KEGG   ENZYME: 1.14.15.19
Entry
EC 1.14.15.19               Enzyme                                 
Name
C-19 steroid 1alpha-hydroxylase;
CYP260A1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
testosterone,reduced-ferredoxin:oxygen oxidoreductase (1alpha-hydroxylating)
Reaction(IUBMB)
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11349]
Reaction(KEGG)
R11349
Substrate
testosterone [CPD:C00535];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
1alpha-hydroxytestosterone [CPD:C21296];
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
The enzyme, characterized from the bacterium Sorangium cellulosum, is a class I cytochrome P-450, and uses ferredoxin as its electron donor [1]. It was shown to act on several C-19 steroid substrates, including testosterone, androstenedione, testosterone-acetate and 11-oxoandrostenedione [2].
History
EC 1.14.15.19 created 2016
Reference
1  [PMID:19696019]
  Authors
Ewen KM, Hannemann F, Khatri Y, Perlova O, Kappl R, Krug D, Huttermann J, Muller R, Bernhardt R
  Title
Genome mining in Sorangium cellulosum So ce56: identification and characterization of the homologous electron transfer proteins of a myxobacterial  cytochrome P450.
  Journal
J Biol Chem 284:28590-8 (2009)
DOI:10.1074/jbc.M109.021717
Reference
2  [PMID:26478560]
  Authors
Khatri Y, Ringle M, Lisurek M, von Kries JP, Zapp J, Bernhardt R
  Title
Substrate Hunting for the Myxobacterial CYP260A1 Revealed New 1alpha-Hydroxylated Products from C-19 Steroids.
  Journal
Chembiochem 17:90-101 (2016)
DOI:10.1002/cbic.201500420
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.19
IUBMB Enzyme Nomenclature: 1.14.15.19
ExPASy - ENZYME nomenclature database: 1.14.15.19
BRENDA, the Enzyme Database: 1.14.15.19

  All links  
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (4)   
   InterPro (4)   
All databases (15)   

Download RDF
DBGET integrated database retrieval system