Entry
Name
taxoid 2alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
taxusin, [reduced NADPH---hemoprotein reductase]:oxygen 2-oxidoreductase
Reaction(IUBMB)
(1) taxusin + [reduced NADPH---hemoprotein reductase] + O2 = 2alpha-hydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R13019 ];
(2) 7beta-hydroxytaxusin + [reduced NADPH---hemoprotein reductase] + O2 = 2alpha,7beta-dihydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R13020 ]
Reaction(KEGG)
Substrate
taxusin [CPD:
C20152 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ];
7beta-hydroxytaxusin [CPD:
C20153 ]
Product
2alpha-hydroxytaxusin [CPD:
C22520 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ];
2alpha,7beta-dihydroxytaxusin [CPD:
C22521 ]
Comment
A cytochrome P-450 (heme-thiolate) protein from the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
History
EC 1.14.14.183 created 2022
Orthology
K25953 taxoid 2alpha-hydroxylase
Reference
Authors
Chau M, Jennewein S, Walker K, Croteau R
Title
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7 beta-hydroxylase.
Journal
Reference
Authors
Chau M, Croteau R.
Title
Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis.
Journal
Sequence
Other DBs