KEGG ENZYME: 1.14.15.22

ENZYME: 1.14.15.22

Entry

EC 1.14.15.22               Enzyme

Name

vitamin D 1,25-hydroxylase;
CYP105A1;
Streptomyces griseolus cytochrome P450SU-1

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

calciol,ferredoxin:oxygen oxidoreductase (1,25-hydroxylating)

Reaction(IUBMB)

(1) calciol + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = calcidiol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11458];
(2) calcidiol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = calcitriol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11459]

Reaction(KEGG)

R11458 R11459

Substrate

calciol [CPD:C05443];
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
calcidiol [CPD:C01561]

Product

calcidiol [CPD:C01561];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
calcitriol [CPD:C01673]

Comment

A P-450 (heme-thiolate) enzyme found in the bacterium Streptomyces griseolus. cf. EC 1.14.14.24, vitamin D 25-hydroxylase and EC 1.14.15.18, calcidiol 1-monooxygenase.

History

EC 1.14.15.22 created 2016

Pathway

ec00100

Steroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K21146

vitamin D 1,25-hydroxylase