Entry Name tetrachlorohydroquinone reductive dehalogenase;
Class Oxidoreductases;BRITE hierarchy
Sysname glutathione disulfide:2,6-dichlorohydroquinone (chlorinating)
Reaction(IUBMB) (1) 2,6-dichlorohydroquinone + Cl- + glutathione disulfide = 2,3,6-trichlorohydroquinone + 2 glutathione [RN:
R05403 ];
(2) 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide = 2,3,5,6-tetrachlorohydroquinone + 2 glutathione [RN:
R05402 ]
Reaction(KEGG) Substrate 2,6-dichlorohydroquinone [CPD:
C07097 ];
Cl- [CPD:
C00698 ];
glutathione disulfide [CPD:
C00127 ];
2,3,6-trichlorohydroquinone [CPD:
C07099 ]
Product 2,3,6-trichlorohydroquinone [CPD:
C07099 ];
glutathione [CPD:
C00051 ];
2,3,5,6-tetrachlorohydroquinone [CPD:
C03434 ]
Comment The enzyme, characterized from the bacterium Sphingobium chlorophenolicum, converts tetrachlorohydroquinone to 2,6-dichlorohydroquinone in two steps, via 2,3,6-trichlorohydroquinone, using glutathione as the reducing agent. The enzyme is sensitive to oxidation - when an internal L-cysteine residue is oxidized, the enzyme produces 2,3,5-trichloro-6-(glutathion-S-yl)-hydroquinone and 2,6-dichloro-3-(glutathion-S-yl)-hydroquinone instead of its normal products.
History EC 1.21.4.5 created 2018
Pathway ec00361 Chlorocyclohexane and chlorobenzene degradation
ec01120 Microbial metabolism in diverse environments
Orthology K15241 tetrachlorohydroquinone reductive dehalogenase
Genes Taxonomy Reference Authors Xun L, Topp E, Orser CS
Title Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp.
Journal Sequence Reference Authors McCarthy DL, Navarrete S, Willett WS, Babbitt PC, Copley SD
Title Exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione S-transferase superfamily.
Journal Other DBs ExplorEnz - The Enzyme Database: 1.21.4.5
ExPASy - ENZYME nomenclature database: 1.21.4.5
