KEGG ENZYME: 1.14.19.79

ENZYME: 1.14.19.79

Entry

EC 1.14.19.79               Enzyme

Name

3beta,22alpha-dihydroxysteroid 3-dehydrogenase;
CYP90A1 (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

3beta,22alpha-dihydroxysteroid,[reduced NADPH-hemoprotein reductase]:oxygen 3-oxidoreductase

Reaction(IUBMB)

(1) (22S)-22-hydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2 = (22S)-22-hydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O [RN:R07446];
(2) 6-deoxoteasterone + [reduced NADPH-hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + 2 H2O [RN:R12965]

Reaction(KEGG)

R07446 R12965

Substrate

(22S)-22-hydroxycampesterol [CPD:C15795];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007];
6-deoxoteasterone [CPD:C15799]

Product

(22S)-22-hydroxycampest-4-en-3-one [CPD:C15796];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]

Comment

This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Arabidopsis thaliana, catalyses C-3 dehydrogenation of all 3beta-hydroxy brassinosteroid synthesis intermediates with 22-hydroxylated or 22,23-dihydroxylated side chains.

History

EC 1.14.19.79 created 2022

Pathway

ec00905

Brassinosteroid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K09588

3beta,22alpha-dihydroxysteroid 3-dehydrogenase

Genes

ATH:	AT5G05690(CPD)

ALY:

9309287

CRB:

17884348

CSAT:

104708544 104734575 104768762

EUS:	EUTSA_v10013418mg

BRP:	103846910 103850594 103855637

BNA:	106380838 106386485 106419352 106422628 106440690 106442975

BOE:	106318190 106322573

RSZ:	108816976 108841893 108845671

THJ:	104816550 104823148

CPAP:

110819283

CIT:

102608450

CIC:	CICLE_v10031576mg

PVY:

116111729

MINC:

123192846 123227420

TCC:

18585886

GRA:	105773896 105776399

GHI:	107931824 121203585 121222638 121230741

GAB:	108464090 108484595

HSYR:

120115401 120119816 120196138 120206255 120213509

DZI:

111310123

EGR:	104421095 104454392

GMX:	100775886 100781291 100789842 100795125(CYP90A15)

GSJ:	114382781 114382937 114395558 114399102

PVU:	PHAVU_001G242200g PHAVU_008G224600g

VRA:	106757945 106763863

VAR:	108329739 108336275

VUN:	114162864 114193700

VUM:	124840902 124840912 124848030

CCAJ:

109790810 109805365

APRC:

113847685 113850044

MTR:	11405199 11411219

TPRA:

123894763 123924766

CAM:	101495460 101502086

PSAT:

127083957 127118165

VVO:	131621017 131661944

LJA:	Lj2g3v2746640.1(Lj2g3v2746640.1) Lj6g3v0524000.1(Lj6g3v0524000.1)

ADU:	107457722 107469471 107469499

AIP:

107623684

AHF:	112702383 112715406 112715407 112765910 112784909

LANG:

109338900 109345069

PCIN:

129294677 129299421 129301969

QSA:	O6P43_013869

FVE:

101301332

RCN:

112182961

PPER:

18785088

PMUM:

103332773

PAVI:

110749731

PDUL:

117617356

MDM:	103432823 103437230

MSYL:

126593345 126630456

PXB:	103936683 103958600

ZJU:

107413844

MNT:

21409069

CSAV:

115705581

CSV:

101216594

CMO:

103501437

BHJ:

120081203

MCHA:

111023728

CMAX:

111484767 111490939

CMOS:

111458347

CPEP:

111782190 111796648

RCU:

8261035

JCU:

105646342

HBR:	110646453 110651697

MESC:

110619393 110624089 122724866

POP:	7474440 7494485

PEU:	105120258 105133596 105139650

PALZ:

118043321 118055866

JRE:	108983029 108991904

CILL:

122291997 122301489

CAVE:

132188504

QSU:

112011086

TWL:	120014341 120015942

VVI:

100258692

VRI:

117929163

SLY:	101255105(CYP90A5)

SPEN:

107023593

SOT:

102604556

SSTN:

125874177

SDUL:

129899171

CANN:

107873297

LBB:

132608956

NTA:	107785062(CYP90) 107791859

NSY:

104236880

NTO:

104097076

NAU:

109234452

INI:	109148237 109186937

ITR:	116000029 116013913

SIND:

105160880

OEU:

111380564

EGT:

105977877

SSPL:

121773410 121776177 121796729

SMIL:

131005712

SHIS:

125206039 125221189

APAN:

127256502 127259788

HAN:	110872702 110912986

ECAD:

122585038 122586969

LSV:	111896789 111919719

CCAV:

112509778 112525947

DCR:

108210725

CSIN:

114303283

RVL:

131320543

AEW:	130762959 130764251 130774808 130774809 130792753

BVG:

104903459

SOE:	110785503 110802180

CQI:	110699265 110714661 110725236 110733431

ATRI:

130803046

MOF:

131165932

NNU:	104595015 104601254

MING:

122093477

TSS:

122672797

PSOM:

113313306 113314280 113323522 113338994

OSA:	4349746 4351461

DOSA:

Os11t0143200-01(Os11g0143200) Os12t0139300-01(Os12g0139300)

OBR:	102701391 102719724

OGL:	127755491 127758233

BDI:

100831598

ATS:

109745725

TDC:	119302433 119308324

TAES:

123103027 123111228 123120303

TUA:

125508677

LPER:

127302648

LRD:	124652755 124652988

SBI:

8085636

ZMA:	100272666 103647105

SITA:

101755217 101768081

SVS:	117865869 117866170

PVIR:

120643525 120663533 120696674

PHAI:

112876861 112884910 112901899

PDA:

103716536

EGU:

105046233

MUS:	103977709 103994415

ZOF:	122027304 122028724 122036630 122037555

DCT:	110101203 110108808

PEQ:	110022516 110034891 110035737

AOF:

109829756

MSIN:

131244004

NCOL:

116253817 116256159

ATR:

18437942

» show all

Reference

1 [PMID:22822057]

Authors

Ohnishi T, Godza B, Watanabe B, Fujioka S, Hategan L, Ide K, Shibata K, Yokota T, Szekeres M, Mizutani M.

Title

CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation.

Journal

J Biol Chem 287:31551-60 (2012)
DOI:10.1074/jbc.M112.392720

Other DBs

ExplorEnz - The Enzyme Database:

1.14.19.79

IUBMB Enzyme Nomenclature:

1.14.19.79

ExPASy - ENZYME nomenclature database:

1.14.19.79

BRENDA, the Enzyme Database:

1.14.19.79

DBGET integrated database retrieval system