KEGG   ENZYME: 1.14.14.182
Entry
EC 1.14.14.182              Enzyme                                 
Name
taxoid 7beta-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
taxusin, [reduced NADPH---hemoprotein reductase]:oxygen 7-oxidoreductase
Reaction(IUBMB)
(1) taxusin + [reduced NADPH---hemoprotein reductase] + O2 = 7beta-hydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R09868];
(2) 2alpha-hydroxytaxusin + [reduced NADPH---hemoprotein reductase] + O2 = 2alpha,7beta-dihydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13018]
Reaction(KEGG)
R09868 R13018
Substrate
taxusin [CPD:C20152];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
2alpha-hydroxytaxusin [CPD:C22520]
Product
7beta-hydroxytaxusin [CPD:C20153];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
2alpha,7beta-dihydroxytaxusin [CPD:C22521]
Comment
A cytochrome P-450 (heme-thiolate) protein from the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
History
EC 1.14.14.182 created 2012 as EC 1.14.13.147, transferred 2022 to EC 1.14.14.182
Orthology
K21720  taxoid 7beta-hydroxylase
Reference
1  [PMID:15157877]
  Authors
Chau M, Jennewein S, Walker K, Croteau R
  Title
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7 beta-hydroxylase.
  Journal
Chem Biol 11:663-72 (2004)
DOI:10.1016/j.chembiol.2004.02.025
  Sequence
[ag:AAQ75553]
Reference
2  [PMID:15178487]
  Authors
Chau M, Croteau R.
  Title
Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis.
  Journal
Arch Biochem Biophys 427:48-57 (2004)
DOI:10.1016/j.abb.2004.04.016
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.182
IUBMB Enzyme Nomenclature: 1.14.14.182
ExPASy - ENZYME nomenclature database: 1.14.14.182
BRENDA, the Enzyme Database: 1.14.14.182

  All links  
Chemical substance (8)   
   KEGG COMPOUND (8)   
Chemical reaction (3)   
   KEGG REACTION (2)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (19)   

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