KEGG ENZYME: 1.14.14.98

ENZYME: 1.14.14.98

Entry

EC 1.14.14.98               Enzyme

Name

protopine 6-monooxygenase;
protopine 6-hydroxylase;
CYP82N2 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

protopine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6-hydroxylating)

Reaction(IUBMB)

protopine + [reduced NADPH---hemoprotein reductase] + O2 = 6-hydroxyprotopine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R04699]

Reaction(KEGG)

R04699

Substrate

protopine [CPD:C05189];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

Product

6-hydroxyprotopine [CPD:C05190];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein involved in benzophenanthridine alkaloid synthesis in higher plants.

History

EC 1.14.14.98 created 1999 as EC 1.14.13.55, transferred 2018 to EC 1.14.14.98

Pathway

ec00950

Isoquinoline alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K21693

protopine 6-monooxygenase

Genes

PSOM:

113330723 113357146

Reference

Authors

Tanahashi, T. and Zenk, M.H.

Title

Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis.

Journal

Phytochemistry 29:1113-1122 (1990)

Reference

2 [PMID:22421633]

Authors

Takemura T, Ikezawa N, Iwasa K, Sato F

Title

Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells.

Journal

Phytochemistry 91:100-8 (2013)
DOI:10.1016/j.phytochem.2012.02.013

Sequence

[ag:BAK20464]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.14.98

IUBMB Enzyme Nomenclature:

1.14.14.98

ExPASy - ENZYME nomenclature database:

1.14.14.98

BRENDA, the Enzyme Database:

1.14.14.98

CAS:	128561-60-4

DBGET integrated database retrieval system