KEGG ENZYME: 1.3.1.81

ENZYME: 1.3.1.81

Entry

EC 1.3.1.81                 Enzyme

Name

(+)-pulegone reductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

(-)-menthone:NADP+ oxidoreductase

Reaction(IUBMB)

(1) (-)-menthone + NADP+ = (+)-pulegone + NADPH + H+ [RN:R06419];
(2) (+)-isomenthone + NADP+ = (+)-pulegone + NADPH + H+ [RN:R06420]

Reaction(KEGG)

R06419 R06420

Substrate

(-)-menthone [CPD:C00843];
NADP+ [CPD:C00006];
(+)-isomenthone [CPD:C11952]

Product

(+)-pulegone [CPD:C09893];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

NADH cannot replace NADPH as reductant. The Delta8,9-double bond of (+)-cis-isopulegone and the Delta1,2-double bond of (+/-)-piperitone are not substrates. The enzyme from peppermint (Mentha x piperita) converts (+)-pulegone into both (-)-menthone and (+)-isomenthone at a ratio of 70:30 for native enzyme but it does not catalyse the reverse reaction. This enzyme is a member of the medium-chain dehydrogenase/reductase superfamily.

History

EC 1.3.1.81 created 2008

Pathway

ec00902

Monoterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K15092

(+)-pulegone reductase

Reference

1 [PMID:13679086]

Authors

Ringer KL, McConkey ME, Davis EM, Rushing GW, Croteau R

Title

Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint.

Journal

Arch Biochem Biophys 418:80-92 (2003)
DOI:10.1016/S0003-9861(03)00390-4

Sequence

[ag:AAQ75423]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.1.81

IUBMB Enzyme Nomenclature:

1.3.1.81

ExPASy - ENZYME nomenclature database:

1.3.1.81

BRENDA, the Enzyme Database:

1.3.1.81

DBGET integrated database retrieval system