KEGG   ENZYME: 1.17.99.8
Entry
EC 1.17.99.8                Enzyme                                 
Name
limonene dehydrogenase;
ctmAB (gene names)
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With unknown physiological acceptors
Sysname
limonene:acceptor oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
(1) (S)-limonene + H2O + acceptor = (-)-perillyl alcohol + reduced acceptor [RN:R12591];
(2) (R)-limonene + H2O + acceptor = (+)-perillyl alcohol + reduced acceptor [RN:R12592]
Reaction(KEGG)
R12591 R12592
Substrate
(S)-limonene [CPD:C00521];
H2O [CPD:C00001];
acceptor [CPD:C00028];
(R)-limonene [CPD:C06099]
Product
(-)-perillyl alcohol [CPD:C02452];
reduced acceptor [CPD:C00030];
(+)-perillyl alcohol [CPD:C22272]
Comment
Contains FAD. The enzyme, characterized from the bacterium Castellaniella defragrans 65Phen, hydroxylates the R- and S-enantiomers at a similar rate. The in vivo electron acceptor may be a heterodimeric electron transfer flavoprotein (ETF).
History
EC 1.17.99.8 created 2020
Orthology
K24122  limonene dehydrogenase
Genes
CDN: BN940_14221 BN940_14226
Reference
1  [PMID:24952578]
  Authors
Petasch J, Disch EM, Markert S, Becher D, Schweder T, Huttel B, Reinhardt R, Harder J
  Title
The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen.
  Journal
BMC Microbiol 14:164 (2014)
DOI:10.1186/1471-2180-14-164
  Sequence
[cdn:BN940_14226 BN940_14221]
Reference
2  [PMID:29716998]
  Authors
Puentes-Cala E, Liebeke M, Markert S, Harder J
  Title
Limonene dehydrogenase hydroxylates the allylic methyl group of cyclic monoterpenes in the anaerobic terpene degradation by Castellaniella defragrans.
  Journal
J Biol Chem 293:9520-9529 (2018)
DOI:10.1074/jbc.RA117.001557
  Sequence
[cdn:BN940_14226 BN940_14221]
Other DBs
ExplorEnz - The Enzyme Database: 1.17.99.8
IUBMB Enzyme Nomenclature: 1.17.99.8
ExPASy - ENZYME nomenclature database: 1.17.99.8
BRENDA, the Enzyme Database: 1.17.99.8

  All links  
Chemical substance (7)   
   KEGG COMPOUND (7)   
Chemical reaction (3)   
   KEGG REACTION (2)   
   KEGG RCLASS (1)   
Gene (3)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (2)   
Protein sequence (4)   
   UniProt (2)   
   SWISS-PROT (2)   
Protein domain (1)   
   InterPro (1)   
All databases (18)   

Download RDF
DBGET integrated database retrieval system