KEGG ENZYME: 1.3.3.16

ENZYME: 1.3.3.16

Entry

EC 1.3.3.16                 Enzyme

Name

oxazoline dehydrogenase;
azoline oxidase;
thiazoline oxidase;
cyanobactin oxidase;
patG (gene name);
mcaG (gene name);
artG (gene name);
lynG (gene name);
tenG (gene name)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With oxygen as acceptor

Sysname

a [protein]-2-oxazoline:oxygen oxidoreductase (2-oxazole-forming)

Reaction(IUBMB)

(1) a [protein]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline + O2 = a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-thiazole + H2O2;
(2) a [protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-2-oxazoline + O2 = a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-oxazole + H2O2;
(3) a [protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-5-methyl-2-oxazoline + O2 = a [protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-5-methyl-1,3-oxazole + H2O2

Substrate

[protein]-(1S,4R)-2-(C-substituted-aminomethyl)-4-acyl-2-thiazoline;
O2 [CPD:C00007];
[protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-2-oxazoline;
[protein]-(S,S)-2-(C-substituted-aminomethyl)-4-acyl-5-methyl-2-oxazoline

Product

[protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-thiazole;
H2O2 [CPD:C00027];
[protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-1,3-oxazole;
[protein]-(S)-2-(C-substituted-aminomethyl)-4-acyl-5-methyl-1,3-oxazole

Comment

Contains FMN. This enzyme oxidizes 2-oxazoline, 5-methyl-2-oxazoline, and 2-thiazoline within peptides, which were formed by EC 6.2.2.2, oxazoline synthase, and EC 6.2.2.3, thiazoline synthase, to the respective pyrrole-type rings. The enzyme is found as either a stand-alone protein or as a domain within a multifunctional protein (the G protein) that also functions as a peptidase.

History

EC 1.3.3.16 created 2020

Orthology

K05897

oxazoline/thiazoline dehydrogenase

K24454

thiazoline dehydrogenase / protease

Genes

SVR:	CP971_15255

NFS:	OIE67_41180

MHAI:

OHB01_03390

MCHL:

PVK74_24145

MPRN:

Q3V37_25160

ASIC:

Q0Z83_071180

ATL:	Athai_48170

DVC:	Dvina_40860

CYN:	Cyan7425_0520

KOV:	K9N68_13670 K9N68_24400

LEP:	Lepto7376_0890

CYJ:	Cyan7822_6148 Cyan7822_6534

TER:

Tery_2359

ARP:	NIES39_O00960

ONI:	Osc7112_3655

MPRO:

BJP34_18465

MIC:	Mic7113_0885

RIV:	Riv7116_6338

NCN:	BZZ01_09390

PLP:	Ple7327_1613

KBB:	ccbrp13_09210 ccbrp13_65820

LCRE:

Pla8534_19290

FTJ:

FTUN_1197

» show all

Reference

1 [PMID:8895467]

Authors

Li YM, Milne JC, Madison LL, Kolter R, Walsh CT

Title

From peptide precursors to oxazole and thiazole-containing peptide antibiotics: microcin B17 synthase.

Journal

Science 274:1188-93 (1996)
DOI:10.1126/science.274.5290.1188

Reference

2 [PMID:15883371]

Authors

Schmidt EW, Nelson JT, Rasko DA, Sudek S, Eisen JA, Haygood MG, Ravel J

Title

Patellamide A and C biosynthesis by a microcin-like pathway in Prochloron didemni, the cyanobacterial symbiont of Lissoclinum patella.

Journal

Proc Natl Acad Sci U S A 102:7315-20 (2005)
DOI:10.1073/pnas.0501424102

Sequence

[ag:AAY21156]

Reference

3 [PMID:27841750]

Authors

Bent AF, Mann G, Houssen WE, Mykhaylyk V, Duman R, Thomas L, Jaspars M, Wagner A, Naismith JH

Title

Structure of the cyanobactin oxidase ThcOx from Cyanothece sp. PCC 7425, the first structure to be solved at Diamond Light Source beamline I23 by means of S-SAD.

Journal

Acta Crystallogr D Struct Biol 72:1174-1180 (2016)
DOI:10.1107/S2059798316015850

Sequence

[cyn:Cyan7425_0520]

Reference

4 [PMID:30661981]

Authors

Ghilarov D, Stevenson CEM, Travin DY, Piskunova J, Serebryakova M, Maxwell A, Lawson DM, Severinov K

Title

Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.

Journal

Mol Cell 73:749-762.e5 (2019)
DOI:10.1016/j.molcel.2018.11.032

Other DBs

ExplorEnz - The Enzyme Database:

1.3.3.16

IUBMB Enzyme Nomenclature:

1.3.3.16

ExPASy - ENZYME nomenclature database:

1.3.3.16

BRENDA, the Enzyme Database:

1.3.3.16

All links

Chemical substance (2)   
   KEGG COMPOUND (2)   
Gene (37)   
   KEGG ORTHOLOGY (2)   
   KEGG GENES (27)   
   KEGG MGENES (8)   
All databases (39)   

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