KEGG ENZYME: 1.14.15.14

ENZYME: 1.14.15.14

Entry

EC 1.14.15.14               Enzyme

Name

methyl-branched lipid omega-hydroxylase;
CYP124

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

methyl-branched lipid,reduced-ferredoxin:oxygen oxidoreductase (omega-hydroxylating)

Reaction(IUBMB)

a methyl-branched lipid + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = an omega-hydroxy-methyl-branched lipid + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster

Substrate

methyl-branched lipid;
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]

Product

omega-hydroxy-methyl-branched lipid;
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Comment

The enzyme, found in pathogenic and nonpathogenic mycobacteria species, actinomycetes, and some proteobacteria, hydroxylates the omega-carbon of a number of methyl-branched lipids, including (2E,6E)-farnesol, phytanate, geranylgeraniol, 15-methylpalmitate and (2E,6E)-farnesyl diphosphate. It is a P-450 heme-thiolate enzyme.

History

EC 1.14.15.14 created 2015

Orthology

K20497

methyl-branched lipid omega-hydroxylase

Genes

MTU:	Rv2266(cyp124)

MTV:

RVBD_2266

MTC:

MT2328

MRA:	MRA_2286(cyp124)

MTF:	TBFG_12293

MTB:	TBMG_01716

MTK:	TBSG_01726

MTZ:	TBXG_001698

MTG:	MRGA327_13975

MTE:	CCDC5079_2102

MTUR:

CFBS_2399(cyp124)

MTL:	CCDC5180_2074

MTO:	MTCTRI2_2301(cyp124)

MTD:	UDA_2266(cyp124)

MTN:	ERDMAN_2490(cyp124)

MTJ:	J112_12165

MTUB:

MT7199_2297(cyp124)

MTUC:

J113_15800

MTUE:

J114_12155

MTX:	M943_11730

MTUH:

I917_15990

MTUL:

TBHG_02216

MTUT:

HKBT1_2390(cyp124)

MTUU:

HKBT2_2392(cyp124)

MTQ:	HKBS1_2396(cyp124)

MBO:	BQ2027_MB2289(cyp124)

MBB:	BCG_2283(cyp124)

MBT:	JTY_2277(cyp124)

MBM:	BCGMEX_2271(cyp124)

MBK:	K60_023510

MBX:

BCGT_2089

MAF:	MAF_22760(cyp124)

MMIC:

RN08_2514

MCE:	MCAN_22901(cyp124)

MCQ:	BN44_50212(cyp)

MCV:	BN43_31508(cyp)

MCX:	BN42_40188(cyp)

MCZ:	BN45_50597(cyp)

MORY:

MO_002375(cyp124)

MPA:

MAP_2015

MAO:

MAP4_1810

MAVI:

RC58_09040

MAVU:

RE97_09045

MAV:

MAV_2174

MIT:

OCO_21580

MIA:

OCU_21760

MID:

MIP_03049

MYO:

OEM_19490

MCHI:

AN480_11180

MIR:

OCQ_20680

MMAL:

CKJ54_09630

MMAN:

MMAN_18210(cyp124_1)

MSA:	Mycsm_03362

MUL:	MUL_1282(cyp124A1)

MMI:	MMAR_3361(cyp124A1)

MMAE:

MMARE11_32610(cyp124A1)

MLI:	MULP_03625(cyp124A1)

MSHO:

MSHO_47750(cyp124)

MMC:	Mmcs_2742 Mmcs_2991

MKM:	Mkms_2786 Mkms_3035

MJL:	Mjls_2772 Mjls_3006

MMM:

W7S_10140

MKN:	MKAN_03480

MYV:	G155_16190

MYE:	AB431_15885 AB431_16625

MHAD:

B586_09820

MDX:	BTO20_16125

MSHG:

MSG_03191(cyp124)

MFJ:	MFLOJ_00260(cyp124) MFLOJ_59110

MSTO:

MSTO_00890(cyp124)

MSIM:

MSIM_44970(cyp124)

MSAK:

MSAS_12420(cyp124)

MKU:	I2456_16160

MLW:	MJO58_16940

MXE:	MYXE_20490(cyp124_2)

MNV:	MNVI_45420(cyp124)

MPAG:

C0J29_18745

MGOR:

H0P51_17465

MCOO:

MCOO_13040(cyp124)

MBAI:

MB901379_03033

MSEO:

MSEO_19780(cyp124)

MHEK:

JMUB5695_02065(cyp124_3)

MGAU:

MGALJ_16330(cyp124_1) MGALJ_19280(cyp124_2)

MLJ:	MLAC_22790(cyp124)

MBRD:

MBRA_58180(cyp124)

MSHJ:

MSHI_09980(cyp124)

MMAM:

K3U93_14985

MSPG:

F6B93_14060

MOT:	LTS72_07685

MKY:	IWGMT90018_23910(cyp124)

MADI:

A7U43_15515

MWU:	PT015_07575

MSM:	MSMEG_3551

MSG:	MSMEI_3470(cyp124)

MSB:	LJ00_17675

MSN:	LI99_17680

MSH:	LI98_17685

MVA:

Mvan_3029

MGI:

Mflv_3307

MSP:	Mspyr1_26350

MCB:	Mycch_2589 Mycch_2853

MNE:	D174_15450

MYN:	MyAD_15175

MFT:	XA26_33180

MPHL:

MPHLCCUG_02433

MVQ:

MYVA_3042

MLL:	B1R94_13480

MRH:	MycrhN_4629 MycrhN_4917

MTHN:

4412656_02310(cyp124_2)

MDU:	MDUV_12270

MCHT:

MCHIJ_09550(cyp124_1) MCHIJ_38170(cyp124_2)

MAUU:

NCTC10437_02924

MMAG:

MMAD_29660

MMOR:

MMOR_33770(cyp124)

MAIC:

MAIC_28850(cyp124)

MALV:

MALV_06760

MTY:	MTOK_12370(cyp124) MTOK_47990

MPSC:

MPSYJ_35430

MARZ:

MARA_09040 MARA_52060

MGAD:

MGAD_05490(cyp124_1) MGAD_08500(cyp124_2)

MHEV:

MHEL_52500(cyp124)

MSAR:

MSAR_39650

MANY:

MANY_14570

MAUB:

MAUB_06780 MAUB_40940

MPOF:

MPOR_34320

MPHU:

MPHO_05900

MMUC:

C1S78_003945 C1S78_015380

MMAT:

MMAGJ_65750

MBOK:

MBOE_41250 MBOE_47370(cyp124_1)

MFG:	K6L26_15710

MSEI:

MSEDJ_42730

MFLV:

NCTC10271_02487

MCEE:

MCEL_07530(cyp124)

MMON:

EWR22_14565 EWR22_15755

MGO:	AFA91_22075

MHOL:

K3U96_12440

MSEN:

K3U95_13655

MPAE:

K0O64_14945 K0O64_15740

MRF:	MJO55_14425

MDF:	K0O62_15555

MCRO:

MI149_15940 MI149_16695

MGRO:

FZ046_20300

NYU:	D7D52_16470

SVL:	Strvi_3964

SRC:	M271_05620

SMAL:

SMALA_7272

SSOI:

I1A49_40125

SBH:	SBI_08399(nanP)

SGM:	GCM10017557_10840

SAUO:

BV401_39970

SBY:	H7H31_00670

SMOB:

J7W19_02520

SANT:

QR300_04700

STRZ:

OYE22_00750

NGV:	CDO52_10130

STRR:

EKD16_17690

TCU:

Tcur_1580

ACTW:

F7P10_06655 F7P10_07660 F7P10_18535 F7P10_22425 F7P10_41715

AGRA:

AGRA3207_001650 AGRA3207_007609 AGRA3207_007747

AMAZ:

LUW76_11095 LUW76_12175 LUW76_24135 LUW76_47310

ACIR:

OG979_00250 OG979_02055 OG979_29305 OG979_30400

SPIQ:

OHA34_11825 OHA34_12685

ACTQ:

OG417_21475 OG417_41270

SRO:	Sros_7140 Sros_7141 Sros_7142

NOA:	BKM31_16295 BKM31_57925 BKM31_57940

NOW:	GBF35_38505

NCX:	Nocox_31440 Nocox_31455

NGN:	LCN96_42915

NFS:	OIE67_06300

TBI:

Tbis_2431

MHAI:

OHB01_39405

FRI:	FraEuI1c_5327

BSD:	BLASA_4033(cyp)

MMAR:

MODMU_4611(cyp)

APRT:

MUY14_02400

PDX:

Psed_5473

PSEE:

FRP1_01500

PSEQ:

AD006_09125 AD006_31290

PECQ:

AD017_16955 AD017_29945

PHH:	AFB00_14855

PBRO:

HOP40_11870 HOP40_25115

PPEL:

H6H00_11890

ACTY:

OG774_01385

CAI:

Caci_0813

ABAI:

IMCC26256_111881

AYM:	YM304_17460 YM304_26560 YM304_26850

ATQ:	GH723_04580

IAM:	HC251_05490

IMA:	PO878_06865

DHI:	LH044_15500 LH044_17515

» show all

Reference

1 [PMID:19933331]

Authors

Johnston JB, Kells PM, Podust LM, Ortiz de Montellano PR

Title

Biochemical and structural characterization of CYP124: a methyl-branched lipid omega-hydroxylase from Mycobacterium tuberculosis.

Journal

Proc Natl Acad Sci U S A 106:20687-92 (2009)
DOI:10.1073/pnas.0907398106

Sequence

[mtu:Rv2266]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.14

IUBMB Enzyme Nomenclature:

1.14.15.14

ExPASy - ENZYME nomenclature database:

1.14.15.14

BRENDA, the Enzyme Database:

1.14.15.14

All links

Gene (1269)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (233)   
   KEGG MGENES (875)   
   RefGene (160)   
Protein sequence (66)   
   UniProt (63)   
   SWISS-PROT (3)   
DNA sequence (30)   
   GenBank (15)   
   EMBL (15)   
Protein domain (3)   
   InterPro (3)   
All databases (1368)   

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