KEGG ENZYME: 1.14.14.48

ENZYME: 1.14.14.48

Entry

EC 1.14.14.48               Enzyme

Name

jasmonoyl-L-amino acid 12-hydroxylase;
CYP94B1 (gene name);
CYP94B3 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

jasmonoyl-L-amino acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)

Reaction(IUBMB)

a jasmonoyl-L-amino acid + [reduced NADPH---hemoprotein reductase] + O2 = a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11806]

Reaction(KEGG)

R11806

Substrate

jasmonoyl-L-amino acid [CPD:C21509];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

12-hydroxyjasmonoyl-L-amino acid [CPD:C21696];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P450 (heme thiolate) enzyme found in plants. The enzyme acts on jasmonoyl-L-amino acid conjugates, catalysing the hydroxylation of the C-12 position of jasmonic acid. While the best studied substrate is (+)-7-epi-jasmonoyl-L-isoleucine, the enzyme was shown to be active with jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and is likely to be active with other jasmonoyl-amino acid conjugates.

History

EC 1.14.14.48 created 2017

Orthology

K20665

jasmonoyl-L-amino acid 12-hydroxylase

Genes

ATH:	AT3G01900(CYP94B2) AT3G48520(CYP94B3) AT5G63450(CYP94B1)

ALY:	9300923 9311979 9318229

CRB:	17874658 17885442 17892234

CSAT:

104711346 104720538 104726960 104741179 104762484 104763703 104773500 104780667 104791069

EUS:	EUTSA_v10005707mg EUTSA_v10011125mg

BRP:	103837470 103848967 103855044 103873137 103873527 117125840

BNA:	106351873 106364122 106382901 106405321 106409295 106428634 106430785 106443168 111208658 111213368 125575957 125583567 125598047

BOE:	106294052 106308148 106316769 106325388 106333823

RSZ:	108809281 108809304 108825449 108825450 108834123 108856870 108859677 130512374

THJ:	104818188 104821513 104822524

CPAP:

110807430 110820488

CIT:	102610480 102629732

CIC:	CICLE_v10017920mg CICLE_v10019793mg

PVY:	116105682 116110514 116130733

MINC:

123195622 123211695 123223852

TCC:	108661791 18605092

GRA:	105781059 105788686 105798446 105802102

GHI:	107888050 107892164 107899717 107912087 107923036 107950185 107960454

GAB:	108454222 108459965 108466763 108477615

HSYR:

120138496 120200230 120214354

DZI:	111290941 111292713 111303810 111315893

EGR:	104416900 104416919 104419143 104422706 104434222 104434223 104434224

GMX:	100786130 100797785 100800427 100810294 100815295

GSJ:	114371922 114390206 114399625 114406286 114406288

PVU:	PHAVU_001G094200g PHAVU_010G074400g

VRA:	106753837 106773363

VAR:	108328352 108346692

VUN:	114165352 114171458 114180532

VUM:	124827085 124838749

CCAJ:

109809705 109814574 109814578

APRC:

113858257

MTR:	11443027 25497835

TPRA:

123891388 123908165

CAM:	101507161 101511677

PSAT:

127083433 127088038 127128150

VVO:	131638018 131638713 131655949 131655951 131655955

LJA:	Lj0g3v0055949.1(Lj0g3v0055949.1)

ADU:

107494813

AIP:

107605023

AHF:	112697200 112755098

LANG:

109344366

PCIN:

129305298 129306077 129319599

QSA:	O6P43_002551 O6P43_004817

FVE:	101291070 105351203

RCN:	112178560 112188217

PPER:

18776547 18783641

PMUM:

103330340 103338054

PAVI:

110763155 110764345

PDUL:

117621128 117627092

MDM:	103404195 103409661 114826857

MSYL:

126583781 126600491 126609913 126627217

PXB:	103950019 103958049 103960885 103964059

ZJU:	107418019 107427926

MNT:	21388965 21394049

CSAV:

115698768 115708787

CSV:	101202989 101207598

CMO:	103491786 103502328

BHJ:	120071683 120084009

MCHA:

111007253

CMAX:

111466028 111481487 111485059

CMOS:

111429724 111435168 111440917 111441980

CPEP:

111780340 111790737 111792218 111793378

RCU:	8269245 8271459

JCU:	105634463 105640206

HBR:	110665259 110672803

MESC:

110605550 110616961 110631223

POP:	7484471 7495780

PEU:	105110545 105119630 105139432

PALZ:

118044706 118063345

JRE:	108994307 108994389 109013637 109021892

CILL:

122280892 122289334 122302742 122307368

CAVE:

132175872 132182457

QSU:	112005217 112012674

QLO:	115964193 115976448

TWL:	120003769 120009844

VVI:	100245827 100251407

VRI:	117905025 117930395

SLY:	101262587 101263302 101263399 101263598

SPEN:

107008968 107013853 107013910 107013946

SOT:	102585085 102585163 102585498 102586169

SSTN:

125849018 125859584 125860926 125860927

SDUL:

129874172 129895397 129895546 129895618

CANN:

107860765 107862677 107862678 107866012

LBB:	132622358 132632516 132632517

NTA:	107766030 107781029 107791570 107796932 107801204 107814708 107827898

NSY:	104214653 104217827 104232917 104241490

NTO:	104096380 104099650 104101764 104112197

NAU:	109223615 109239328 109239329 109244265

INI:

109171722

ITR:

116000316

SIND:

105166192 105166954

OEU:	111385797 111390746

EGT:	105951673 105964231

SSPL:

121747247 121751253 121761150 121763652

SMIL:

130987799 131016086

SHIS:

125187069

APAN:

127241267 127258176

HAN:	110868163 110911674 110925776

ECAD:

122580456 122608462 122609539

LSV:	111877549 111920155

CCAV:

112511704 112513077

DCR:	108194102 108207998

CSIN:

114256410

RVL:	131304238 131335155

AEW:	130768435 130796229

BVG:

104893484

SOE:

110787294

CQI:	110724296 110724297 110739743

ATRI:

130797470

MOF:	131160555 131168261

NNU:	104592904 104592906

MING:

122077558

TSS:

122653955

PSOM:

113302893

OSA:	4350521 4352145

DOSA:

Os12t0443000-00(Os12g0443000)

OBR:	102715166 121055701

OGL:	127753973 127757822

BDI:

100823617

ATS:

109782849

TDC:	119288066 119349182

TAES:

123064325 123142111 123182780

TUA:

125525901

LPER:

127313585

LRD:	124656937 124683420

SBI:

110435249

ZMA:	100381980 103652921(ts5)

SITA:

101768903 101774825

SVS:	117850389 117866210

PVIR:

120644341 120667857 120686613 120699353

PHAI:

112901932

PDA:

103706541

EGU:

105051167

MUS:	103979622 103989822 103996040

ZOF:	121980387 121983194 121984288 121985453 122040032 122048591

DCT:	110093521 110093522

PEQ:

110018426

MSIN:

131235395 131241424 131246876

NCOL:

116246669 116247098 116249972 116250319 116251037

ATR:

18438139

» show all

Reference

1 [PMID:21576464]

Authors

Koo AJ, Cooke TF, Howe GA

Title

Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine.

Journal

Proc Natl Acad Sci U S A 108:9298-303 (2011)
DOI:10.1073/pnas.1103542108

Sequence

[ath:AT3G48520]

Reference

2 [PMID:21849397]

Authors

Kitaoka N, Matsubara T, Sato M, Takahashi K, Wakuta S, Kawaide H, Matsui H, Nabeta K, Matsuura H

Title

Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.

Journal

Plant Cell Physiol 52:1757-65 (2011)
DOI:10.1093/pcp/pcr110

Reference

3 [PMID:22215670]

Authors

Heitz T, Widemann E, Lugan R, Miesch L, Ullmann P, Desaubry L, Holder E, Grausem B, Kandel S, Miesch M, Werck-Reichhart D, Pinot F

Title

Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.

Journal

J Biol Chem 287:6296-306 (2012)
DOI:10.1074/jbc.M111.316364

Sequence

[ath:AT3G48520]

Reference

4 [PMID:24467969]

Authors

Kitaoka N, Kawaide H, Amano N, Matsubara T, Nabeta K, Takahashi K, Matsuura H

Title

CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.

Journal

Phytochemistry 99:6-13 (2014)
DOI:10.1016/j.phytochem.2013.12.019

Reference

5 [PMID:25210037]

Authors

Koo AJ, Thireault C, Zemelis S, Poudel AN, Zhang T, Kitaoka N, Brandizzi F, Matsuura H, Howe GA

Title

Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis.

Journal

J Biol Chem 289:29728-38 (2014)
DOI:10.1074/jbc.M114.603084

Sequence

[ath:AT5G63450 AT3G48520]

Reference

6 [PMID:26164240]

Authors

Widemann E, Grausem B, Renault H, Pineau E, Heinrich C, Lugan R, Ullmann P, Miesch L, Aubert Y, Miesch M, Heitz T, Pinot F

Title

Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.

Journal

Phytochemistry 117:388-99 (2015)
DOI:10.1016/j.phytochem.2015.06.027

Sequence

[ath:AT3G48520]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.48

IUBMB Enzyme Nomenclature:

1.14.14.48

ExPASy - ENZYME nomenclature database:

1.14.14.48

BRENDA, the Enzyme Database:

1.14.14.48

DBGET integrated database retrieval system