KEGG ENZYME: 1.14.15.32

ENZYME: 1.14.15.32

Entry

EC 1.14.15.32               Enzyme

Name

pentalenene oxygenase;
PtlI

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

pentalenene,reduced ferredoxin:oxygen 13-oxidoreductase

Reaction(IUBMB)

pentalenene + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = pentalen-13-al + 4 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction) [RN:R09814];
(1a) pentalenene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-ol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R09812];
(1b) pentalen-13-ol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-al + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R09813]

Reaction(KEGG)

R09812 R09813 R09814

Substrate

pentalenene [CPD:C01841];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007];
pentalen-13-ol [CPD:C19938]

Product

pentalen-13-al [CPD:C19939];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
pentalen-13-ol [CPD:C19938]

Comment

A cytochrome P-450 (heme-thiolate) protein found in the bacterium Streptomyces avermitilis. The enzyme is involved in the biosynthesis of pentalenolactone and related antibiotics.

History

EC 1.14.15.32 created 2011 as EC 1.14.13.133, transferred 2018 to EC 1.14.15.32

Pathway

ec00909

Sesquiterpenoid and triterpenoid biosynthesis

ec00998

Biosynthesis of various antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15907

pentalenene oxygenase

Genes

SMA:	SAVERM_2999(ptlI)

SBH:	SBI_09680(cyp48)

SDV:	BN159_1442(ptlI)

SCI:	B446_29700

SCW:	TU94_27410

SLE:	sle_13150(sle_13150)

SCLF:

BB341_00225

SSIA:

A7J05_09235

SALF:

SMD44_00690(ptlI)

SALJ:

SMD11_0076(ptlI) SMD11_6153(ptlI)

SFEU:

IM697_14465

SGJ:	IAG43_27160

SHK:	J2N69_35800

SFP:	QUY26_08865 QUY26_37035

SCT:	SCAT_p0090(ptlI)

SCY:	SCATT_p16540

KSK:

KSE_03600

» show all

Reference

1 [PMID:17017767]

Authors

Quaderer R, Omura S, Ikeda H, Cane DE

Title

Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis.

Journal

J Am Chem Soc 128:13036-7 (2006)
DOI:10.1021/ja0639214

Sequence

[sma:SAVERM_2999]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.32

IUBMB Enzyme Nomenclature:

1.14.15.32

ExPASy - ENZYME nomenclature database:

1.14.15.32

BRENDA, the Enzyme Database:

1.14.15.32

DBGET integrated database retrieval system