Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

12-hydroxyjasmonoyl-L-amino acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)

a 12-hydroxyjasmonoyl-L-amino acid + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = a 12-hydroxy-12-oxojasmonoyl-L-amino acid + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:R11807];
(1a) a 12-hydroxyjasmonoyl-L-amino acid + [reduced NADPH---hemoprotein reductase] + O2 = a 12-oxojasmonoyl-L-amino acid + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11808];
(1b) a 12-oxojasmonoyl-L-amino acid + [reduced NADPH---hemoprotein reductase] + O2 = a 12-hydroxy-12-oxojasmonoyl-L-amino acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11809]

R11807 R11808 R11809
Reaction

12-hydroxyjasmonoyl-L-amino acid [CPD:C21696];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
12-oxojasmonoyl-L-amino acid [CPD:C21699]

A cytochrome P450 (heme thiolate) enzyme found in plants. The enzyme acts on jasmonoyl-L-amino acid conjugates that have been hydroxylated at the C-12 position of jasmonic acid by EC 1.14.14.48, jasmonoyl-L-amino acid 12-hydroxylase, further oxidizing that position to a carboxylate via an aldehyde intermediate. While the best studied substrate is (+)-7-epi-jasmonoyl-L-isoleucine, the enzyme was shown to be active with jasmonoyl-L-phenylalanine, and is likely to be active with other jasmonoyl-amino acid conjugates.

EC 1.14.14.49 created 2017

1  [PMID:22215670]

2  [PMID:26164240]

3  [PMID:27459369]