KEGG   ENZYME: 1.3.1.119
Entry
EC 1.3.1.119                Enzyme                                 
Name
chlorobenzene dihydrodiol dehydrogenase;
tecB (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol:NAD+ oxidoreductase
Reaction(IUBMB)
(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD+ = 3-chlorocatechol + NADH + H+ [RN:R06857]
Reaction(KEGG)
R06857;
(other) R05397 R06832
Substrate
(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol [CPD:C12837];
NAD+ [CPD:C00003]
Product
3-chlorocatechol [CPD:C05618];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This bacterial enzyme can transform various dihydrodiols of chlorobenzenes into the respective catechols, including the dihydrodiols of mono-, di-, tri-, and tetra-chlorinated benzenes. It also accepts the dihydrodiols of various chlorotoluenes. Substrates for the enzyme are generated by the broad spectrum EC 1.14.12.26, chlorobenzene dioxygenase.
History
EC 1.3.1.119 created 2018
Pathway
ec00361  Chlorocyclohexane and chlorobenzene degradation
ec01100  Metabolic pathways
Orthology
K16269  cis-1,2-dihydrobenzene-1,2-diol/chlorobenzene dihydrodiol dehydrogenase
Genes
PPF: Pput_2877
PPX: T1E_4270(tobD)
PMON: X969_10950
PMOT: X970_10605
PASI: LG197_17470(bphB)
BPT: Bpet3742(hcaB)
ATEM: PQV96_18690(bphB)
Reference
1  [PMID:8599538]
  Authors
Spiess E, Gorisch H
  Title
Purification and characterization of chlorobenzene cis-dihydrodiol dehydrogenase  from Xanthobacter flavus 14p1.
  Journal
Arch Microbiol 165:201-5 (1996)
DOI:10.1007/bf01692862
Reference
2  [PMID:11526005]
  Authors
Pollmann K, Beil S, Pieper DH.
  Title
Transformation of chlorinated benzenes and toluenes by Ralstonia sp. strain PS12  tecA (tetrachlorobenzene dioxygenase) and tecB (chlorobenzene dihydrodiol dehydrogenase) gene products.
  Journal
Appl Environ Microbiol 67:4057-63 (2001)
DOI:10.1128/AEM.67.9.4057-4063.2001
Reference
3  [PMID:15774876]
  Authors
Pollmann K, Wray V, Pieper DH
  Title
Chloromethylmuconolactones as critical metabolites in the degradation of chloromethylcatechols: recalcitrance of 2-chlorotoluene.
  Journal
J Bacteriol 187:2332-40 (2005)
DOI:10.1128/JB.187.7.2332-2340.2005
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.119
IUBMB Enzyme Nomenclature: 1.3.1.119
ExPASy - ENZYME nomenclature database: 1.3.1.119
BRENDA, the Enzyme Database: 1.3.1.119

  All links  
Pathway (5)   
   KEGG PATHWAY (4)   
   KEGG MODULE (1)   
Chemical substance (9)   
   KEGG COMPOUND (9)   
Chemical reaction (5)   
   KEGG REACTION (3)   
   KEGG RCLASS (2)   
Gene (9)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (8)   
Protein sequence (2)   
   UniProt (1)   
   SWISS-PROT (1)   
Protein domain (5)   
   InterPro (5)   
All databases (35)   

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