Entry
Name
syn-pimaradiene 3-monooxygenase;
CYP701A8
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
9beta-pimara7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (9beta-pimara-7,15-diene-3beta-ol-forming)
Reaction(IUBMB)
9beta-pimara-7,15-diene + [reduced NADPH---hemoprotein reductase] + O2 = 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R11980 ]
Reaction(KEGG)
Substrate
9beta-pimara-7,15-diene [CPD:
C18225 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ]
Product
9beta-pimara-7,15-diene-3beta-ol [CPD:
C21825 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from rice, Oryza sativa.
History
EC 1.14.14.68 created 2018
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K21719 ent-sandaracopimaradiene 3-hydroxylase / syn-pimaradiene 3-monooxygenase
Genes
DOSA : Os06t0568600-01(Os06g0568600) Os06t0569500-01(Os06g0569500)
Taxonomy
Reference
Authors
Kitaoka N, Wu Y, Xu M, Peters RJ
Title
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
Journal
Other DBs