KEGG ENZYME: 1.14.15.13

ENZYME: 1.14.15.13

Entry

EC 1.14.15.13               Enzyme

Name

pulcherriminic acid synthase;
cyclo-L-leucyl-L-leucyl dipeptide oxidase;
CYP134A1;
CypX (ambiguous)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

cyclo(L-leucyl-L-leucyl),reduced-ferredoxin:oxygen oxidoreductase (N-hydroxylating,aromatizing)

Reaction(IUBMB)

cyclo(L-leucyl-L-leucyl) + 6 reduced ferredoxin + 3 O2 = pulcherriminic acid + 6 oxidized ferredoxin + 4 H2O [RN:R10322]

Reaction(KEGG)

R10322

Substrate

cyclo(L-leucyl-L-leucyl) [CPD:C20514];
reduced ferredoxin [CPD:C00138];
O2 [CPD:C00007]

Product

pulcherriminic acid [CPD:C20515];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]

Comment

A heme-thiolate (P-450) enzyme from the bacterium Bacillus subtilis. The order of events during the overall reaction is unknown. Pulcherrimic acid spontaneously forms an iron chelate with Fe(3+) to form the red pigment pulcherrimin [2].

History

EC 1.14.15.13 created 2013

Orthology

K17474

pulcherriminic acid synthase

Genes

PLU:

plu0296

PLUM:

A4R40_01445

PAKH:

B0X70_01350

PLUI:

CE143_01350

XGR:	QL128_02120

MME:	Marme_0277

BSU:	BSU35060(cypX)

BSH:	BSU6051_35060(cypX)

BSUT:

BSUB_03736(prfB)

BSUL:

BSUA_03736(prfB)

BSUS:

Q433_19190

BSQ:	B657_35060(cypX)

BSP:	U712_17560

BSS:	BSUW23_17185(cypX)

BST:

GYO_3843

BIT:	BIS30_07155

BLI:	BL00818(cypX)

BLD:	BLi03567(cypX)

BLH:	BaLi_c36290(cypX)

BMOJ:

HC660_33910(cypX)

BBY:	CY96_28935

BRY:	M0696_17750(cypX)

BACL:

BS34A_38160(cypX)

BALM:

BsLM_3513

BGI:	BGM20_11660

ACAI:

ISX45_01295

SEPS:

DP17_2386

SHA:

pSHaeC07

SSD:	SPSINT_1707

SDT:	SPSE_0788(cypX)

SDP:	NCTC12225_00488(cypX)

STRM:

M444_03610

SCZ:	ABE83_28615

SGS:	AVL59_18625

SLX:	SLAV_36095(cypX2)

SLK:	SLUN_30550

SVN:	CP980_02950

SCHA:

CP983_42200

SNF:	JYK04_00990(cypX)

SYAN:

NRK68_28880

SGK:	PET44_02185

KAB:	B7C62_06170

» show all

Reference

1 [PMID:5837792]

Authors

MacDonald JC

Title

Biosynthesis of pulcherriminic acid.

Journal

Biochem J 96:533-8 (1965)
DOI:10.1042/bj0960533

Reference

2 [PMID:20690619]

Authors

Cryle MJ, Bell SG, Schlichting I

Title

Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.

Journal

Biochemistry 49:7282-96 (2010)
DOI:10.1021/bi100910y

Sequence

[bsu:BSU35060]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.13

IUBMB Enzyme Nomenclature:

1.14.15.13

ExPASy - ENZYME nomenclature database:

1.14.15.13

BRENDA, the Enzyme Database:

1.14.15.13

DBGET integrated database retrieval system