KEGG ENZYME: 1.14.99.44

ENZYME: 1.14.99.44

Entry

EC 1.14.99.44               Enzyme

Name

diapolycopene oxygenase;
crtP (ambiguous)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

4,4'-diapolycopene,AH2:oxygen oxidoreductase (4,4'-hydroxylating)

Reaction(IUBMB)

4,4'-diapolycopene + 4 reduced acceptor + 4 O2 = 4,4'-diapolycopenedial + 4 acceptor + 6 H2O [RN:R09671]

Reaction(KEGG)

R09671;
(other) R07654 R09727 R09728

Substrate

4,4'-diapolycopene [CPD:C19797];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

4,4'-diapolycopenedial [CPD:C19798];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

Little activity with neurosporene or lycopene. Involved in the biosynthesis of C30 carotenoids such as staphyloxanthin. The enzyme oxidizes each methyl group to the hydroxymethyl and then a dihydroxymethyl group, followed by the spontaneous loss of water to give an aldehyde group.

History

EC 1.14.99.44 created 2011

Pathway

ec00906

Carotenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K10210

diapolycopene oxygenase

Genes

MMT:	Metme_3774

MDN:	JT25_002365

MDH:	AYM39_15930

MKO:	MKLM6_3290

METL:

U737_16970(crtI)

MPAD:

KEF85_14155(crtI)

MELL:

IVG45_20685(crtI)

MRP:	NM686_003115(crtI)

MPSY:

CEK71_14805

KUS:	B9G99_06335

KMA:	B9H00_08720

BROS:

QUH67_09675(crtI)

MEA:	Mex_1p3665(ctrI)

MDI:	METDI4240(ctrI)

MEX:

Mext_3436

MCH:

Mchl_3745

MPO:

Mpop_3632

MZA:	B2G69_24740

MRD:	Mrad2831_5031

MET:

M446_3156

MOR:

MOC_5734

META:

Y590_16875

MAQU:

Maq22A_c06955

MPHY:

MCBMB27_04475

MEE:	DA075_07760

METD:

C0214_18825

METX:

A3862_05965

METI:

DK427_17340

MMES:

MMSR116_03635(crtI)

MTEA:

DK419_25140

MIND:

mvi_47440

MOG:	MMB17_18215(crtI)

MTAD:

M6G65_29725(crtI)

MOC:	BB934_38295

MTEZ:

HPT29_002680(crtI)

MLD:	U0023_10055(crtI)

BBAR:

RHAL1_01931

LIZ:	LGH83_14110(crtI)

PLCG:

RVY76_15970(crtI)

LVR:	T8T21_16645(crtI)

GOH:

B932_2266

GAL:	A0U94_05050

GTI:	FXF46_11910(crtI) FXF46_15790(crtI)

ABG:	Asbog_01465(crtI)

KBA:	A0U89_05405

RGI:	RGI145_04005

ROS:	CTJ15_13405

RMUC:

FOB66_05790(crtI)

RFL:

Rmf_41720

NCH:	A0U93_12590

NTN:	D5366_08600(crtI)

LCK:	HN018_16745(crtI)

SRF:	LHU95_04720(crtI)

ALI:	AZOLI_p10672

ABS:	AZOBR_150005

ABQ:	ABAZ39_01055

ABF:	AMK58_25210

AZT:	TSH58p_12600

AZM:	DM194_17000

AOZ:	HUE56_02795(crtI)

AARE:

D3093_08140(crtI)

AZS:	E6C72_21090(crtI)

SKT:	IGS68_00375(crtI)

SMUC:

JL100_001155(crtI)

SROE:

JL101_000380(crtI)

PIJ:	QEJ31_08545

SAQI:

AXG55_09130

BPU:

BPUM_2690

BPUM:

BW16_14510

BPUS:

UP12_13760

BIF:	N288_02870

BWH:	A9C19_15125 A9C19_17020

BXI:	BK049_13585

BALT:

CFN77_14445(crtI)

BAER:

BAE_01975

BSAF:

BSL056_15160

BACW:

QR42_13585

BZH:	NF868_12620(crtI)

BHAI:

KJK41_04925(crtI)

BCO:	Bcell_3994

BHK:	B4U37_06820 B4U37_10405

NTX:	NQZ71_19180

BMQ:	BMQ_2021(crtI) BMQ_2949

BMD:	BMD_1977(crtI) BMD_2978

BMH:	BMWSH_2233 BMWSH_3258(crtI)

BMEG:

BG04_4322(crtP) BG04_5481(crtI)

PKS:	IE339_15755(crtI)

BLE:	BleG1_3942

SHUA:

PQ477_07380(crtI)

BPF:	BpOF4_14565(crtP)

BKO:	CKF48_16145

CGOT:

J1899_01870(crtI)

CSUA:

IM538_13645(crtI)

CSPG:

LS684_17935(crtI) LS684_23205(crtI)

OIH:

OB2459

AFL:

Aflv_2195

AGN:	AFK25_03165

LGY:	T479_06405

HHD:	HBHAL_1313 HBHAL_2763(crtNb)

HMN:	HM131_12850 HM131_19705

HLI:	HLI_02855 HLI_09930

TAP:	GZ22_08185

TAID:

KS242_09280(crtI)

VHL:	BME96_14530

VIK:	KFZ58_17670

FPN:	ABE65_002095 ABE65_002110

SJE:	AAV35_007820 AAV35_012005

BMUR:

ABE28_002620 ABE28_013810

PPSR:

I6J18_04190(crtI)

PSYH:

D0S48_20355(crtI)

GSM:	MUN87_19315(crtI)

POF:	GS400_07995(crtI)

PCHU:

QNI29_08900(crtI)

MEKU:

HUW50_18265(crtI)

MLIT:

KDJ21_022810(crtI)

RMF:	D5E69_12340(crtI)

RAZ:	U9J35_04195(crtI) U9J35_12805(crtI)

BBEV:

BBEV_0577(crtP-1) BBEV_1363(crtP-2)

BSE:	Bsel_1103 Bsel_2212

MCUI:

G8O30_05935(crtI)

PSUA:

FLK61_37095(crtI)

ECTO:

MUG87_02965(crtI) MUG87_16915(crtI)

AHAL:

FTX54_013980(crtI)

SA2351

SAV:

SAV2564

SAW:

SAHV_2548

SAH:	SaurJH1_2640

SAJ:	SaurJH9_2586

SAM:

MW2485

SAS:

SAS2450

SAR:

SAR2646

SAC:

SACOL2579

SAX:	USA300HOU_2559

SAA:	SAUSA300_2501

SAO:	SAOUHSC_02881

SAE:	NWMN_2464(crtI)

SAD:

SAAV_2630

SUU:	M013TW_2538

SUV:	SAVC_11700

SUE:	SAOV_2609c

SUJ:	SAA6159_02460

SUK:	SAA6008_02603(crtI)

SUC:	ECTR2_2417(crtI)

SUT:	SAT0131_02775

SUQ:	HMPREF0772_10625(crtI)

SUZ:	MS7_2571(crtP)

SUD:	ST398NM01_2615

SUX:	SAEMRSA15_24670

SUW:	SATW20_26860

SUG:

SAPIG2615

SUF:	SARLGA251_23390

SAUA:

SAAG_00385

SAUE:

RSAU_002405

SAUN:

SAKOR_02556

SAUS:

SA40_2318

SAUU:

SA957_2402

SAUG:

SA268_2480

SAUZ:

SAZ172_2664

SAUT:

SAI1T1_2019090

SAUJ:

SAI2T2_1019100

SAUK:

SAI3T3_1019090

SAUQ:

SAI4T8_1019100

SAUV:

SAI7S6_1019090(crtP)

SAUW:

SAI5S5_1019030(crtP)

SAUX:

SAI6T6_1019040(crtP)

SAUY:

SAI8T7_1019070(crtP)

SAUF:

X998_2547

SAB:

SAB2437c

SUY:	SA2981_2501

SAUB:

C248_2622

SAUM:

BN843_26010

SAUC:

CA347_2640(crtP)

SAUR:

SABB_01120(crtP)

SAUI:

AZ30_13435

SAUD:

CH52_06180

SAMS:

NI36_12830

SHA:

SH0488

SHH:	ShL2_00389(crtI)

SLG:	SLGD_00185

SLN:	SLUG_01830

SXY:	BE24_11840 BE24_13830

SXL:	SXYLSMQ121_0051

SXO:	SXYL_00051(crtP)

SCAP:

AYP1020_1735(crtP)

SEQO:

SE1039_25810

SPET:

CEP67_08105

SNL:	BJD96_00585

SKL:	C7J89_02350

SHOM:

EGX58_06050

SCAR:

DWB96_01935

SARL:

SAP2_00910(crtP)

SPIC:

SAMEA4384060_0087(crtP)

SSH:	NCTC13712_02520(crtP)

SSIM:

SAMEA4384339_2370(crtP)

SDB:	CNQ82_01430

SLLO:

ISP08_00310

SPSD:

JMB28_00820

STAS:

HYI43_02280

SURY:

MUA21_00255

SDEV:

Q2T90_05260(crtI)

SROT:

ML435_12330

SSCU:

CEP64_13160

MLEN:

H3V22_08605(crtI)

MACR:

BHM04_10275

MBRU:

MGG13_00650(crtI)

MEPI:

KFV12_00570(crtI)

MEQU:

KFV11_00695(crtI)

MBOC:

QSV55_01045

LGZ:	NCTC10812_02741(crtP)

ESI:	Exig_0517 Exig_0738

EAT:	EAT1b_2163 EAT1b_2493

EAN:

Eab7_0711

EXM:	U719_02710 U719_03910

EXU:	ESP131_05155 ESP131_06330

EAP:	KB235_12905(crtI) KB235_14645(crtI)

EACE:

KKI46_02915(crtI) KKI46_04105(crtI)

EAUR:

NMQ00_13555(crtI)

EMM:	PTI97_01780(crtI) PTI97_03580(crtI)

EPF:	OE059_11120(crtI) OE059_12955(crtI)

PMS:	KNP414_02931

PMQ:	PM3016_3171

PMW:

B2K_16040

PBD:	PBOR_33875

PAEN:

P40081_35205

PAEQ:

R50912_31835

PAEA:

R70723_30700

PAEE:

R70331_29455

PBV:	AR543_14100

PXL:	BS614_00330

PSWU:

SY83_14360 SY83_16585

PLUT:

EI981_26715(crtI)

PBK:	Back11_13210(crtP)

PBAC:

HUB98_14510(crtI)

PTRI:

KDC22_30830(crtI)

PALO:

E6C60_1954

PALR:

HGI30_11850(crtI) HGI30_22120(crtI)

PKA:	PQ456_11180(crtI) PQ456_20115(crtI)

PUI:	PUW25_14430(crtI)

PMAH:

PTQ21_02555(crtI)

PPOG:

QPK24_14585(crtI) QPK24_21975(crtI)

PAUN:

MJA45_20680(crtI) MJA45_20735(crtI)

PHK:	SK066_03090(crtI)

PNK:	AASFL403_07785(crtI) AASFL403_21560

PPAB:

KET34_31505(crtI)

COHN:

KCTCHS21_30250(crtP_1) KCTCHS21_30350(crtP_2)

SACA:

FFV09_16680(crtI)

SIV:

SSIL_3759

SSIL:

SOLI23_19230

PLN:	Plano_0604 Plano_2714

PKU:	AUO94_02190 AUO94_13335

PRT:	AUC31_06455 AUC31_12730

PLL:	I858_004200 I858_009375

PANA:

BBH88_05400 BBH88_17560

PDG:	BCM40_01765 BCM40_12610

PHC:	BBI08_10745 BBI08_16110

PMAR:

B0X71_03860

PPLA:

BBI15_09585 BBI15_15430

PFAE:

AJGP001_03885 AJGP001_15430

PLX:	CW734_04965 CW734_15685

PMAT:

BBI11_04595 BBI11_15115

PDEC:

H1Q58_03735(crtI) H1Q58_14400(crtI)

JEO:	JMA_07510 JMA_18970

SURE:

SporoP32a_09250

SPOS:

DV702_13350(crtI)

PANC:

E2636_00235(crtI)

PLAY:

DNR44_006400(crtI) DNR44_016995(crtI)

LFB:	C1X05_05355

TVU:	AB849_000755

KPUL:

GXN76_05120(crtI)

ECAS:

ECBG_01335

EMU:

EMQU_0489

EGA:	AL523_06010

EIE:	ENLAB_20230

CRN:	CAR_c19220(crtP)

CAW:	Q783_08810

MARR:

BKP56_12405

JEP:	BW721_07800

JDA:	BW727_102018(crtP)

JEM:	LZ578_02810(crtI) LZ578_05080(crtI)

DINC:

QNK01_06855

RRS:	RoseRS_1811

RCA:

Rcas_2535

CAU:

Caur_2410

CHL:	Chy400_2597

CAG:

Cagg_2983

HAU:

Haur_0609

CAP:	CLDAP_20440

PMET:

G4Y79_17140(crtI)

CCZ:	CCALI_00231

CCOT:

CCAX7_45230

ROO:	G5S37_15655(crtI)

SOA:	G3M56_005565(crtI)

VEG:	SAMN05444156_1435

RBA:

RB11943

PIR:	VN12_20065(crtP)

RUL:	UC8_21150(crtP)

RML:	FF011L_10680(crtP)

ROL:	CA51_34620(crtP)

RUV:	EC9_36060(crtP)

AHEL:

Q31a_22280(crtP)

RLC:	K227x_30590(crtP)

SMAM:

Mal15_20880(crtP)

SNEP:

Enr13x_54150(crtP)

PND:	Pla175_40250(crtP)

PLM:

Plim_2740

PEH:	Spb1_35070(crtP)

PLS:	VT03_32435(crtP)

PLH:	VT85_17065(crtP)

ACAF:

CA12_25840(crtP)

SVP:	Pan189_37000(crtP)

CHYA:

V22_09350(crtP_1)

PVAR:

SH412_005255(crtI)

CCOP:

Mal65_22990(crtP)

GES:	VT84_20055(carA2)

GOG:	C1280_20095

GMS:	SOIL9_30650

TIM:	GMBLW1_01360

LRS:	PX52LOC_03471

FTJ:

FTUN_7583

ULI:	ETAA1_31530(crtP)

TSPH:

KIH39_01650(crtI)

PBOR:

BSF38_05169(crtP)

AGV:	OJF2_67080(crtP)

PHM:	PSMK_03760(crtI)

MCAD:

Pan265_06390(crtP)

HBS:	IPV69_01380(crtI)

SFC:	Spiaf_2800

SLR:	L21SP2_2163

ACA:

ACP_2301

ACM:	AciX9_0858

TSA:	AciPR4_3647

TRS:	Terro_0346

ABAS:

ACPOL_3674

ORP:	MOP44_13920(crtI)

CTM:	Cabther_A1900

CVL:	J8C06_02330(crtI)

CHLO:

J8C02_09335(crtI)

DYS:	G7050_08290(crtI)

DYH:	G7051_08925(crtI)

BLQ:	L21SP5_01096(crtP)

ANF:

AQPE_1617

ASX:	CDL62_12200

PGIN:

FRZ67_12085(crtI)

FLS:	GLV81_02010(crtI)

LACS:

H4075_02190(crtI)

FLP:	LK994_03540(crtI)

HHY:	Halhy_4788

SGN:

SGRA_1067

PEJ:	FYC62_10480(crtI)

PEK:	FFJ24_025770(crtI)

PROE:

H9L23_26270(crtI)

PEX:	IZT61_17310(crtI)

MUP:	A0256_02300

MUH:	HYN43_029955(crtI)

MGK:	FSB76_11385(crtI)

MRUB:

DEO27_031165(crtI)

MGOS:

DIU38_019705(crtI)

MGIK:

GO620_013835(crtI)

MDJ:	LLH06_01120(crtI)

MSAB:

SNE25_31705(crtD)

SBX:	CA265_25315

CMR:	Cycma_1319

CAMU:

CA2015_1241

BBD:	Belba_0942

ALM:	AO498_02085

ALGO:

GYM62_18015(crtI)

MDA:	IPZ59_07745(crtI)

SMON:

AWR27_12515

SPIB:

G8759_08155(crtI)

STAE:

HNV11_05550(crtI)

SOQ:	LQ777_20265(crtI)

SENF:

GJR95_21410(crtI)

RHOZ:

GXP67_14360(crtI)

PSEZ:

HME7025_02380(crtI)

DSN:	HWI92_11400(crtI)

DYC:	NFI80_00625(crtI)

DFQ:	NFI81_02710(crtI)

RSI:	Runsl_1846

RUN:	DR864_18110

RUP:	DTQ70_22340(crtI)

EOL:	Emtol_2657

FAE:	FAES_0082(crtI)

FIB:	A6C57_24075

FLI:	Fleli_1387

HSW:

Hsw_4190

HYM:	N008_08365

HYD:	PK28_01500

HYE:	AM218_14535

HYG:	AUC43_05160

HYP:	A0257_15235

HYZ:	AXW84_19935

HNV:	DDQ68_14485

HYH:	D3Y59_13295(crtI)

HYJ:	FHG12_08555(crtI)

HRS:	HER32_10695(crtI)

HBN:	GUY19_13355(crtI)

HTS:	HMJ29_07230(crtI)

HMT:	MTP16_05475(crtI)

HTB:	MTX78_22945(crtI)

HYK:	O9Z63_17145(crtI)

HSK:	H4317_00310(crtI)

HCW:	O3303_15035(crtI)

HSB:	MWH26_17935(crtI)

HAEI:

MUN82_17145(crtI)

HCF:	MUN80_04940(crtI)

PKO:	PKOR_06065

PACT:

CA264_17000

RUF:	TH63_17895

RTI:	DC20_08645

RUD:	TH61_06260

NIB:	GU926_12040(crtI)

ADD:	HUW48_24355(crtI)

ASWU:

HUW51_08650(crtI)

MTT:	Ftrac_1976

MHAA:

Q3Y49_06840(crtI)

FPF:	DCC35_05045

FLM:	MY04_1513(crtI)

FLL:	EI427_04945(crtI)

FYA:	KMW28_08635(crtI)

FKA:	KM029_05570(crtI)

CLIT:

OQ292_03685(crtI)

IRO:	RT717_22295(crtD)

FBT:	D770_12865

FUV:	JR347_10465(crtI)

TPEL:

P0M28_13935(crtI)

RSR:	T7867_03195(crtD)

PPSV:

PEPS_03600

MARE:

EJ994_07520(crtI)

ZLA:	Q5W13_18300(crtI)

PTQ:	P700755_000615

PSYD:

IMZ30_02350(crtI)

NDO:

DDD_2394

NOM:	AAT17_12185

NOB:	CW736_08190

NOJ:	EJ995_11890(crtI)

NUL:	R1T42_07590(crtD)

NMF:

NMS_0121

POM:	MED152_02575(crtI1)

SYI:	SB49_01450

AQB:	D1818_13185(crtI)

AQA:	D1815_18000(crtI)

AQD:	D1816_14625(crtI)

AQE:	NBT05_05415(crtI)

OCI:	FEZ18_11935(crtI)

CAGG:

HYG79_01990(crtI)

ASAG:

FGM00_06240(crtI)

FLG:	LV716_01735(crtI)

SPON:

HME9304_02555(crtI)

FTE:	Fluta_0097

FLU:	CHH17_11240

OHO:	Oweho_0231

CHYD:

H4K34_00090(crtI)

BBAU:

AEM51_00705

CTS:

Ctha_0974

IAL:	IALB_0396(crtI)

CPRV:

CYPRO_2658

MEAM:

MU439_03715

MEAE:

QEN48_06670(crtI)

» show all

Reference

1 [PMID:15850982]

Authors

Mijts BN, Lee PC, Schmidt-Dannert C

Title

Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution.

Journal

Chem Biol 12:453-60 (2005)
DOI:10.1016/j.chembiol.2005.02.010

Sequence

[sav:SAV2564]

Reference

2 [PMID:15933032]

Authors

Tao L, Schenzle A, Odom JM, Cheng Q

Title

Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde.

Journal

Appl Environ Microbiol 71:3294-301 (2005)
DOI:10.1128/AEM.71.6.3294-3301.2005

Sequence

[sao:SAOUHSC_02881]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.44

IUBMB Enzyme Nomenclature:

1.14.99.44

ExPASy - ENZYME nomenclature database:

1.14.99.44

BRENDA, the Enzyme Database:

1.14.99.44

DBGET integrated database retrieval system