KEGG   ENZYME: 1.14.14.71
Entry
EC 1.14.14.71               Enzyme                                 
Name
cucurbitadienol 11-hydroxylase;
CYP87D18
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
cucurbitadienol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (11-oxocucurbitadienol-forming)
Reaction(IUBMB)
cucurbitadienol + 2 [reduced NADPH---hemoprotein reductase] + 2 O2 = 11-oxocucurbitadienol + 2 [oxidized NADPH---hemoprotein reductase] + 3 H2O (overall reaction) [RN:R12007];
(1a) cucurbitadienol + [reduced NADPH---hemoprotein reductase] + O2 = 11-hydroxycucurbitadienol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12008];
(1b) 11-hydroxycucurbitadienol + [reduced NADPH---hemoprotein reductase] + O2 = 11-oxocucurbitadienol + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R12009]
Reaction(KEGG)
R12007 R12008 R12009
Substrate
cucurbitadienol [CPD:C19819];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
11-hydroxycucurbitadienol [CPD:C21842]
Product
11-oxocucurbitadienol [CPD:C21841];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
11-hydroxycucurbitadienol [CPD:C21842]
Comment
Isolated from the plant Siraitia grosvenorii (monk fruit).
History
EC 1.14.14.71 created 2018
Pathway
ec00996  Biosynthesis of various alkaloids
ec01110  Biosynthesis of secondary metabolites
Orthology
K25060  cucurbitadienol 11-hydroxylase / 11-oxocucurbitadienol 20-hydroxylase
Genes
CSV: 101213710
CMO: 103482802
BHJ: 120083895
CMAX: 111490271
CMOS: 111457186
CPEP: 111802347
Reference
1  [PMID:26903528]
  Authors
Zhang J, Dai L, Yang J, Liu C, Men Y, Zeng Y, Cai Y, Zhu Y, Sun Y
  Title
Oxidation of Cucurbitadienol Catalyzed by CYP87D18 in the Biosynthesis of Mogrosides from Siraitia grosvenorii.
  Journal
Plant Cell Physiol 57:1000-7 (2016)
DOI:10.1093/pcp/pcw038
  Sequence
[ag:AEM42986]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.71
IUBMB Enzyme Nomenclature: 1.14.14.71
ExPASy - ENZYME nomenclature database: 1.14.14.71
BRENDA, the Enzyme Database: 1.14.14.71

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Pathway (4)   
   KEGG PATHWAY (4)   
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (6)   
   KEGG REACTION (3)   
   KEGG RCLASS (3)   
Gene (8)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (7)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (31)   

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