KEGG   ENZYME: 1.14.19.41
Entry
EC 1.14.19.41               Enzyme                                 
Name
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Reaction(IUBMB)
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]
Reaction(KEGG)
R07506;
(other) R07489 R11097
Substrate
ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15780];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
History
EC 1.14.19.41 created 2015
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09831  sterol 22-desaturase
K09832  sterol 22-desaturase
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: 9317081 9317405 9317406
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106432759 106447495 125585615 125608302 125609361
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
CIC: CICLE_v10014973mg
PVY: 116141721
MINC: 123192456 123218316
TCC: 18596516
GRA: 105769297 105769298 105772027 105774040
GHI: 107901888 107905877 107905878 107942637 121203644
GAB: 108452825 108452842
HSYR: 120131393 120190641 120194923
DZI: 111280976 111318668
EGR: 104440796
GMX: 100787616 100808148
GSJ: 114377625 114387884
PVU: PHAVU_006G163800g
VRA: 106776160
VAR: 108340983
VUN: 114188144
VUM: 124834997
CCAJ: 109789198
APRC: 113852140
MTR: 11437817
TPRA: 123898150 123905820
CAM: 101489453
PSAT: 127079474
VVO: 131595535
ADU: 107468177
AIP: 107623676
AHF: 112714308 112782610
LANG: 109328906 109329075 109345270
PCIN: 129286005
QSA: O6P43_012958
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
PAVI: 110749808 110773983
PDUL: 117625864
MDM: 103434989
MSYL: 126621236
ZJU: 107413371
MNT: 21404536
CSV: 101221194
CMO: 103484375
BHJ: 120074625
CMAX: 111478743 111496674
CMOS: 111433344 111433531
CPEP: 111782177 111803380
HBR: 110640583 110645097
MESC: 110609904 110621253
POP: 7472572
PEU: 105117943
PALZ: 118050840
JRE: 109021374
CILL: 122275110
CAVE: 132171142
QSU: 112009839
QLO: 115980600 115992293
TWL: 119995149
VVI: 100255505
VRI: 117922864
SLY: 100136886(CYP710A11)
SPEN: 107011895
SOT: 102581211
SSTN: 125848721
SDUL: 129874504
CANN: 107859112
LBB: 132623336
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
ITR: 116017561
SIND: 105167297 105172499
OEU: 111407278 111412227
EGT: 105951371
SSPL: 121805298 121808148
SMIL: 131013605
SHIS: 125194367
APAN: 127254813
HAN: 110911670 110937398
ECAD: 122593276
LSV: 111890946
CCAV: 112504656
DCR: 108199974
CSIN: 114313482
RVL: 131312423 131312424 131312426
AEW: 130755298
BVG: 104883528
SOE: 110778797 110778903
CQI: 110730201 110732522
ATRI: 130814489 130823996
MOF: 131144302
NNU: 104597733
MING: 122060078 122092751
TSS: 122639679 122650136
PSOM: 113329902 113338741 113345304 113355287
OSA: 4325562 4325563 4325564 9267882
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
OBR: 102709230 102711741
OGL: 127759857 127760109 127760169 127760847
BDI: 100827562
ATS: 109746138 109757150 109772439
TDC: 119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120
TAES: 123060655 123069249 123077753 123094069 123099277 123157569 123162932
TUA: 125507657 125543216
LPER: 127294839 127296147 127296148 127321331
LRD: 124647277 124658988 124663956 124663963 124663967 124664979 124667667 124668114 124695192 124695193
SBI: 8079522
ZMA: 107305676
SITA: 101764868 101770311
SVS: 117837101 117858509
PVIR: 120676345 120705614
PHAI: 112893543
PDA: 103702545 103703021
EGU: 105040643 105048162
MUS: 103995072 103999677 104000847
ZOF: 121989158 121998106 122052204 122056687
DCT: 110110071
PEQ: 110029296
AOF: 109828814
MSIN: 131245570
NCOL: 116265070
ATR: 18435199
SMO: SELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)
PPP: 112277322 112285903
SCE: YMR015C(ERG5)
SEUB: DI49_4102
SPAO: SPAR_M01390
AGO: AGOS_ABR053C
KLA: KLLA0_D11242g
KMX: KLMA_60417(cyp524A1)
LTH: KLTH0D07832g
VPO: Kpol_1056p10
ZRO: ZYRO0E06204g
CGR: CAGL0M07656g
NCS: NCAS_0A12700(NCAS0A12700)
NDI: NDAI_0B01560(NDAI0B01560)
TPF: TPHA_0I02420(TPHA0I02420)
TBL: TBLA_0H02650(TBLA0H02650)
TGB: HG536_0G01250
TDL: TDEL_0B06510(TDEL0B06510)
KAF: KAFR_0A02110(KAFR0A02110)
KNG: KNAG_0M02100(KNAG0M02100)
ZMK: HG535_0G03560
PPA: PAS_chr3_0507
DHA: DEHA2D06182g
PIC: PICST_73043(ERG5)
PGU: PGUG_05085
SPAA: SPAPADRAFT_63544(ERG5)
LEL: PVL30_005321(ERG5)
CAL: CAALFM_C702840CA(ERG5)
CTP: CTRG_04983
COT: CORT_0G04140
CDU: CD36_72510
CTEN: CANTEDRAFT_115386
YLI: YALI0A18062g
CLU: CLUG_04246
CLUS: A9F13_04g02805
CAUR: CJI96_0004850 CJI96_0004910
SLB: AWJ20_75(ERG5)
PKZ: C5L36_0B12520
BNN: FOA43_004793(ERG5)
BBRX: BRETT_002777(ERG5)
OPA: HPODL_01958
SLUD: SCDLUD_004216
NCR: NCU05278
NTE: NEUTE1DRAFT116692(NEUTE1DRAFT_116692)
SMP: SMAC_01710
PAN: PODANSg6262 PODANSg7995
PBEL: QC761_202070 QC761_304420(ERG5)
PPSD: QC762_202070 QC762_304420(ERG5)
PPSP: QC763_202070 QC763_304420(ERG5)
PPSA: QC764_202070 QC764_304420(ERG5)
TTT: THITE_128412
MTM: MYCTH_73679
CTHR: CTHT_0029760
MGR: MGG_06047
PPEI: PpBr36_02869
PGRI: PgNI_01835
TMN: UCRPA7_7946
SSCK: SPSK_00476
FGR: FGSG_01959 FGSG_03686
FPU: FPSE_01847 FPSE_09181
FPOA: FPOAC1_002075 FPOAC1_006089
FVN: FVRRES_02319 FVRRES_06686
FVR: FVEG_07284 FVEG_08786
FOX: FOXG_04166 FOXG_09685
NHE: NECHADRAFT_72313 NECHADRAFT_72652
FFC: NCS54_01269600 NCS54_01305200
FKR: NCS57_01239900 NCS57_01291900
FMU: J7337_010705 J7337_012541
TRE: TRIREDRAFT_122166 TRIREDRAFT_58689
TRR: M419DRAFT_141218 M419DRAFT_74177
MAW: MAC_08636
MAJ: MAA_01109
PCHM: VFPPC_03546
CMT: CCM_02962
AMUS: LMH87_000586 LMH87_006973
PLJ: VFPFJ_00660 VFPFJ_08344
PTKZ: JDV02_004750 JDV02_007982(ERG5)
VAL: VDBG_00192 VDBG_00755
VDA: VDAG_00367 VDAG_08476
CFJ: CFIO01_05732 CFIO01_13734
CLUP: CLUP02_04776 CLUP02_14903
CHIG: CH63R_04552 CH63R_14243
SAPO: SAPIO_CDS4161 SAPIO_CDS7005
ELA: UCREL1_11694
PFY: PFICI_04403 PFICI_13034
SSL: SS1G_09832
BFU: BCIN_07g01120(Bcerg5)
MBE: MBM_06688
PSCO: LY89DRAFT_257563 LY89DRAFT_584410
GLZ: GLAREA_02023 GLAREA_06590
ANI: ANIA_04042
AFM: AFUA_1G03950
ACT: ACLA_008020 ACLA_030640
NFI: NFIA_020740
AOR: AO090003000253 AO090003000954
ANG: An01g02810 An11g03230
AFV: AFLA_000720 AFLA_001436
ALUC: AKAW2_20450S(ERG5_1) AKAW2_80298S(ERG5_2)
ACHE: ACHE_11570S(ERG5)
APUU: APUU_40308A(ERG5) APUU_60358A
PCS: N7525_005484 N7525_010907
PDP: PDIP_21270
POU: POX_e06874
TMF: EYB26_008188
TRG: TRUGW13939_00550 TRUGW13939_08662
CIM: CIMG_05009
CPW: CPC735_068810
URE: UREG_06546
PBL: PAAG_11073(PAAG_00230)
PBN: PADG_07898
ABE: ARB_03814
TVE: TRV_05093
AJE: HCAG_06013
BGH: BDBG_03201
PNO: SNOG_06055
PTE: PTT_19871
BZE: COCCADRAFT_88203
BSC: COCSADRAFT_221542
BOR: COCMIDRAFT_93916
AALT: CC77DRAFT_991604
ARAB: EKO05_0000970
ZTR: MYCGRDRAFT_55438(CYP-70)
PFJ: MYCFIDRAFT_49450
FFU: CLAFUR5_02386
CBET: CB0940_03149
BCOM: BAUCODRAFT_489380
NPA: UCRNP2_2755
TML: GSTUM_00005882001
SPO: SPAC19A8.04(erg5)
SOM: SOMG_03716(erg5)
CNE: CNF03720
CNB: CNBF1100
CNG: CNAG_06644
CGI: CGB_F1500C
CDEU: CNBG_1921
TMS: TREMEDRAFT_40696
TASA: A1Q1_00690
CCAC: CcaHIS019_0405230(ERG5)
TVS: TRAVEDRAFT_140384
DSQ: DICSQDRAFT_66936
PCO: PHACADRAFT_246788
SHS: STEHIDRAFT_123767
HIR: HETIRDRAFT_141009(cpm111)
PSQ: PUNSTDRAFT_59236
ADL: AURDEDRAFT_109660
FME: FOMMEDRAFT_106824 FOMMEDRAFT_155371
GTR: GLOTRDRAFT_53715
RSX: RhiXN_01858
LBC: LACBIDRAFT_187166(ERG5)
CCI: CC1G_01671
ABP: AGABI1DRAFT66440(AGABI1DRAFT_66440)
ABV: AGABI2DRAFT189837(AGABI2DRAFT_189837)
PCUB: JR316_0000711
MRR: Moror_12729
MORE: E1B28_009893
SCM: SCHCO_02609502(SCHCODRAFT_02609502)
CPUT: CONPUDRAFT_94730
SLA: SERLADRAFT_459032
WSE: WALSEDRAFT_69870
WIC: J056_001310
UMA: UMAG_00350
PFP: PFL1_03644
SGRA: EX895_000577
MGL: MGL_0310
MRT: MRET_0994
MSYM: MSY001_2710
 » show all
Reference
1  [PMID:8543054]
  Authors
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE
  Title
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61,  a sterol delta 22-desaturase.
  Journal
FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3
Reference
2  [PMID:8635732]
  Authors
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M
  Title
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
  Journal
Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9
  Sequence
[sce:YMR015C]
Reference
3  [PMID:16531502]
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
[ath:AT2G34500 AT2G34490]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.41
IUBMB Enzyme Nomenclature: 1.14.19.41
ExPASy - ENZYME nomenclature database: 1.14.19.41
BRENDA, the Enzyme Database: 1.14.19.41

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Pathway (7)   
   KEGG PATHWAY (6)   
   KEGG MODULE (1)   
Chemical substance (11)   
   KEGG COMPOUND (11)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (679)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (463)   
   KEGG MGENES (68)   
   RefGene (146)   
Protein sequence (1543)   
   UniProt (1519)   
   SWISS-PROT (11)   
   RefSeq(pep) (13)   
DNA sequence (72)   
   RefSeq(nuc) (25)   
   GenBank (23)   
   EMBL (24)   
Protein domain (5)   
   InterPro (5)   
All databases (2322)   

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