KEGG ENZYME: 1.14.14.82

ENZYME: 1.14.14.82

Entry

EC 1.14.14.82               Enzyme

Name

flavonoid 3'-monooxygenase;
CYP75B1 (gene name);
flavonoid 3'-hydroxylase;
flavonoid 3-hydroxylase (incorrect);
NADPH:flavonoid-3'-hydroxylase (incorrect);
flavonoid 3-monooxygenase (incorrect)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

flavonoid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)

Reaction(IUBMB)

a flavonoid + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03615]

Reaction(KEGG)

R03615 > R02442 R03124 R06537 R06538 R08002 R08032

Substrate

flavonoid [CPD:C01579];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

3'-hydroxyflavonoid [CPD:C02790];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA.

History

EC 1.14.14.82 created 1983 as EC 1.14.13.21, transferred 2018 to EC 1.14.14.82

Pathway

ec00941

Flavonoid biosynthesis

ec00944

Flavone and flavonol biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K05280

flavonoid 3'-monooxygenase

Genes

ATH:	AT5G07990(TT7)

ALY:

9309404

CRB:

17881114

CSAT:

104708304 104734816 104769003

EUS:	EUTSA_v10013289mg

BRP:

103847118

BNA:	106419056 125574786

BOE:

106313667

RSZ:

108814778

THJ:

104801243

CPAP:

110811144

CIT:

102620909

CIC:	CICLE_v10019637mg

PVY:	116116966 116134244

MINC:

123204634

TCC:

18604820

GRA:

105764705

GHI:	107946535 107951234

GAB:

108479810

HSYR:

120155094

DZI:	111289514 111290609 111303535

EGR:	104420423 104420432 104420450 120290178

GMX:	100777999 100778531 100779588 100807293 547475(SF3'H1)

GSJ:	114392946 114411811 114411812 114411813 114416612

PVU:	PHAVU_003G1855001 PHAVU_009G192400g

VRA:	106756729 106766418

VAR:	108329986 108336235

VUN:

114164840

VUM:	124828505 124830858 124830859

CCAJ:

109787887 109787893 109797283 109807203 109808745

APRC:

113846100 113848078 113848080 113848081 113852790

MTR:	11425400 11443726 25488708 25493879

TPRA:

123884828 123917490 123917493

CAM:	101507543 101510693

PSAT:

127073732 127085494

VVO:	131604603 131627758 131627768 131634888 131638826 131656961 131656964 131659463 131662631 131662633 131662634 131662638

LJA:	Lj1g3v2293820.1(Lj1g3v2293820.1) Lj4g3v1708650.1(Lj4g3v1708650.1) Lj4g3v2267360.1(Lj4g3v2267360.1)

ADU:

107471019

AIP:

107623580

AHF:	112716295 112783881

LANG:

109344553 109353774

PCIN:

129308142 129308176 129309718 129309724 129320287

QSA:	O6P43_002210

RCN:

112178545

PPER:

18777306

PMUM:

103338591

PAVI:

110753332 110762285

PDUL:

117627102

MDM:	103437875 103455413

MSYL:

126599791 126625207

PXB:

103930751

ZJU:

107419096

MNT:	21388201 21388202

CSAV:

115709313 115709845 115709933

CSV:

101207160

CMO:

103494036

BHJ:

120070880

CMAX:

111499893

CMOS:

111440987

CPEP:

111792334

RCU:

8284440

JCU:

105640611

HBR:	110635515 110636257

MESC:

110617654

POP:

7482418

PEU:

105115867

PALZ:

118047241

JRE:

108982908

CILL:

122281663

CAVE:

132174935 132176604

QSU:

112039462

QLO:

115964033

TWL:	119981407 119987383

VVI:	100232999(F3'H) 100263810

VRI:

117904722

SLY:

101266618

SPEN:

107015315

SOT:

102593656

SSTN:

125857755

SDUL:

129895184

CANN:

107862334

LBB:

132632867

NTA:	107773493(NtF3'H) 107795677(F3'H1)

NSY:	104221951 104236297

NTO:

104093246

NAU:

109223789

INI:	109160581(Magenta)

ITR:	116028975 116028976

SIND:

105157984

OEU:

111401587

EGT:	105958663 105973421

SSPL:

121762367

SMIL:

131008844 131020359

SHIS:

125195331

APAN:

127240258 127240266 127255223 127255224 127255225

HAN:	110873618 110908215 110908322

ECAD:

122579393 122580625 122606651

LSV:	111891078 111908262

CCAV:

112514430

DCR:

108216209

CSIN:

114310487

RVL:	131299187 131299194

AEW:

130750375

BVG:

104907876

SOE:

110789886

CQI:	110700687 110726355

ATRI:

130798260

MOF:

131145532

NNU:	104597960 104598992

MING:

122075299

TSS:

122654067

PSOM:

113275817 113314103 113323649

OSA:	4348299 4348304

DOSA:

Os10t0317900-01(Os10g0317900) Os10t0320100-01(Os10g0320100)

OBR:	102704205 102716721

OGL:	127786089 127786240

BDI:	100832738 100840140 100844617 104583406

ATS:	109737789 109767455 109773560

TDC:	119295285 119319554 119322175 119329613 119338089 119349858 119366542

TAES:

123042898 123070997 123101915 123133137 123148568 123162411 123167460 123183410

TUA:	125518733 125526476 125532439

LPER:

127307462 127312600

LRD:	124667643 124684077 124684146 124687121

SBI:	8056468 8070594 8070599 8072541

ZMA:	100273383 100279887 103653707

SITA:

101759053 101763802

SVS:	117835112 117836868

PVIR:

120650957 120651359

PHAI:

112878303 112903334

PDA:	103708250 103710250

EGU:	105055971 105058071

MUS:

103979831

ZOF:	121970404 121975478 122042133 122046667

DCT:	110095936 110096779 110096781 110113263 110113268 110115941

PEQ:	110019582 110022396 110031953 110031993 110032025 110038151

AOF:	109819797 109835873 109837072

MSIN:

131228644

NCOL:

116256966 116257503 116257504 116257698 116257842

ATR:

18428045

» show all

Reference

Authors

Forkmann, G., Heller, W. and Grisebach, H.

Title

Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.

Journal

Z Naturforsch C: Biosci 35:691-695 (1980)

Reference

2 [PMID:10504566]

Authors

Brugliera F, Barri-Rewell G, Holton TA, Mason JG.

Title

Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.

Journal

Plant J 19:441-51 (1999)
DOI:10.1046/j.1365-313X.1999.00539.x

Sequence

[ag:AAD56282]

Reference

3 [PMID:11030432]

Authors

Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B

Title

Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.

Journal

Biol Chem 381:749-53 (2000)
DOI:10.1515/BC.2000.095

Sequence

[ath:AT5G07990]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.82

IUBMB Enzyme Nomenclature:

1.14.14.82

ExPASy - ENZYME nomenclature database:

1.14.14.82

BRENDA, the Enzyme Database:

1.14.14.82

CAS:	75991-44-5

DBGET integrated database retrieval system