KEGG ENZYME: 1.14.14.93

ENZYME: 1.14.14.93

Entry

EC 1.14.14.93               Enzyme

Name

3,9-dihydroxypterocarpan 6a-monooxygenase;
3,9-dihydroxypterocarpan 6a-hydroxylase;
3,9-dihydroxypterocarpan 6alpha-monooxygenase (erroneous);
CYP93A1 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

(6aR,11aR)-3,9-dihydroxypterocarpan,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6a-hydroxylating)

Reaction(IUBMB)

(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03452]

Reaction(KEGG)

R03452

Substrate

(6aR,11aR)-3,9-dihydroxypterocarpan [CPD:C04271];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

(6aS,11aS)-3,6a,9-trihydroxypterocarpan [CPD:C01263];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein found in soybean. The product of the reaction is the biosynthetic precursor of the glyceollin phytoalexins.

History

EC 1.14.14.93 created 1989 as EC 1.14.13.28, transferred 2018 to EC 1.14.14.93

Pathway

ec00943

Isoflavonoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13261

3,9-dihydroxypterocarpan 6a-monooxygenase

Genes

GMX:	100776878(CYP93A1) 100808223

GSJ:	114399397 114406771

Reference

1 [PMID:6540173]

Authors

Hagmann ML, Heller W, Grisebach H.

Title

Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures.

Journal

Eur J Biochem 142:127-31 (1984)
DOI:10.1111/j.1432-1033.1984.tb08259.x

Reference

2 [PMID:9720921]

Authors

Schopfer CR, Kochs G, Lottspeich F, Ebel J

Title

Molecular characterization and functional expression of dihydroxypterocarpan 6a-hydroxylase, an enzyme specific for pterocarpanoid phytoalexin biosynthesis in soybean (Glycine max L.).

Journal

FEBS Lett 432:182-6 (1998)
DOI:10.1016/S0014-5793(98)00866-7

Sequence

[gmx:100776878]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.93

IUBMB Enzyme Nomenclature:

1.14.14.93

ExPASy - ENZYME nomenclature database:

1.14.14.93

BRENDA, the Enzyme Database:

1.14.14.93

CAS:	92584-16-2

DBGET integrated database retrieval system