KEGG ENZYME: 1.14.14.180

ENZYME: 1.14.14.180

Entry

EC 1.14.14.180              Enzyme

Name

brassinolide synthase;
CYP85A2 (gene name);
CYP85A3 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

castasterone,NADPH---hemoprotein reductase:oxygen oxidoreductase (lactonizing, brassinolide-forming)

Reaction(IUBMB)

castasterone + O2 + [reduced NADPH---hemoprotein reductase] = brassinolide + 2 H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07471]

Reaction(KEGG)

R07471;
(other) R08840 R08841

Substrate

castasterone [CPD:C15794];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]

Product

brassinolide [CPD:C08814];
H2O [CPD:C00001];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161]

Comment

This cytochrome P-450 (heme thiolate) plant enzyme catalyses the lactonization of several brassinosteroids, including castasterone, teasterone, and typhasterol. The CYP85A2 enzyme of Arabidopsis thaliana also catalyses the activity of EC 1.14.14.179, brassinosteroid 6-oxygenase.

History

EC 1.14.14.180 created 2022

Pathway

ec00905

Brassinosteroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12640

brassinosteroid 6-oxygenase / brassinolide synthase

K25959

brassinosteroid 6-oxygenase / brassinolide synthase

Genes

ATH:	AT3G30180(BR6OX2)

ALY:

9311596

CRB:

17885676

CSAT:

104710811 104780197 104790595

EUS:	EUTSA_v10004156mg

BRP:	103837220 103854484 103875063

BNA:	106349108 106363995 106397123 106400040 106410433

BOE:	106301844 106316572

RSZ:	108807315 108825972 108849348

THJ:	104805522 104822777

SLY:	100136888(CYP85A3)

SPEN:

107010718

SOT:

102599687

SSTN:

125848344

SDUL:

129874522

CANN:

107861006

» show all

Reference

1 [PMID:15710611]

Authors

Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S

Title

The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis.

Journal

J Biol Chem 280:17873-9 (2005)
DOI:10.1074/jbc.M414592200

Sequence

[sly:100136888] [ath:AT3G30180]

Reference

2 [PMID:16024588]

Authors

Kim TW, Hwang JY, Kim YS, Joo SH, Chang SC, Lee JS, Takatsuto S, Kim SK.

Title

Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis.

Journal

Plant Cell 17:2397-412 (2005)
DOI:10.1105/tpc.105.033738

Sequence

[ath:AT3G30180]

Reference

3 [PMID:18685225]

Authors

Katsumata T, Hasegawa A, Fujiwara T, Komatsu T, Notomi M, Abe H, Natsume M, Kawaide H

Title

Arabidopsis CYP85A2 catalyzes lactonization reactions in the biosynthesis of 2-deoxy-7-oxalactone brassinosteroids.

Journal

Biosci Biotechnol Biochem 72:2110-7 (2008)
DOI:10.1271/bbb.80192

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.180

IUBMB Enzyme Nomenclature:

1.14.14.180

ExPASy - ENZYME nomenclature database:

1.14.14.180

BRENDA, the Enzyme Database:

1.14.14.180

DBGET integrated database retrieval system