 R07458

Substrate

6-deoxocastasterone [CPD:C15802];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
6alpha-hydroxy-6-deoxocastasterone

Product

castasterone [CPD:C15794];
H2O [CPD:C00001];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
6alpha-hydroxy-6-deoxocastasterone

Comment

This cytochrome P-450 (heme thiolate) plant enzyme catalyses the C-6 hydoxylation of several brassinosteroid biosynthesis intermediates, and the further oxidation of the hydroxyl group to an oxo group. Substrates include 6-deoxocastasterone, 6-deoxotyphasterol, 3-dehydro-6-deoxoteasterone, and 6-deoxoteasterone. The CYP85A2 isozyme of Arabidopsis thaliana (but not the CYP85A1 isozyme) also catalyses the activity of EC 1.14.14.180, brassinolide synthase.

History

EC 1.14.14.179 created 2022

Pathway

ec00905

Brassinosteroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09590

brassinosteroid 6-oxygenase

K12640

brassinosteroid 6-oxygenase / brassinolide synthase

K25959

brassinosteroid 6-oxygenase / brassinolide synthase

Genes

ATH:	AT3G30180(BR6OX2) AT5G38970(BR6OX1)

ALY:	9306879 9311596

CRB:	17880458 17885676

CSAT:

104710811 104719559 104724260 104732939 104780197 104790595

EUS:	EUTSA_v10004156mg EUTSA_v10027742mg

BRP:	103837220 103854484 103864167 103875063

BNA:	106349108 106363995 106397123 106400040 106410433 106449738 125575048 125599608

BOE:	106301844 106316572 106339725

RSZ:	108807315 108825972 108837548 108849348

THJ:	104805522 104822777 104823884

CPAP:

110820131 110823995

CIT:	102613870 102627864

CIC:	CICLE_v10011662mg CICLE_v10031449mg

PVY:	116109710 116132910

MINC:

123198264 123213958 123216775 123226215

TCC:	18601740 18610219

GRA:	105781807 105789387 105801672

GHI:	107894854 107910165 107919617 107954898 121219372 121232120

GAB:	108453607 108463311 108482799

HSYR:

120113986 120127048 120130453 120130527 120152241 120153181 120153818 120164120 120170916 120171060

DZI:	111283830 111306268 111313564 111317590

EGR:	104419439 104422041 104422043 104422045

GMX:	100779967 100785414 100805958 100818004 100818290

GSJ:	114372680 114381879 114395015 114399240 114423881

PVU:	PHAVU_003G040300g PHAVU_004G041700g PHAVU_008G028800g

VRA:	106753400 106758156 106779292

VAR:	108326910 108335521 108344744

VUN:	114166182 114181354 114183716

VUM:	124819671 124832523 124839185

CCAJ:

109796264 109797613 109813276 109818989

APRC:

113861763 113867653 113869100 113869572

MTR:	11422367 25495426 25497603

TPRA:

123893063 123908660

CAM:	101509866 101510185 101510551

PSAT:

127115423 127115432 127120958 127128705

VVO:	131598744 131598753 131628644 131629940 131630104

LJA:	Lj0g3v0092539.1(Lj0g3v0092539.1) Lj0g3v0228369.1(Lj0g3v0228369.1) Lj2g3v1241200.1(Lj2g3v1241200.1) Lj2g3v1241200.2(Lj2g3v1241200.2) Lj2g3v1241200.3(Lj2g3v1241200.3) Lj2g3v1241200.4(Lj2g3v1241200.4)

ADU:	107471377 107486576

AIP:	107623811 107637361

AHF:	112716544 112716545 112744628 112784627 112784628 112797930

LANG:

109330371 109335133 109347392 109359433 109359685 109360156

PCIN:

129289879 129306523 129311025 129311028

QSA:	O6P43_004380 O6P43_009190 O6P43_032891

FVE:

101313910

RCN:

112190949

PPER:

18782277

PMUM:

103329572

PAVI:

110759051

PDUL:

117623030

MDM:	103404651 103442442

MSYL:

126583959 126612490

PXB:	103926586 103962743

ZJU:

125421869

MNT:	21401153 21409111

CSAV:

115698729

CSV:	101204494 101206858

CMO:	103495886 103498545

BHJ:

120079745

MCHA:

111006661

CMAX:

111487713 111489110

CMOS:

111445417 111455792

CPEP:

111788371 111808466

RCU:

8277307

JCU:	105630569 105646746

HBR:	110648160 110655288 110672384 110672390

MESC:

110609099 110615056 110619853

POP:	18107339 7468141 7480593

PEU:	105113937 105119329 105123253

PALZ:

118042705 118043350 118055061

JRE:	108987930 108989489

CILL:

122303021 122306104

CAVE:

132179667 132181229

QSU:	111985795 112004758 112004759 112006726

QLO:	115976259 115981444 115981445

TWL:	119993370 120011335 120017232

VVI:	100232983(BR6OX1) 100257818 100262943

VRI:	117923784 117925175 117929660

SLY:	100037489(CYP85A1) 100136888(CYP85A3)

SPEN:

107009077 107010718

SOT:	102599687 102603315

SSTN:

125848344 125849224

SDUL:

129874522 129875118

CANN:

107860427 107861006

LBB:

132621167

NTA:	107760973 107776096 107780292 107789559

NSY:	104211653 104214321

NTO:	104092995 104103228

NAU:	109212730 109222927

INI:	109153620 109173064 109180625 109189067

ITR:	115999955 116013189 116013963 116019775

SIND:

105156751 105177542

OEU:	111374568 111381944 111409964

EGT:	105952596 105971548

SSPL:

121754265 121755396 121767430 121770076

SMIL:

130987156 130989766

SHIS:

125208620 125220261 125224420

APAN:

127244512 127258238 127263988

HAN:	110911035 110921448

ECAD:

122579005 122589730

LSV:

111876869

CCAV:

112513235 112515661

DCR:	108193941 108194607 108213230

CSIN:

114256381 114306437

RVL:	131301037 131304513 131336204

AEW:	130751052 130768177 130778234 130778892

BVG:

104906946

SOE:

110793171

CQI:	110703563 110705784

ATRI:

130802184 130803910

MOF:	131154317 131160507

NNU:	104597895 104599689 104609612

MING:

122075756 122078986

TSS:	122655820 122663736

PSOM:

113275767 113281843

OSA:

4333399

DOSA:

Os03t0602300-01(Os03g0602300)

OBR:

102700319

OGL:

127767630

BDI:

100837653

ATS:	109777766 109777767 109777768 109777769 109777770

TDC:	119352698 119352699 119358574 119358575 119360155 119361707 119361709 119366782 119366783

TAES:

123038724 123039915 123043102 123043103 123048178 123048179 123050998 123051000 123051001 123184190 123186954 123186955 123186956

TUA:	125534977 125540566 125540569 125540570

LPER:

127333421

LRD:

124674413

SBI:

8062533

ZMA:

100193331

SITA:

101770408

SVS:

117838234

PVIR:

120650403 120692586

PHAI:

112875680

PDA:	103696100 103704209 103704493 103722877

EGU:	105046675 105056073 105057148 105058109

MUS:	103975616 103982137 103986539 103996475

ZOF:	121975689 122012212 122017716 122034502 122039599 122049062

DCT:	110095594 110112633

PEQ:	110030284 110033615 110034826 110039402

AOF:	109822473 109825906 109845117 109846361

MSIN:

131219410 131229521

NCOL:

116258091 116258773

ATR:	18446218 18446219

PPP:

112292180

» show all

Reference

1 [PMID:11402205]

Authors

Shimada Y, Fujioka S, Miyauchi N, Kushiro M, Takatsuto S, Nomura T, Yokota T, Kamiya Y, Bishop GJ, Yoshida S

Title

Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis.

Journal

Plant Physiol 126:770-9 (2001)
DOI:10.1104/pp.126.2.770

Sequence

[ath:AT5G38970] [sly:100037489]

Reference

2 [PMID:21364326]

Authors

Perez-Espana VH, Sanchez-Leon N, Vielle-Calzada JP.

Title

CYP85A1 is required for the initiation of female gametogenesis in Arabidopsis thaliana.

Journal

Plant Signal Behav 6:321-6 (2011)
DOI:10.4161/psb.6.3.13206

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.179

IUBMB Enzyme Nomenclature:

1.14.14.179

ExPASy - ENZYME nomenclature database:

1.14.14.179

BRENDA, the Enzyme Database:

1.14.14.179

DBGET integrated database retrieval system