KEGG ENZYME: 1.3.1.108

ENZYME: 1.3.1.108

Entry

EC 1.3.1.108                Enzyme

Name

caffeoyl-CoA reductase;
electron-bifurcating caffeoyl-CoA reductase;
caffeoyl-CoA reductase-Etf complex;
hydrocaffeoyl-CoA:NAD+,ferredoxin oxidoreductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

3-(3,4-dihydroxyphenyl)propanoyl-CoA:NAD+,ferredoxin oxidoreductase

Reaction(IUBMB)

3-(3,4-dihydroxyphenyl)propanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11674]

Reaction(KEGG)

R11674

Substrate

3-(3,4-dihydroxyphenyl)propanoyl-CoA [CPD:C21175];
NAD+ [CPD:C00003];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]

Product

(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl-CoA [CPD:C00323];
NADH [CPD:C00004];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Comment

The enzyme, characterized from the bacterium Acetobacterium woodii, contains two [4Fe-4S] clusters and FAD. The enzyme couples the endergonic ferredoxin reduction with NADH as reductant to the exergonic reduction of caffeoyl-CoA with the same reductant. It uses the mechanism of electron bifurcation to overcome the steep energy barrier in ferredoxin reduction. It also reduces 4-coumaroyl-CoA and feruloyl-CoA.

History

EC 1.3.1.108 created 2015

Orthology

K22430

caffeyl-CoA reductase-Etf complex subunit CarC

K22431

caffeyl-CoA reductase-Etf complex subunit CarD

K22432

caffeyl-CoA reductase-Etf complex subunit CarE

Genes

CFER:

D4Z93_06630 D4Z93_06635 D4Z93_06640

CCAA:

KQH81_14370 KQH81_14375 KQH81_14380

CLO:	HMPREF0868_0589 HMPREF0868_0590 HMPREF0868_0591(etfA)

CAPR:

EQM14_02005 EQM14_02010 EQM14_02015

CFEM:

HCR03_03805 HCR03_03810 HCR03_03815

AWO:	Awo_c15720(carC) Awo_c15730(carD) Awo_c15740(carE)

CSH:	Closa_3003 Closa_3004 Closa_3005

LGG:	QJR73_06855 QJR73_06860(etfB) QJR73_06865

CASP:

NQ535_01545

WHJ:	H9Q79_10320 H9Q79_10325 H9Q79_10330

LBW:	C3V36_10425 C3V36_10430 C3V36_10435

MDV:	C5Q96_01415

MNL:	QU661_02680

PIV:	NCTC13079_00081

» show all

Reference

1 [PMID:23479729]

Authors

Bertsch J, Parthasarathy A, Buckel W, Muller V

Title

An electron-bifurcating caffeyl-CoA reductase.

Journal

J Biol Chem 288:11304-11 (2013)
DOI:10.1074/jbc.M112.444919

Sequence

[awo:Awo_c15720 Awo_c15730 Awo_c15740]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.108

IUBMB Enzyme Nomenclature:

1.3.1.108

ExPASy - ENZYME nomenclature database:

1.3.1.108

BRENDA, the Enzyme Database:

1.3.1.108

DBGET integrated database retrieval system