Entry Name ent-isokaurene C2/C3-hydroxylase;
Class Oxidoreductases;BRITE hierarchy
Sysname ent-isokaurene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-isokaurene-2beta,3beta-diol-forming)
Reaction(IUBMB) ent-isokaurene + 2 O2 + 2 [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:
R12058 ];
(1a) ent-isokaurene + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokauren-2beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R09861 ];
(1b) ent-isokauren-2beta-ol + O2 + [reduced NADPH---hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R11978 ]
Reaction(KEGG) Substrate ent-isokaurene [CPD:
C20145 ];
O2 [CPD:
C00007 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
ent-isokauren-2beta-ol [CPD:
C20146 ]
Product ent-isokaurene-2beta,3beta-diol [CPD:
C21831 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ];
ent-isokauren-2beta-ol [CPD:
C20146 ]
Comment This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
History EC 1.14.14.76 created 2012 as EC 1.14.13.143, transferred 2018 to EC 1.14.14.76
Pathway ec01110 Biosynthesis of secondary metabolites
Orthology K16083 ent-isokaurene hydroxylase
Genes DOSA : Os02t0570500-01(Os02g0570500)
Taxonomy Reference Authors Wu Y, Hillwig ML, Wang Q, Peters RJ
Title Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.
Journal Sequence Reference Authors Kitaoka N, Wu Y, Xu M, Peters RJ
Title Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
Journal Other DBs
