KEGG   ENZYME: 1.14.14.94
Entry
EC 1.14.14.94               Enzyme                                 
Name
leukotriene-B4 20-monooxygenase;
leukotriene-B4 20-hydroxylase;
leucotriene-B4 omega-hydroxylase;
LTB4 20-hydroxylase;
LTB4 omega-hydroxylase;
CYP4F2 (gene name);
CYP4F3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH---hemoprotein reductase] + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03866]
Reaction(KEGG)
R03866
Substrate
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate [CPD:C02165];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate [CPD:C04853];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in mammals.
History
EC 1.14.14.94 created 1989 as EC 1.14.13.30, transferred 2018 to EC 1.14.14.94
Pathway
ec00590  Arachidonic acid metabolism
Orthology
K17726  phylloquinone omega-hydroxylase / docosahexaenoic acid omega-hydroxylase / leukotriene-B4 20-monooxygenase
Genes
HSA4051(CYP4F3) 8529(CYP4F2)
PTR100609185 748117(CYP4F3) 748531(CYP4F2)
PPS100967411(CYP4F2) 100969141(CYP4F3)
GGO101125692 101135418 101152881
PON100434096 100437847 100453229
NLE100579320 100588006 115836968
HMH116478624 116478627
MCC718349 719317
MCF102130440(CYP4F3) 102134301(CYP4F2)
MTHB126942252 126942253
MNI105494188 105494717
CSAB103234079 103234089
CATY105601614 105601627
PANU101005940 101010705
TGE112612404 112613097
MLEU105540101 105540126
RRO104661012 104661014
RBB108543262 108543288 108543294
TFN117076174
PTEH111554233 111554287
CANG105519773 105519778
CJC100411160 100412575
SBQ101050944 101051361
CIMI108298561(CYP4F2)
CSYR103250633
MMUR105867925 105867928 105867931
LCAT123634142 123636967 123639775
PCOQ105810118 105825072 105825741 105827104
OGA100942215 100943471 100950748 100952962
CPOC100733735
OCU100348038 100350957
TUP102483333
GVR103593427 103610570
VLG121495723
UAR123779961 123780007
LLV125083145
ZCA113917975
MLX118005050
MLF102425173 102436085
PKL118706555
DRO112305042
PDIC114503445
RFQ117038128
PGIG120606485
PVP105311420
RAY107517263
TOD119240020
LAV100657832
DNM101441145
 » show all
Reference
1  [PMID:3027095]
  Authors
Romano MC, Eckardt RD, Bender PE, Leonard TB, Straub KM, Newton JF.
  Title
Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases.
  Journal
J Biol Chem 262:1590-5 (1987)
Reference
2  [PMID:4044832]
  Authors
Shak S, Goldstein IM.
  Title
Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450.
  Journal
J Clin Invest 76:1218-28 (1985)
DOI:10.1172/JCI112077
Reference
3  [PMID:2986111]
  Authors
Soberman RJ, Harper TW, Murphy RC, Austen KF.
  Title
Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes.
  Journal
Proc Natl Acad Sci U S A 82:2292-5 (1985)
DOI:10.1073/pnas.82.8.2292
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.94
IUBMB Enzyme Nomenclature: 1.14.14.94
ExPASy - ENZYME nomenclature database: 1.14.14.94
BRENDA, the Enzyme Database: 1.14.14.94
CAS: 90119-11-2

DBGET integrated database retrieval system