KEGG ENZYME: 1.14.15.34

ENZYME: 1.14.15.34

Entry

EC 1.14.15.34               Enzyme

Name

20-oxo-5-O-mycaminosyltylactone 23-monooxygenase;
tylH1 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

20-oxo-5-O-beta-mycaminosyltylactone,reduced ferredoxin:oxygen oxidoreductase (23-hydroxylating)

Reaction(IUBMB)

20-oxo-5-O-beta-mycaminosyltylactone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 5-O-beta-mycaminosyltylonolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R10656]

Reaction(KEGG)

R10656

Substrate

20-oxo-5-O-beta-mycaminosyltylactone [CPD:C20760];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

5-O-beta-mycaminosyltylonolide;
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein. Involved in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria.

History

EC 1.14.15.34 created 2014 as EC 1.14.13.186, transferred 2018 to EC 1.14.15.34

Pathway

ec00522

Biosynthesis of 12-, 14- and 16-membered macrolides

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15991

20-oxo-5-O-mycaminosyltylactone 23-monooxygenase