KEGG   ENZYME: 1.14.14.183
Entry
EC 1.14.14.183              Enzyme                                 
Name
taxoid 2alpha-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
taxusin, [reduced NADPH---hemoprotein reductase]:oxygen 2-oxidoreductase
Reaction(IUBMB)
(1) taxusin + [reduced NADPH---hemoprotein reductase] + O2 = 2alpha-hydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13019];
(2) 7beta-hydroxytaxusin + [reduced NADPH---hemoprotein reductase] + O2 = 2alpha,7beta-dihydroxytaxusin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13020]
Reaction(KEGG)
R13019 R13020
Substrate
taxusin [CPD:C20152];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
7beta-hydroxytaxusin [CPD:C20153]
Product
2alpha-hydroxytaxusin [CPD:C22520];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
2alpha,7beta-dihydroxytaxusin [CPD:C22521]
Comment
A cytochrome P-450 (heme-thiolate) protein from the yew tree Taxus cuspidata. Does not act on earlier intermediates in taxol biosynthesis.
History
EC 1.14.14.183 created 2022
Orthology
K25953  taxoid 2alpha-hydroxylase
Reference
1  [PMID:15157877]
  Authors
Chau M, Jennewein S, Walker K, Croteau R
  Title
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7 beta-hydroxylase.
  Journal
Chem Biol 11:663-72 (2004)
DOI:10.1016/j.chembiol.2004.02.025
Reference
2  [PMID:15178487]
  Authors
Chau M, Croteau R.
  Title
Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis.
  Journal
Arch Biochem Biophys 427:48-57 (2004)
DOI:10.1016/j.abb.2004.04.016
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.183
IUBMB Enzyme Nomenclature: 1.14.14.183
ExPASy - ENZYME nomenclature database: 1.14.14.183
BRENDA, the Enzyme Database: 1.14.14.183

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