KEGG   ENZYME: 1.14.19.46
Entry
EC 1.14.19.46               Enzyme                                 
Name
sn-1 linoleoyl-lipid 6-desaturase;
desD (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
1-linoleoyl-2-acyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (6,7-cis-dehydrogenating)
Reaction(IUBMB)
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
1-linoleoyl-2-acyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
1-gamma-linolenoyl-2-acyl-[glycerolipid];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme, characterized from cyanobacteria, introduces a cis double bond at carbon 6 of linoleoyl groups (18:2) attached to the sn-1 position of glycerolipids. The enzyme is a front-end desaturase, introducing the new double bond between a pre-existing double bond and the carboxyl-end of the fatty acid. It is nonspecific with respect to the polar head group of the glycerolipid.
History
EC 1.14.19.46 created 2015
Reference
1  [PMID:12231903]
  Authors
Higashi S, Murata N
  Title
An in Vivo Study of Substrate Specificities of Acyl-Lipid Desaturases and Acyltransferases in Lipid Synthesis in Synechocystis PCC6803.
  Journal
Plant Physiol 102:1275-1278 (1993)
DOI:10.1104/pp.102.4.1275
Reference
2  [PMID:9630958]
  Authors
Reddy AS, Thomas TL
  Title
Expression of a cyanobacterial delta 6-desaturase gene results in gamma-linolenic acid production in transgenic plants.
  Journal
Nat Biotechnol 14:639-42 (1996)
DOI:10.1038/nbt0596-639
Reference
3  [PMID:16328883]
  Authors
Kurdrid P, Subudhi S, Hongsthong A, Ruengjitchatchawalya M, Tanticharoen M
  Title
Functional expression of Spirulina-Delta6 desaturase gene in yeast, Saccharomyces cerevisiae.
  Journal
Mol Biol Rep 32:215-26 (2005)
DOI:10.1007/s11033-005-0416-y
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.46
IUBMB Enzyme Nomenclature: 1.14.19.46
ExPASy - ENZYME nomenclature database: 1.14.19.46
BRENDA, the Enzyme Database: 1.14.19.46

  All links  
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (2)   
   InterPro (2)   
All databases (6)   

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