Entry
Name
6beta-hydroxyhyoscyamine epoxidase;
hydroxyhyoscyamine dioxygenase;
(6S)-6-hydroxyhyoscyamine,2-oxoglutarate oxidoreductase (epoxide-forming)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
BRITE hierarchy
Sysname
(6S)-6beta-hydroxyhyoscyamine,2-oxoglutarate:oxygen oxidoreductase (epoxide-forming)
Reaction(IUBMB)
(6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 = scopolamine + succinate + CO2 + H2O [RN:
R03737 ]
Reaction(KEGG)
Substrate
(6S)-6beta-hydroxyhyoscyamine;
2-oxoglutarate [CPD:
C00026 ];
O2 [CPD:
C00007 ]
Product
Comment
Requires Fe2+ and ascorbate.
History
EC 1.14.20.13 created 1992 as EC 1.14.11.14, transferred 2018 to EC 1.14.20.13
Pathway
ec00960 Tropane, piperidine and pyridine alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K12692 hyoscyamine (6S)-dioxygenase / 6beta-hydroxyhyoscyamine epoxidase
Reference
1
Authors
Hashimoto, T., Kohno, J. and Yamada, Y.
Title
6beta-Hydroxyhyoscyamine epoxidase from cultured roots of Hyoscyamus niger.
Journal
Phytochemistry 28:1077-1082 (1989)
Other DBs