KEGG   ENZYME: 1.17.99.2
Entry
EC 1.17.99.2                Enzyme                                 
Name
ethylbenzene hydroxylase;
ethylbenzene dehydrogenase;
ethylbenzene:(acceptor) oxidoreductase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
Sysname
ethylbenzene:acceptor oxidoreductase
Reaction(IUBMB)
ethylbenzene + H2O + acceptor = (S)-1-phenylethanol + reduced acceptor [RN:R05745]
Reaction(KEGG)
R05745
Substrate
ethylbenzene [CPD:C07111];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Product
(S)-1-phenylethanol [CPD:C11348];
reduced acceptor [CPD:C00030]
Comment
Involved in the anaerobic catabolism of ethylbenzene by denitrifying bacteria. Ethylbenzene is the preferred substrate; the enzyme from some strains oxidizes propylbenzene, 1-ethyl-4-fluorobenzene, 3-methylpent-2-ene and ethylidenecyclohexane. Toluene is not oxidized. p-Benzoquinone or ferrocenium can act as electron acceptor. Contains molybdopterin, [4Fe-4S] clusters and heme b.
History
EC 1.17.99.2 created 2001
Pathway
ec00642  Ethylbenzene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10700  ethylbenzene hydroxylase subunit alpha
Genes
EBAc1A65(ebdA)
Reference
1  [PMID:11294876]
  Authors
Kniemeyer O, Heider J.
  Title
Ethylbenzene dehydrogenase, a novel hydrocarbon-oxidizing molybdenum/iron-sulfur/heme enzyme.
  Journal
J Biol Chem 276:21381-6 (2001)
DOI:10.1074/jbc.M101679200
Reference
2  [PMID:11443088]
  Authors
Johnson HA, Pelletier DA, Spormann AM
  Title
Isolation and characterization of anaerobic ethylbenzene dehydrogenase, a novel Mo-Fe-S enzyme.
  Journal
J Bacteriol 183:4536-42 (2001)
DOI:10.1128/JB.183.15.4536-4542.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.2
IUBMB Enzyme Nomenclature: 1.17.99.2
ExPASy - ENZYME nomenclature database: 1.17.99.2
UM-BBD (Biocatalysis/Biodegradation Database): 1.17.99.2
BRENDA, the Enzyme Database: 1.17.99.2
CAS: 372947-56-3

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