KEGG ENZYME: 1.17.5.3

ENZYME: 1.17.5.3

Entry

EC 1.17.5.3                 Enzyme

Name

formate dehydrogenase-N;
Fdh-N;
FdnGHI;
nitrate-inducible formate dehydrogenase;
formate dehydrogenase N;
FDH-N;
nitrate inducible Fdn;
nitrate inducible formate dehydrogenase

Class

Oxidoreductases;
Acting on CH or CH2 groups;
With a quinone or similar compound as acceptor

Sysname

formate:quinone oxidoreductase

Reaction(IUBMB)

formate + a quinone = CO2 + a quinol [RN:R09494]

Reaction(KEGG)

R09494

Substrate

formate [CPD:C00058];
quinone [CPD:C00472]

Product

CO2 [CPD:C00011];
quinol [CPD:C00530]

Comment

The enzyme contains molybdopterin-guanine dinucleotides, five [4Fe-4S] clusters and two heme b groups. Formate dehydrogenase-N oxidizes formate in the periplasm, transferring electrons via the menaquinone pool in the cytoplasmic membrane to a dissimilatory nitrate reductase (EC 1.7.5.1), which transfers electrons to nitrate in the cytoplasm. The system generates proton motive force under anaerobic conditions [3].

History

EC 1.17.5.3 created 2010 as EC 1.1.5.6, transferred 2017 to EC 1.17.5.3

Orthology

K08348

formate dehydrogenase-N, alpha subunit

Genes

ECO:	b1474(fdnG)

ECJ:	JW1470(fdnG)

ECD:	ECDH10B_1605(fdnG)

EBW:	BWG_1295(fdnG)

ECOK:

ECMDS42_1186(fdnG)

ECOC:

C3026_08550

ECE:	Z2236(fdnG)

ECS:	ECs_2078(fdnG)

EOI:	ECO111_1864(fdnG)

EOJ:	ECO26_2072(fdnG)

EOH:	ECO103_1603(fdnG)

ECOO:

ECRM13514_1928(fdnG)

ECOH:

ECRM13516_1830(fdnG)

ECK:	EC55989_1605(fdnG)

ECG:	E2348C_1608(fdnG)

ELR:	ECO55CA74_08985 ECO55CA74_08990

ELH:

ETEC_1545

ECW:	EcE24377A_1655(fdnG)

EUN:	UMNK88_1878 UMNK88_1879

ECP:	ECP_1474 ECP_1475

ENA:	ECNA114_3647 ECNA114_36471

ECOS:

EC958_1738 EC958_1739

ECV:	APECO1_609(fdnG)

ECOA:

APECO78_11090 APECO78_11095

ECX:	EcHS_A1557(fdnG)

ECM:	EcSMS35_1700(fdnG)

ECY:	ECSE_1562 ECSE_1563

ECR:	ECIAI1_1477(fdnG)

ECQ:	ECED1_1624(fdnG)

EUM:	ECUMN_1727(fdnG)

ECT:	ECIAI39_1736(fdnG)

EOC:	CE10_1663(fdnG)

EBR:	ECB_01432(fdnG)

ECOB:

C3029_11745

EBL:	ECD_01432(fdnG)

EBE:	B21_01444(fdnG)

EBD:

ECBD_2165

ECI:	UTI89_C1689(fdnG)

EIH:	ECOK1_1631(fdnG)

ECZ:	ECS88_1566(fdnG)

ECC:	c5623(fdnG)

ELO:	EC042_1604(fdnG)

ELN:	NRG857_07300 NRG857_07305

ESE:	ECSF_1387 ECSF_1388

ECL:	EcolC_2183

EKO:	EKO11_2346 EKO11_2347

EAB:	ECABU_c17080(fdnG)

EDH:	EcDH1_2173 EcDH1_2174

EDJ:	ECDH1ME8569_1417(fdnG)

ELU:	UM146_09665 UM146_09670

ELW:	ECW_m1602(fdnG)

ELL:	WFL_07835(fdnG1)

ELP:	P12B_c1654(fdnG) P12B_c1655(fdnG)

ELF:	LF82_0630(fdnG)

ECOL:

LY180_07640 LY180_07645

ECOJ:

P423_08190 P423_08195

EFE:	EFER_1489(fdnG)

EAL:	EAKF1_ch0027c EAKF1_ch0028c

EMA:	C1192_04735 C1192_04740(fdnG)

ESZ:	FEM44_22465(fdnG)

ERUY:

OSH18_02355(fdnG)

SEX:	STBHUCCB_15800

SENT:

TY21A_07540

STM:	STM1570(fdnG)

SEO:	STM14_1894(fdnG)

SEV:	STMMW_15651

SEY:	SL1344_1500(fdnG)

SEM:	STMDT12_C15890

SEB:	STM474_1583(fdnG)

SEF:	UMN798_1644(fdnG) UMN798_1645(fdnG)

SETC:

CFSAN001921_09250 CFSAN001921_09255

SENR:

STMDT2_14931(fdnG)

SENI:

CY43_08015 CY43_08020

SEEN:

SE451236_13755 SE451236_13760

SPT:	SPA1298(fdnG)

SEK:

SSPA1206

SENH:

CFSAN002069_01115 CFSAN002069_01120

SENN:

SN31241_26440 SN31241_26450

SEG:	SG1557(fdnG)

SET:	SEN1485(fdnG)

SENA:

AU38_07665 AU38_07670

SENO:

AU37_07665 AU37_07670

SENV:

AU39_07675 AU39_07680

SENQ:

AU40_08610 AU40_08615

SENL:

IY59_07860 IY59_07865

SEEC:

CFSAN002050_14245 CFSAN002050_14250

SEEB:

SEEB0189_011660 SEEB0189_011665

SENB:

BN855_16160(fdnG)

SENE:

IA1_07770 IA1_07775

SENC:

SEET0819_14540 SEET0819_14545

SBZ:	A464_1593 A464_1595

SBV:	N643_06690 N643_06695

SALZ:

EOS98_11545 EOS98_11550(fdnG)

SFL:	SF1751(fdnG)

SFX:	S1884(fdnG)

SFV:	SFV_1747(fdnG)

SFN:	SFy_2508 SFy_2509

SFS:	SFyv_2563 SFyv_2564

SSN:	SSON_1650(fdnG)

SBO:	SBO_1583(fdnG)

SDZ:	Asd1617_02149 Asd1617_02150 Asd1617_02152 Asd1617_02153

SHQ:	A0259_11415 A0259_11420

ENC:	ECL_01901 ECL_01902

ENL:	A3UG_11755 A3UG_11760

ECLG:

EC036_22400 EC036_22410

ECLE:

ECNIH2_11870 ECNIH2_11875

ECLN:

ECNIH4_11325 ECNIH4_11330

ECLI:

ECNIH5_10795 ECNIH5_10800

ECLX:

LI66_11170 LI66_11175

ECLY:

LI62_13315 LI62_13320

ECLZ:

LI64_10985 LI64_10990

ECLO:

ENC_09820

EHM:	AB284_13745 AB284_13750

EXF:	BFV63_11425 BFV63_11430

ECLA:

ECNIH3_11020 ECNIH3_11025

ECLC:

ECR091_10960 ECR091_10965

EAU:	DI57_07230 DI57_07235

EKB:	BFV64_11795 BFV64_11800

ENO:	ECENHK_11435 ECENHK_11440

EEC:	EcWSU1_02353(fdnG) EcWSU1_02354(fdnG)

ELG:	BH714_07740 BH714_07745

ECAN:

CWI88_11350 CWI88_11355(fdnG)

ERN:	BFV67_11215 BFV67_11220

ECLS:

LI67_014150

ECHG:

FY206_12940(fdnG)

ESH:	C1N69_11665(fdnG)

EPT:	HWQ17_01470(fdnG)

ENT:	Ent638_1750 Ent638_2054 Ent638_2055

EAS:	Entas_1974 Entas_2221 Entas_2222

ENR:	H650_02770 H650_02775 H650_03170

ENX:	NI40_011650 NI40_011655

ENF:	AKI40_3158(fdnG) AKI40_3159

LNI:	CWR52_00620 CWR52_00625(fdnG)

EBG:	FAI37_17690(fdnG)

END:	A4308_16115 A4308_16120

ENZ:	G0034_11585(fdnG)

ENS:	HWQ15_19890(fdnG)

ENK:	LOC22_21610(fdnG)

EHU:	D5067_0011355(fdnG)

EMOR:

L6Y89_11245(fdnG)

ENB:	ELK40_11890(fdnG)

EQU:	OM418_11565(fdnG)

EPU:	QVH39_12195(fdnG)

KPN:	KPN_01867(fdnG)

KPU:	KP1_2931(fdnG)

KPM:	KPHS_28360 KPHS_28370

KPP:

A79E_2378

KPH:	KPNIH24_14220 KPNIH24_14225

KPZ:	KPNIH27_13555 KPNIH27_13560

KPV:	KPNIH29_14225 KPNIH29_14230

KPW:	KPNIH30_14490 KPNIH30_14495

KPY:	KPNIH31_13505 KPNIH31_13510

KPG:	KPNIH32_14385 KPNIH32_14390

KPC:	KPNIH10_14025 KPNIH10_14030

KPQ:	KPR0928_14005 KPR0928_14010

KPT:	VK055_0616 VK055_0617

KPO:	KPN2242_12250 KPN2242_12255

KPR:	KPR_2929(fdnG)

KPI:	D364_09570 D364_09575

KPA:	KPNJ1_02610 KPNJ1_02611

KPS:	KPNJ2_02566 KPNJ2_02567

KPX:	PMK1_04207(fdnG_1) PMK1_04208(fdnG_2)

KPB:	FH42_05405 FH42_05410

KPNE:

KU54_012125 KU54_012130

KPNU:

LI86_12060 LI86_12065

KPNK:

BN49_2967(fdnG)

KVA:	Kvar_2437 Kvar_2438

KPE:	KPK_2489(fdnG)

KPK:	A593_24195 A593_24200

KVD:	KR75_22130 KR75_22135

KVQ:	SP68_02875 SP68_02880

KOX:	KOX_20025 KOX_20030

KOE:

A225_2876

KOY:	J415_17595 J415_17600

KOM:	HR38_18355 HR38_18360

KMI:	VW41_05125 VW41_05130

KOK:	KONIH1_14090 KONIH1_14095

KOC:	AB185_22250 AB185_22255

KQU:	AVR78_18365 AVR78_18370

EAE:	EAE_19865 EAE_19870

EAR:	CCG29906 CCG29907

KQV:	B8P98_13170 B8P98_13175(fdnG)

KLL:	BJF97_14255 BJF97_14260

KLW:	DA718_14030 DA718_14035(fdnG)

KAR:	LGL98_11665(fdnG)

KGR:	JJJ10_09175(fdnG) JJJ10_14625(fdnG)

KPAS:

LUW96_20560(fdnG) LUW96_25815(fdnG)

KLC:	K7H21_09245(fdnG) K7H21_13770(fdnG)

KLM:	BWI76_13740 BWI76_19155

REE:	electrica_02598(fdnG_1) electrica_02600(fdnG_2) electrica_04112(fdnG_3)

ROR:	RORB6_06740 RORB6_06745

RON:	TE10_13840 TE10_13845

RPLN:

B1209_13840 B1209_13845

RAO:	DSD31_13360(fdnG)

RTG:	NCTC13098_06023(fdnG_1)

CRO:	ROD_15621(fdnG)

CFD:	CFNIH1_17935 CFNIH1_17940

CBRA:

A6J81_04390(fdnG) A6J81_04780(fdnG)

CWE:	CO701_10300 CO701_10305(fdnG)

CYO:	CD187_11765 CD187_11770(fdnG)

CFQ:	C2U38_12040 C2U38_12045(fdnG)

CSED:

JY391_11140(fdnG)

CAMA:

F384_07195 F384_07200

CAF:	AL524_15110 AL524_15115(fdnG)

CIF:	AL515_12255(fdnG)

CFAR:

CI104_13145(fdnG)

CIR:	C2U53_22215(fdnG)

CIE:	AN232_17870 AN232_17875(fdnG)

CPAR:

CUC49_07970(fdnG)

CTEL:

GBC03_13400(fdnG) GBC03_17345(fdnG)

CITZ:

E4Z61_05145(fdnG)

CARS:

E1B03_13415(fdnG)

CIX:	M4I31_11620(fdnG)

CIB:	HF677_011800(fdnG)

CENS:

P2W74_12040(fdnG)

EBT:	EBL_c27500 EBL_c27510

CNT:	JT31_07180 JT31_07185

CEM:	LH23_10550 LH23_10555

CEN:	LH86_10570 LH86_10575

PGE:	LG71_17210 LG71_17215

ESC:	Entcl_2349 Entcl_2350

KLE:	AO703_10480 AO703_10485

KSA:	C813_03400 C813_03775 C813_24500

KOR:	AWR26_12790(fdnG)

KRD:	A3780_11255 A3780_11260

KCO:	BWI95_16120 BWI95_16125

KOT:	EH164_11525(fdnG) EH164_11845(fdnG)

KPSE:

IP581_11315(fdnG)

KOB:	HF650_11290(fdnG)

KOO:	O9K67_12410(fdnG)

KAS:	KATP_22420 KATP_22430

KLU:	K7B04_17345(fdnG)

KCY:	RIN60_11540(fdnG) RIN60_18115(fdnG)

LAX:	APT61_11090 APT61_11095

LEI:	C2U54_16530(fdnG)

LEH:	C3F35_00250 C3F35_00255(fdnG)

LEE:	DVA44_11955

LER:	GNG29_11045(fdnG)

LEA:	GNG26_11310(fdnG)

LPNU:

KQ929_10100(fdnG)

LEY:	DVA43_06235 DVA43_06240(fdnG)

LAZ:	A8A57_11095

LEF:	LJPFL01_1786 LJPFL01_2133 LJPFL01_2134

LEW:	DAI21_15460 DAI21_15465(fdnG)

BAGE:

BADSM9389_30600 BADSM9389_30610

AHN:	NCTC12129_00262(fdnG_2) NCTC12129_03677(fdnG_3)

ASUB:

NLZ15_00990(fdnG)

YRE:	HEC60_05625(fdnG)

KIN:	AB182_10840 AB182_12330 AB182_12335

PDZ:	HHA33_13540(fdnG) HHA33_15130(fdnG)

METY:

MRY16398_24300(fdnG) MRY16398_29020 MRY16398_29030

IZH:	FEM41_12715(fdnG)

PSGC:

G163CM_11680(fdnG_2)

SUPE:

P0H77_10970(fdnG)

TOE:	QMG90_11105(fdnG)

EBF:	D782_2135 D782_2136

EBC:	C2U52_21525(fdnG) C2U52_23225(fdnG)

EBU:	CUC76_01790 CUC76_01795(fdnG)

EBB:

F652_4113

SFW:	WN53_07410 WN53_07415

SFG:	AV650_03405 AV650_03410

SFO:	Z042_26160

RAH:	Rahaq_0044

RACE:

JHW33_16845(fdnG)

RAQ:	Rahaq2_0050

RAA:

Q7S_00210

ROX:	BV494_16450

RVC:	J9880_08000(fdnG)

RBON:

QNM34_00190(fdnG)

RIU:	I2123_02245(fdnG)

GQU:	AWC35_19195

ECA:	ECA1408(fdnG)

PATR:

EV46_07130

PATO:

GZ59_14450(fdnG)

PCT:

PC1_1286

PCC:	PCC21_013180

PCV:	BCS7_06600

PWA:	Pecwa_3045

PPAR:

A8F97_03470

PEC:

W5S_3034

PWS:	A7983_15605

PPOA:

BJK05_01640

PBRA:

B5S52_15075

PPUJ:

E2566_07135(fdnG)

PARI:

I2D83_15565(fdnG)

PAQU:

DMB82_0013955(fdnG)

PVZ:	OA04_14900(fdnG)

PPAV:

LOZ86_06980(fdnG)

PQU:	IG609_013720(fdnG)

PPOO:

LW347_07075(fdnG)

PCAC:

OI450_06375(fdnG)

PACB:

M9782_09030(fdnG)

DDD:	Dda3937_02963(fdnG)

DZE:	Dd1591_2662

DDC:	Dd586_2558

DZC:	W909_07530

DSO:	A4U42_01755 A4U42_16865

CED:	LH89_04310 LH89_19930

DFN:	CVE23_08490(fdnG)

DDQ:

DDI_1292

DAQ:	DAQ1742_01538(fdnG)

DIC:	Dpoa569_002332(fdnG)

DLC:	O1Q98_19190(fdnG)

DDA:	Dd703_1335

BGJ:	AWC36_11695

PAJ:	PAJ_0232(FdnG)

PVA:	Pvag_3327(fdnG)

PAGG:

AL522_04785(fdnG)

PANP:

PSNIH2_17870

PAGC:

BEE12_07160

PALH:

B1H58_06955

PANO:

OJ965_20465(fdnG)

KPIE:

N5580_00245(fdnG)

PALF:

K6R05_18300(fdnG)

PCD:	C2E16_00880(fdnG)

MINT:

C7M51_03641(fdnG)

MTHI:

C7M52_03392(fdnG)

MHAN:

K6958_00555(fdnG)

PAY:	PAU_00744(fdnG)

PTT:	VY86_04090

PAKH:

B0X70_04425

PLUI:

CE143_04355(fdnG)

PVG:	CRN77_04695(fdnG)

PHAU:

PH4a_06470(fdnG)

PROT:

BTA34_16235

PCOL:

F1325_18505(fdnG)

PCIB:

F9282_19075(fdnG)

PSI:	S70_17650 S70_17655

PSX:	DR96_2483 DR96_2484

PSTA:

BGK56_19885 BGK56_19890

PTHA:

OI982_05700(fdnG)

PRG:	RB151_014060(fdnG_1) RB151_014070(fdnG_2)

PALA:

CO695_16965(fdnG)

PHEI:

NCTC12003_00220(fdnG)

PRQ:	CYG50_09120(fdnG)

PVC:	G3341_13440(fdnG)

PMAG:

JI723_13350(fdnG)

PHAG:

PZ638_13670(fdnG)

MMK:	MU9_1138 MU9_1139

MWI:	MNY66_13670(fdnG)

EIC:	NT01EI_3713 NT01EI_3714

ETR:	ETAE_3337 ETAE_3338

ETD:	ETAF_3025 ETAF_3338

ETC:	ETAC_15930 ETAC_15935

HAV:	AT03_04270 AT03_04275

HPAR:

AL518_01330(fdnG)

OPO:	DSM2777_21905 DSM2777_21910

LPV:	HYN51_13205(fdnG)

PRAG:

EKN56_03610(fdnG)

PFQ:	QQ39_17380

ADP:	NCTC12871_00141(fdnG)

GSS:	NYR30_09145(fdnG)

PDS:	CAY62_02595 CAY62_02600

PMK:

MDS_4225

PPRC:

PFLCHA0_c32650(fdoG)

PPRO:

PPC_3259

PLQ:	AA042_20910

PALK:

PSAKL28_29570

PSW:	LK03_16505

AVL:	AvCA_03810(fdhA)

AVD:	AvCA6_03810(fdhA)

ACX:	Achr_37960(fdhA)

SON:	SO_0101(fdnG)

SBM:	Shew185_0099

SBP:	Sbal223_0104

SBT:	Sbal678_0108

SPL:

Spea_0437

SHM:	Shewmr7_0097

SHN:	Shewana3_2089

SHL:

Shal_0492

SBJ:	CF168_00535 CF168_00540(fdnG) CF168_10260(fdnG)

SHEW:

CKQ84_08700 CKQ84_08705(fdnG)

SALG:

BS332_04165

SDK:	KHX94_11780(fdnG)

SCAA:

TUM17387_33350(fdnG)

SEUR:

FM038_021825(fdnG)

SXM:	MKD32_00540(fdnG) MKD32_10145(fdnG)

SSEH:

N7V09_02085(fdnG)

SCHK:

GII14_02390(fdnG)

SDEO:

D0436_00710(fdnG) D0436_10910(fdnG)

SMAY:

K0H60_00735(fdnG) K0H60_10545(fdnG)

SOG:	RA178_00535(fdnG)

KUS:	B9G99_04270

WCN:	PE074_07705(fdnG)

NEI:	BG910_02450(fdnG)

NBL:	GJV52_02430(fdnG)

VFF:	VITFI_CDS0376

AQL:	BXU06_10390

RPJ:	N234_10235

CNC:	CNE_2c14340(fdoG)

CUH:	BJN34_31335

CTI:	RALTA_B1681(fdnG)

COX:	E0W60_02610(fdnG)

AFA:	UZ73_04120

ODI:

ODI_R1009

CKE:	B5M06_15395

SWS:	I6J16_07240

QAU:	KI612_01395(fdnG)

APK:	APA386B_352(fdnG)

ASZ:	ASN_3403(fdoG)

APER:

A0U91_00725

APOM:

CPF11_12735(fdnG)

ATO:	CIW82_14445(fdnG)

ACET:

DS739_07695(fdnG)

NEH:	E3E11_02405(fdnG)

SWF:	E3E12_02660(fdnG)

AZM:	DM194_14325(fdnG)

HET:	BBW65_03215

CJU:

C8J_1415

CJN:	ICDCCJ07001_1438

CJM:	CJM1_1456(fdhA)

CJQ:	UC78_1444(fdnG)

ABT:

ABED_1396

NAP:	C3L23_06860

GSK:	KN400_0757(fdnG)

GEM:

GM21_1955

GEB:

GM18_1962

GUR:

Gura_3388

GEO:	Geob_1634(fdnG-1)

GBM:	Gbem_2267(fdnG-1)

GBN:	GEOBRER4_21210(fdnG)

BDA:	FSZ17_22305(fdnG)

NMK:	CHR53_27385(fdnG)

AIA:	AWH56_013365(fdnG)

BBEV:

BBEV_0717(fdhA-1)

BSE:

Bsel_1218

SALQ:

SYNTR_0512

ATL:	Athai_16180

» show all

Reference

1 [PMID:1099093]

Authors

Enoch HG, Lester RL

Title

The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli.

Journal

J Biol Chem 250:6693-705 (1975)

Sequence

[eco:b1474 b1475 b1476]

Reference

2 [PMID:11884747]

Authors

Jormakka M, Tornroth S, Byrne B, Iwata S

Title

Molecular basis of proton motive force generation: structure of formate dehydrogenase-N.

Journal

Science 295:1863-8 (2002)
DOI:10.1126/science.1068186

Sequence

[eco:b1474 b1475 b1476]

Reference

3 [PMID:11752799]

Authors

Jormakka M, Tornroth S, Abramson J, Byrne B, Iwata S

Title

Purification and crystallization of the respiratory complex formate dehydrogenase-N from Escherichia coli.

Journal

Acta Crystallogr D Biol Crystallogr 58:160-2 (2002)
DOI:10.1107/S0907444901017723

Sequence

[eco:b1474 b1475 b1476]

Other DBs

ExplorEnz - The Enzyme Database:

1.17.5.3

IUBMB Enzyme Nomenclature:

1.17.5.3

ExPASy - ENZYME nomenclature database:

1.17.5.3

BRENDA, the Enzyme Database:

1.17.5.3

DBGET integrated database retrieval system