Entry
Name
ent-cassadiene hydroxylase;
CYP71Z7
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
ent-cassa-12,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-3beta-hydroxycassa-12,15-dien-2-one-forming)
Reaction(IUBMB)
ent-cassa-12,15-diene + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:
R11981 ];
(1a) ent-cassa-12,15-diene + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R11982 ];
(1b) ent-cassa-12,15-dien-2beta-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:
R11983 ];
(1b') ent-cassa-12,15-dien-2beta-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R11985 ];
(1c) ent-cassa-12,15-dien-2-one + [reduced NADPH---hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R11984 ];
(1c') ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH---hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:
R11986 ]
Reaction(KEGG)
Substrate
ent-cassa-12,15-diene [CPD:
C18226 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ];
ent-cassa-12,15-dien-2beta-ol [CPD:
C21827 ];
(1b') ent-cassa-12,15-dien-2beta-ol;
ent-cassa-12,15-dien-2-one [CPD:
C21828 ];
(1c') ent-cassa-12,15-diene-2beta,3beta-diol
Product
ent-3beta-hydroxycassa-12,15-dien-2-one [CPD:
C21826 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ];
ent-cassa-12,15-dien-2beta-ol [CPD:
C21827 ];
ent-cassa-12,15-dien-2-one [CPD:
C21828 ];
ent-cassa-12,15-diene-2beta,3beta-diol [CPD:
C21829 ]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the plant Oryza sativa (rice) that is involved in phytocassanes biosynthesis. Depending on the order of activities, the enzyme may form either ent-cassa-12,15-dien-2-one or ent-cassa-12,15-diene-2beta,3beta-diol as an intermediate.
History
EC 1.14.14.69 created 2018
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K21291 ent-cassadiene hydroxylase
Genes
DOSA : Os02t0570700-01(Os02g0570700)
Taxonomy
Reference
Authors
Kitaoka N, Wu Y, Xu M, Peters RJ
Title
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
Journal
Other DBs