KEGG ENZYME: 1.14.99.54

ENZYME: 1.14.99.54

Entry

EC 1.14.99.54               Enzyme

Name

lytic cellulose monooxygenase (C1-hydroxylating);
lytic polysaccharide monooxygenase (ambiguous);
LPMO (ambiguous);
LPMO9A

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

cellulose, hydrogen-donor:oxygen oxidoreductase (D-glucosyl C1-hydroxylating)

Reaction(IUBMB)

[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = [(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone + [(1->4)-beta-D-glucosyl]n + acceptor + H2O [RN:R11745]

Reaction(KEGG)

R11745

Substrate

[(1->4)-beta-D-glucosyl]n+m;
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

[(1->4)-beta-D-glucosyl]m-1-(1->4)-D-glucono-1,5-lactone;
[(1->4)-beta-D-glucosyl]n;
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

This copper-containing enzyme, found in fungi and bacteria, cleaves cellulose in an oxidative manner. The cellulose fragments that are formed contain a D-glucono-1,5-lactone residue at the reducing end, which hydrolyses quickly and spontaneously to the aldonic acid. The electrons are provided in vivo by the cytochrome b domain of EC 1.1.99.18, cellobiose dehydrogenase (acceptor) [1]. Ascorbate can serve as the electron donor in vitro.

History

EC 1.14.99.54 created 2017

Orthology

K19356

lytic cellulose monooxygenase (C1-hydroxylating)

Genes

NCR:	NCU01867(gh61-10) NCU02240(gh61-1) NCU02916(gh61-3) NCU08760(gh61-5)

NTE:	NEUTE1DRAFT133749(NEUTE1DRAFT_133749) NEUTE1DRAFT82948(NEUTE1DRAFT_82948) NEUTE1DRAFT92381(NEUTE1DRAFT_92381)

SMP:	SMAC_00529 SMAC_03679 SMAC_04518 SMAC_06537 SMAC_06847

PAN:	PODANSg1734 PODANSg1847 PODANSg2226 PODANSg3538 PODANSg3552 PODANSg4671 PODANSg4737 PODANSg716 PODANSg7957 PODANSg8537

PBEL:

QC761_0025180 QC761_121900 QC761_201700 QC761_207120 QC761_401020 QC761_506950 QC761_508940 QC761_509980 QC761_703160 QC761_703770

PPSD:

QC762_0022440 QC762_121900 QC762_201700 QC762_207120 QC762_401020 QC762_506950 QC762_508940 QC762_509980 QC762_703160 QC762_703770

PPSP:

QC763_0025900 QC763_0102120 QC763_121900 QC763_201700 QC763_207120 QC763_401020 QC763_506950 QC763_508940 QC763_509980 QC763_703770

PPSA:

QC764_0025810 QC764_0111520 QC764_121900 QC764_201700 QC764_207120 QC764_401020 QC764_506950 QC764_508940 QC764_509980 QC764_703770

TTT:	THITE_2106556 THITE_2119040 THITE_2142696 THITE_2170662

MTM:	MYCTH_111088 MYCTH_2299721 MYCTH_2301632 MYCTH_47093 MYCTH_79765 MYCTH_80312

CTHR:

CTHT_0000930 CTHT_0022130 CTHT_0052310 CTHT_0072140

MGR:	MGG_07300 MGG_08020 MGG_12733

PPEI:

PpBr36_01463 PpBr36_01943 PpBr36_06416

PGRI:

PgNI_00792 PgNI_03554 PgNI_05405 PgNI_07727

TMN:	UCRPA7_2324 UCRPA7_5174 UCRPA7_5653 UCRPA7_5990

SSCK:

SPSK_01239 SPSK_01887 SPSK_04252 SPSK_09715

FGR:	FGSG_03695 FGSG_04773 FGSG_06397

FPU:	FPSE_00868 FPSE_01837 FPSE_02468 FPSE_07781 FPSE_12300

FPOA:

FPOAC1_006095 FPOAC1_009394

FVN:	FVRRES_06695 FVRRES_09706 FVRRES_10031

FVR:	FVEG_03506 FVEG_04743 FVEG_06459 FVEG_08783

FOX:	FOXG_05654 FOXG_07821 FOXG_08211 FOXG_09688

NHE:	NECHADRAFT_100325 NECHADRAFT_106448 NECHADRAFT_19032 NECHADRAFT_33435 NECHADRAFT_96284

FFC:	NCS54_00283300 NCS54_01304900

FKR:	NCS57_00278600 NCS57_01291600

FMU:	J7337_004332 J7337_006778 J7337_012544

TRE:	TRIREDRAFT_120961(cel61b)

TRR:	M419DRAFT_122518

VAL:	VDBG_01593 VDBG_02240 VDBG_03308 VDBG_07467 VDBG_08426 VDBG_09242 VDBG_09562

VDA:	VDAG_01959 VDAG_02211 VDAG_02299 VDAG_02711 VDAG_02959 VDAG_03411 VDAG_04756 VDAG_04795 VDAG_07400 VDAG_08091

CFJ:	CFIO01_02234 CFIO01_03891 CFIO01_04309 CFIO01_04911 CFIO01_05808 CFIO01_10036 CFIO01_10898

CLUP:

CLUP02_04841 CLUP02_11571 CLUP02_16314 CLUP02_16898

CHIG:

CH63R_00346 CH63R_06721 CH63R_09615 CH63R_10341 CH63R_10722 CH63R_11040 CH63R_12595

SAPO:

SAPIO_CDS10182 SAPIO_CDS1237 SAPIO_CDS1238 SAPIO_CDS1536 SAPIO_CDS6278 SAPIO_CDS8458 SAPIO_CDS9232

ELA:	UCREL1_10876 UCREL1_1520 UCREL1_2340 UCREL1_312 UCREL1_319 UCREL1_3268 UCREL1_3819 UCREL1_4406 UCREL1_4875 UCREL1_5189 UCREL1_6396 UCREL1_6408 UCREL1_9067

PFY:	PFICI_01743 PFICI_03695 PFICI_04173 PFICI_08510 PFICI_10057 PFICI_12594 PFICI_14484 PFICI_15159 PFICI_15210

SSL:	SS1G_09251 SS1G_12059

BFU:	BCIN_03g05890 BCIN_05g08230 BCIN_06g00480

MBE:	MBM_00712 MBM_04929 MBM_06977

PSCO:

LY89DRAFT_582205 LY89DRAFT_634019 LY89DRAFT_760760 LY89DRAFT_772659

GLZ:	GLAREA_00867 GLAREA_02347 GLAREA_04229 GLAREA_08312 GLAREA_08367 GLAREA_09224 GLAREA_11571 GLAREA_12658

ANI:	ANIA_01602

AFM:	AFUA_8G06830

ACT:	ACLA_059790

NFI:	NFIA_099510

AOR:	AO090005000531

ANG:	An15g04900

AFV:	AFLA_003339

ALUC:

AKAW2_60257S

APUU:

APUU_21609A

PNO:	SNOG_00879 SNOG_01146 SNOG_01922 SNOG_05441 SNOG_05737 SNOG_05750 SNOG_07503 SNOG_10071 SNOG_12037 SNOG_14481

PTE:	PTT_04452 PTT_08333 PTT_09156 PTT_10713 PTT_12296 PTT_12378 PTT_12389 PTT_12419 PTT_13147 PTT_14450 PTT_19839

BZE:	COCCADRAFT_10290 COCCADRAFT_27639 COCCADRAFT_37868 COCCADRAFT_80996 COCCADRAFT_81058 COCCADRAFT_82910 COCCADRAFT_8654 COCCADRAFT_88798 COCCADRAFT_89549 COCCADRAFT_90790 COCCADRAFT_91341 COCCADRAFT_97778

BSC:	COCSADRAFT_141473 COCSADRAFT_163931 COCSADRAFT_173335 COCSADRAFT_184615 COCSADRAFT_217864 COCSADRAFT_296363 COCSADRAFT_32038 COCSADRAFT_33583 COCSADRAFT_33682 COCSADRAFT_34597 COCSADRAFT_36666 COCSADRAFT_41104

BOR:	COCMIDRAFT_100168 COCMIDRAFT_102803 COCMIDRAFT_24605 COCMIDRAFT_25375 COCMIDRAFT_35579 COCMIDRAFT_36538 COCMIDRAFT_36916 COCMIDRAFT_5637 COCMIDRAFT_85853 COCMIDRAFT_87449 COCMIDRAFT_895 COCMIDRAFT_90535 COCMIDRAFT_99299

AALT:

CC77DRAFT_1005473 CC77DRAFT_1006432 CC77DRAFT_1019743 CC77DRAFT_1028092 CC77DRAFT_1044591 CC77DRAFT_1056084 CC77DRAFT_1057322 CC77DRAFT_190672 CC77DRAFT_307404 CC77DRAFT_504833 CC77DRAFT_705864 CC77DRAFT_937160 CC77DRAFT_940430 CC77DRAFT_941177 CC77DRAFT_948935

ARAB:

EKO05_0003290 EKO05_0003463 EKO05_0006168 EKO05_0008432 EKO05_0008862 EKO05_0009560 EKO05_0009638 EKO05_0010429

NPA:	UCRNP2_10454 UCRNP2_3883 UCRNP2_5067

TML:	GSTUM_00001211001

TVS:	TRAVEDRAFT_143379 TRAVEDRAFT_147827 TRAVEDRAFT_162601 TRAVEDRAFT_51004 TRAVEDRAFT_71674

DSQ:	DICSQDRAFT_102982 DICSQDRAFT_104462 DICSQDRAFT_137375 DICSQDRAFT_181042

PCO:	PHACADRAFT_251256 PHACADRAFT_253391 PHACADRAFT_261448 PHACADRAFT_263097 PHACADRAFT_57963

SHS:	STEHIDRAFT_105725 STEHIDRAFT_121343 STEHIDRAFT_98247

HIR:	HETIRDRAFT_63659(GH61H) HETIRDRAFT_64994(GH61I) HETIRDRAFT_67582(GH61D)

PSQ:	PUNSTDRAFT_142953 PUNSTDRAFT_154782 PUNSTDRAFT_154784 PUNSTDRAFT_154785 PUNSTDRAFT_96583

ADL:	AURDEDRAFT_132370 AURDEDRAFT_140513 AURDEDRAFT_143034 AURDEDRAFT_165835 AURDEDRAFT_199593 AURDEDRAFT_86786

FME:	FOMMEDRAFT_136574 FOMMEDRAFT_170904 FOMMEDRAFT_187182 FOMMEDRAFT_38849

RSX:	RhiXN_04074 RhiXN_05310 RhiXN_07972 RhiXN_07974 RhiXN_09310 RhiXN_09321 RhiXN_09329 RhiXN_09382 RhiXN_11095 RhiXN_12126

CCI:	CC1G_02189 CC1G_03525 CC1G_03526 CC1G_03529 CC1G_04917 CC1G_07179 CC1G_07480 CC1G_09526 CC1G_09949 CC1G_10029 CC1G_13046

ABP:	AGABI1DRAFT111761(AGABI1DRAFT_111761) AGABI1DRAFT113096(AGABI1DRAFT_113096) AGABI1DRAFT80328(AGABI1DRAFT_80328)

ABV:	AGABI2DRAFT122757(AGABI2DRAFT_122757) AGABI2DRAFT192993(AGABI2DRAFT_192993) AGABI2DRAFT226235(AGABI2DRAFT_226235)

PCUB:

JR316_0002821 JR316_0007184 JR316_0008092 JR316_0011395 JR316_0011805 JR316_0011856

MPR:	MPER_08960 MPER_10540

MRR:	Moror_11015 Moror_12493 Moror_12809 Moror_14820 Moror_16033 Moror_16034

E1B28_004841 E1B28_005688 E1B28_008037 E1B28_008051 E1B28_009655(CEL1) E1B28_013585 E1B28_013836

SCM:	SCHCO_01280218(SCHCODRAFT_01280218) SCHCO_02076751(SCHCODRAFT_02076751) SCHCO_02621099(SCHCODRAFT_02621099) SCHCO_02640531(SCHCODRAFT_02640531)

CPUT:

CONPUDRAFT_140516 CONPUDRAFT_91873

SLA:	SERLADRAFT_335267

WSE:	WALSEDRAFT_32834

WIC:	J056_000100 J056_002693

» show all

Reference

1 [PMID:22004347]

Authors

Phillips CM, Beeson WT, Cate JH, Marletta MA

Title

Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa.

Journal

ACS Chem Biol 6:1399-406 (2011)
DOI:10.1021/cb200351y

Sequence

[ncr:NCU08760]

Reference

2 [PMID:22188218]

Authors

Beeson WT, Phillips CM, Cate JH, Marletta MA

Title

Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases.

Journal

J Am Chem Soc 134:890-2 (2012)
DOI:10.1021/ja210657t

Sequence

[ncr:NCU08760]

Reference

3 [PMID:22578542]

Authors

Li X, Beeson WT 4th, Phillips CM, Marletta MA, Cate JH

Title

Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases.

Journal

Structure 20:1051-61 (2012)
DOI:10.1016/j.str.2012.04.002

Reference

4 [PMID:23124232]

Authors

Bey M, Zhou S, Poidevin L, Henrissat B, Coutinho PM, Berrin JG, Sigoillot JC

Title

Cello-oligosaccharide oxidation reveals differences between two lytic polysaccharide monooxygenases (family GH61) from Podospora anserina.

Journal

Appl Environ Microbiol 79:488-96 (2013)
DOI:10.1128/AEM.02942-12

Sequence

[pan:PODANSg8471 PODANSg4737]

Reference

5 [PMID:26185526]

Authors

Frommhagen M, Sforza S, Westphal AH, Visser J, Hinz SW, Koetsier MJ, van Berkel WJ, Gruppen H, Kabel MA

Title

Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase.

Journal

Biotechnol Biofuels 8:101 (2015)
DOI:10.1186/s13068-015-0284-1

Reference

6 [PMID:27213015]

Authors

Patel I, Kracher D, Ma S, Garajova S, Haon M, Faulds CB, Berrin JG, Ludwig R, Record E

Title

Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6.

Journal

Biotechnol Biofuels 9:108 (2016)
DOI:10.1186/s13068-016-0520-3

Reference

7 [PMID:27152023]

Authors

Courtade G, Wimmer R, Rohr AK, Preims M, Felice AK, Dimarogona M, Vaaje-Kolstad G, Sorlie M, Sandgren M, Ludwig R, Eijsink VG, Aachmann FL

Title

Interactions of a fungal lytic polysaccharide monooxygenase with beta-glucan substrates and cellobiose dehydrogenase.

Journal

Proc Natl Acad Sci U S A 113:5922-7 (2016)
DOI:10.1073/pnas.1602566113

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.54

IUBMB Enzyme Nomenclature:

1.14.99.54

ExPASy - ENZYME nomenclature database:

1.14.99.54

BRENDA, the Enzyme Database:

1.14.99.54

DBGET integrated database retrieval system