Entry
Name
4'-methoxyisoflavone 2'-hydroxylase;
CYP81E1 (gene name);
CYP81E3 (gene name);
CYP81E7 (gene name);
isoflavone 2'-monooxygenase (ambiguous);
isoflavone 2'-hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
formononetin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
formononetin + [reduced NADPH---hemoprotein reductase] + O2 = 2'-hydroxyformononetin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R06560 ]
Reaction(KEGG)
Substrate
formononetin [CPD:
C00858 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ]
Product
2'-hydroxyformononetin [CPD:
C02920 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ]
Comment
A cytochrome P-450 (heme-thiolate) protein. Acts on isoflavones with a 4'-methoxy group, such as formononetin and biochanin A. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain. EC
1.14.14.90 , isoflavone 2'-hydroxylase, is less specific and acts on other isoflavones as well as 4'-methoxyisoflavones.
History
EC 1.14.14.89 created 1992 as EC 1.14.13.53, modified 2005, transferred 2018 to EC 1.14.14.89
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K13260 isoflavone/4'-methoxyisoflavone 2'-hydroxylase
Genes
PVY : 116109383 116120583 116120646 116121581 116125665
MINC : 123198536 123198601 123198617 123198618 123198753 123198756 123203003
TCC : 18601853 18601857 18601860 18601861
GHI : 107916731 107936806 107945765 107953970 107953971
HSYR : 120119725 120171065 120213447
DZI : 111282012 111282131 111282224 111282978 111283036 111283932 111308417
GMX : 100784650 100790507 100797371 100798269 100801092(CYP81E11) 100804509 100807819 100811727 100812102(I2'H)
GSJ : 114367022 114367290 114368609 114373520 114390631 114404224 114424679 114425468 114425469 114425838
PVU : PHAVU_002G014700g PHAVU_002G014800g PHAVU_002G187700g PHAVU_004G068400g PHAVU_009G244000g PHAVU_009G244100g PHAVU_009G244200g PHAVU_010G012900g PHAVU_010G013000g PHAVU_010G013100g
VRA : 106758033 106759715 106760052 106760053 106761763 106764429 106766213 106773653 106773682 106773765 106773766 106774281 106774974 106776630 106777449 106778042
VAR : 108319103 108321061 108321087 108321100 108321119 108321122 108334554 108335141 108343787 108344905 108345040 108346675 108346914 108347035 108347128
VUN : 114163099 114163100 114163781 114164214 114165712 114165763 114167228 114167561 114173717 114174265 114174626 114175001 114181477 114182143 114182199 114182463 114182619
VUM : 124819551 124819859 124819860 124820099 124821473 124824847 124824851 124824852 124831492 124834207 124835762 124835763 124841436 124842976 124842977
CCAJ : 109790994 109792385 109793792 109794026 109794035 109806902 109811112
APRC : 113849543 113857907 113858397 113867105 113869706
MTR : 112421340 11408558 11410588 25488207 25493449 25493516
TPRA : 123906371 123906372 123906373 123906374 123906377 123914423 123915916 123915927 123916196 123917341 123917604 123917667 123918669 123919792
CAM : 101489020 101502156(cyp81E3v2) 101502474(cyp81E3) 101502775 101503091 101505947 101506258(cyp81E2)
PSAT : 127074396 127105873 127107394 127120436 127120437 127120438 127127982
VVO : 131607683 131639112 131646902 131652232 131652233 131661173
LJA : Lj0g3v0281229.2(Lj0g3v0281229.2) Lj2g3v1925640.1(Lj2g3v1925640.1) Lj4g3v0189840.1(Lj4g3v0189840.1) Lj4g3v0189850.1(Lj4g3v0189850.1)
ADU : 107480513 107482081 107482401 107483699 107487681 107487702 107487703 107487739
AIP : 107627314 107632124 107638257 107639093 107642734 107642736 107642737
AHF : 112720547 112734659 112740936 112741792 112748459 112748463 112748464 112748468 112792439 112795658 112796477 112799808 112800534 112800537 112800538 112800541
LANG : 109329825 109331947 109334087 109334259 109345024 109352214 109353350 109353351 109353352 109357642
PCIN : 129284482 129293195 129293476 129293644 129293677 129321954 129321990 129322031 129323030
QSA : O6P43_024589 O6P43_033870 O6P43_033871 O6P43_033875 O6P43_033876
FVE : 101304511 101305403 101305693
RCN : 112184375 112186881 112189488 112190515
PPER : 18773681 18773715 18774146 18774496 18774581 18774650
PMUM : 103337225 103337235 103337245 103337257 103337275 103337298
PAVI : 110759579 110771616 110771618 110771619
PDUL : 117629602 117631034 117631339 117631373 117632678
MDM : 103401458 103432708 114821502
MSYL : 126602497 126606060 126616614
PXB : 103961611 103961612 103961613
ZJU : 107404429 107405743 107424872 107424876 107424878
MNT : 21392635 21392637 21392638 21392639 21392640 21392641
CSAV : 115702681 115702684 115702687 115702757 115702763 115702764 115703987 115712095 115715709
CSV : 101210967 101211829 101215449
CMO : 103484810 103484812 103495911
BHJ : 120068873 120073472 120073756 120087733 120087862 120088480 120088601 120088769 120088875 120089042 120089212 120089372
MCHA : 111005286 111017882
CMAX : 111477798 111478140 111478736 111483621
CMOS : 111448607 111452969 111453889
CPEP : 111777333 111803141 111803665
JCU : 105639782 105639783 105639785
MESC : 110629129 110629218 110629283 110629407 110629531 122723064
PALZ : 118029929 118029939 118035408 118035422 118035432 118037227 118045734 118045736 118047804 118061035
JRE : 109007026 109007027 109010646 118343650
CILL : 122309883 122309884 122311650
CAVE : 132161606 132161745 132162020 132162127 132162557 132175208 132191356 132191942 132192176
QSU : 112011650 112015862 112015863 112017242 112017245 112017252 112025873 112025878 112025881 112034277 112034317 112034321 112034346 112034347 112034350 112034362
QLO : 115951222 115951651 115951655 115951658 115952256 115952331 115952376 115952826 115952851 115952852 115953179 115953180 115953582 115953902 115954075 115983756 115985242 115986324
TWL : 119997195 119997208 119997218 120004182 120012609 120012614 120013433 120014284 120014286 120015125
VVI : 100243905 100249057 100249138 100250944 100254242 100256210 100259358 100261095 100266303 100853341
VRI : 117917263 117917285 117917291 117917293 117917766 117917770 117917771 117919082 117919083 117919383 117927910
SSTN : 125846305 125846307
CANN : 107860230 107860232 107860235
INI : 109151858 109151908 109151909
ITR : 116005602 116011712 116013580 116013655 116019781 116019782
SIND : 105162115 105162117
SSPL : 121773716 121777590 121801955 121804848 121806249
SMIL : 130988937 130988942 130990926 130990933
SHIS : 125192286 125194405 125194406 125194410 125196081 125202123
APAN : 127248449 127248896 127266512
ECAD : 122580573 122586673 122586677 122608068
CSIN : 114256462 114258440 114258443 114258460 114266005 114266006 114278468 114278477 114286449 114297578 114297592 114299362 114300533 114310199
RVL : 131298400 131302933 131303661 131303822 131303929 131304147 131304381 131304383 131304384 131304389 131331278 131331283 131331284
AEW : 130768141 130768404 130768406 130769430 130776575 130776668 130777390 130784324 130784385
ATRI : 130814919 130815482 130821740 130824092
» show all
Taxonomy
Reference
1
Authors
Hinderer, W., Flentje, U. and Barz, W.
Title
Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation.
Journal
FEBS Lett 214:101-106 (1987)
Reference
Authors
Akashi T, Aoki T, Ayabe S
Title
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.
Journal
Sequence
Reference
Authors
Liu CJ, Huhman D, Sumner LW, Dixon RA.
Title
Regiospecific hydroxylation of isoflavones by cytochrome p450 81E enzymes from Medicago truncatula.
Journal
Sequence
Other DBs