KEGG   ENZYME: 1.14.15.23
Entry
EC 1.14.15.23               Enzyme                                 
Name
chloroacetanilide N-alkylformylase;
cndA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
butachlor,ferredoxin:oxygen oxidoreductase (butyl formate-releasing)
Reaction(IUBMB)
butachlor + 2 reduced ferredoxin [iron-sulfur] cluster + O2 = 2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11812]
Reaction(KEGG)
R11812
Substrate
butachlor [CPD:C10931];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007]
Product
2-chloro-N-(2,6-diethylphenyl)acetamide [CPD:C21712];
butyl formate [CPD:C21713];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Sphingomonas sp. DC-6, initiates the degradation of several chloroacetanilide herbicides, including alachlor, acetochlor, and butachlor. The enzyme is a Rieske non-heme iron oxygenase, and requires a ferredoxin and EC 1.18.1.3, ferredoxin---NAD+ reductase, for activity.
History
EC 1.14.15.23 created 2017
Orthology
K22325  chloroacetanilide N-alkylformylase
Genes
SPHDHY78_30640
Reference
1  [PMID:24928877]
  Authors
Chen Q, Wang CH, Deng SK, Wu YD, Li Y, Yao L, Jiang JD, Yan X, He J, Li SP
  Title
Novel three-component Rieske non-heme iron oxygenase system catalyzing the N-dealkylation of chloroacetanilide herbicides in sphingomonads DC-6 and DC-2.
  Journal
Appl Environ Microbiol 80:5078-85 (2014)
DOI:10.1128/AEM.00659-14
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.23
IUBMB Enzyme Nomenclature: 1.14.15.23
ExPASy - ENZYME nomenclature database: 1.14.15.23
BRENDA, the Enzyme Database: 1.14.15.23

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