KEGG ENZYME: 1.3.1.109

ENZYME: 1.3.1.109

Entry

EC 1.3.1.109                Enzyme

Name

butanoyl-CoA dehydrogenase complex (NAD+, ferredoxin);
bifurcating butyryl-CoA dehydrogenase;
butyryl-CoA dehydrogenase/Etf complex;
Etf-Bcd complex;
bifurcating butanoyl-CoA dehydrogenase;
butanoyl-CoA dehydrogenase/Etf complex;
butanoyl-CoA dehydrogenase (NAD+, ferredoxin)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

butanoyl-CoA:NAD+, ferredoxin oxidoreductase

Reaction(IUBMB)

butanoyl-CoA + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = (E)-but-2-enoyl-CoA + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R11675]

Reaction(KEGG)

R11675

Substrate

butanoyl-CoA [CPD:C00136];
NAD+ [CPD:C00003];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138]

Product

(E)-but-2-enoyl-CoA [CPD:C00877];
NADH [CPD:C00004];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]

Comment

The enzyme is a complex of a flavin-containing dehydrogenase component (Bcd) and an electron-transfer flavoprotein dimer (EtfAB). The enzyme complex, isolated from the bacteria Acidaminococcus fermentans and butanoate-producing Clostridia species, couples the exergonic reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA by NADH to the endergonic reduction of ferredoxin by NADH, using electron bifurcation to overcome the steep energy barrier in ferredoxin reduction.

History

EC 1.3.1.109 created 2015, modified 2021

Reference

1 [PMID:17993531]

Authors

Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer RK

Title

Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri.

Journal

J Bacteriol 190:843-50 (2008)
DOI:10.1128/JB.01417-07

Sequence

[ckl:CKL_0455 CKL_0456 CKL_0457]

Reference

2 [PMID:23772070]

Authors

Aboulnaga el-H, Pinkenburg O, Schiffels J, El-Refai A, Buckel W, Selmer T

Title

Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli.

Journal

J Bacteriol 195:3704-13 (2013)
DOI:10.1128/JB.00321-13

Sequence

[cdf:CD630_10540 CD630_10550 CD630_10560]

Reference

3 [PMID:24379410]

Authors

Chowdhury NP, Mowafy AM, Demmer JK, Upadhyay V, Koelzer S, Jayamani E, Kahnt J, Hornung M, Demmer U, Ermler U, Buckel W

Title

Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.

Journal

J Biol Chem 289:5145-57 (2014)
DOI:10.1074/jbc.M113.521013

Sequence

[afn:Acfer_1477 Acfer_0556 Acfer_0555]

Reference

4 [PMID:25903584]

Authors

Chowdhury NP, Kahnt J, Buckel W.

Title

Reduction of ferredoxin or oxygen by flavin-based electron bifurcation in Megasphaera elsdenii.

Journal

FEBS J 282:3149-60 (2015)
DOI:10.1111/febs.13308

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.109

IUBMB Enzyme Nomenclature:

1.3.1.109

ExPASy - ENZYME nomenclature database:

1.3.1.109

BRENDA, the Enzyme Database:

1.3.1.109

All links

Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
All databases (8)   

Download RDF

DBGET integrated database retrieval system