KEGG ENZYME: 1.3.4.1

ENZYME: 1.3.4.1

Entry

EC 1.3.4.1                  Enzyme

Name

fumarate reductase (CoM/CoB);
thiol:fumarate reductase;
Tfr

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With a disulfide as acceptor

Sysname

fumarate CoM:CoB oxidoreductase (succinate-forming)

Reaction(IUBMB)

fumarate + CoM + CoB = succinate + CoM-S-S-CoB [RN:R10660]

Reaction(KEGG)

R10660

Substrate

fumarate [CPD:C00122];
CoM [CPD:C03576];
CoB [CPD:C04628]

Product

succinate [CPD:C00042];
CoM-S-S-CoB [CPD:C04832]

Comment

The enzyme, isolated from the archaeon Methanobacterium thermoautotrophicum, is very oxygen sensitive. It cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor. Distinct from EC 1.3.1.6 [fumarate reductase (NADH)], EC 1.3.5.1 [succinate dehydrogenase (ubiquinone)], and EC 1.3.5.4 [fumarate reductase (quinol)].

History

EC 1.3.4.1 created 2014 as EC 1.3.98.2, transferred 2014 to EC 1.3.4.1

Pathway

ec00720

Carbon fixation pathways in prokaryotes

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18209

fumarate reductase (CoM/CoB) subunit A

K18210

fumarate reductase (CoM/CoB) subunit B

Genes

LCJ:	NCTC11976_02789(nadB_2)

EPS:	L0Y14_11940

BGE:	BC1002_5768

BFN:

OI25_7370

PSPW:

BJG93_06520

PKF:	RW095_05110 RW095_24620

PPUL:

RO07_01465

PSPU:

NA29_12930

PNR:	AT302_16660

NPV:	OHM77_12775

SLAC:

SKTS_06150

XDI:	EZH22_13185

HMC:	HYPMC_0241

RHZ:	RHPLAN_26730

RSQ:	Rsph17025_1547

CAZT:

LV780_06990

PFEO:

E3U26_14950

SPHI:

TS85_13175

SRF:	LHU95_15895

RAP:

RHOA_4370

CSPF:

CSF_1076

CCOR:

CCORG_0898

GEF:	FO488_11755

DMS:	E8L03_00165

BACO:

OXB_3153

FEC:	QNH15_23770

RUE:	DT065_10055

NCM:	QNK12_18805

ALKG:

MOJ78_01935

DOM:	RRU94_18845

PNP:	IJ22_25670 IJ22_47240

POW:	IJ21_10130

PYG:	AWM70_00700

PKP:	SK3146_05406(frdA_1)

AACO:

K1I37_00670

EFF:	skT53_06110 skT53_13780

ACOL:

K5I23_15405

PETR:

QKW49_19270

DSY:

DSY3532

DHD:

Dhaf_1873

DMT:	DESME_00810

DKU:	Desku_0815

FWA:	DCMF_09465 DCMF_09620 DCMF_14880

CCOM:

I6K69_15150

BLIQ:

INP51_11880

LACY:

A4V08_05955

CSCI:

HDCHBGLK_01871 HDCHBGLK_01882

CSH:	Closa_1173

CBOL:

CGC65_07300 CGC65_22960

MDV:	C5Q96_06350

PBQ:	C3V37_06420

TPZ:	Tph_c24010

GEK:	kuro4_13740 kuro4_28190

TEP:	TepRe1_0409

TAE:	TepiRe1_0453

BACG:

D2962_02060 D2962_02695

HALS:

D7D81_18240

ATE:

Athe_2084

COB:	COB47_1860

COW:	Calow_1774

CKN:	Calkro_0547

CCHA:

ELD05_03760

CDIA:

CaldiYA01_05050

CDAN:

SOJ16_002198

FMA:

FMG_0304

PIV:	NCTC13079_01033

STED:

SPTER_31650(nadB)

FCZ:	IMF26_04890

ELE:

Elen_0252

AHAT:

ADCFC_08680 ADCFC_08710

NIE:	KV110_17120

NCA:

Noca_0032

PSUU:

Psuf_024840

KBS:	EPA93_42720

ELUT:

CKA38_10925

BHC:	JFL75_16050

BHD:	BHYOB78_11155

BRM:	Bmur_0641 Bmur_2561

BPO:	BP951000_2187

BPJ:	B2904_orf1096 B2904_orf718

BPIP:

BPP43_04180

BPW:	WESB_1607 WESB_1949

BIP:

Bint_2001

FUL:	C4N20_09145

CPOR:

BED41_11845

MJA:	MJ_0033 MJ_0092

MFE:	Mefer_0390 Mefer_1238

MVU:	Metvu_1248 Metvu_1286

MFS:	MFS40622_0414 MFS40622_0946

MIF:	Metin_0533 Metin_0972

MJH:	JH146_0495 JH146_1538

MESG:

MLAUSG7_0989 MLAUSG7_1167

MESA:

MLASG1_0610 MLASG1_0788

MIG:	Metig_0017 Metig_0562

MMP:	MMP1067 MMP1277(frdA)

MMQ:	MmarC5_0315 MmarC5_0522

MMX:	MmarC6_1397 MmarC6_1592

MMZ:	MmarC7_0320 MmarC7_0522

MMD:	GYY_06170 GYY_07220(sdhA)

MMAK:

MMKA1_12460(tfrB) MMKA1_15020(tfrA)

MMAO:

MMOS7_11760(tfrB) MMOS7_13840(tfrA)

MMAD:

MMJJ_15680(frdA) MMJJ_17830(frdB)

MAE:	Maeo_0245 Maeo_0717

MVN:	Mevan_0390 Mevan_0588

MVO:	Mvol_0038 Mvol_0098 Mvol_0369

MOK:	Metok_0260 Metok_1350

METF:

CFE53_06655 CFE53_06830

MTH:	MTH_1502 MTH_1850

MMG:	MTBMA_c00880(tfrA) MTBMA_c04210(tfrB)

MWO:	MWSIV6_0043(tfrA) MWSIV6_0381(tfrB)

MTEE:

MTTB_00770 MTTB_12870

METC:

MTCT_1366 MTCT_1689

METE:

tca_01449(nadB) tca_01787(frdB)

METZ:

METMT2_0107 METMT2_1345

METK:

FVF72_02455 FVF72_04100

MTHM:

FZP57_00160 FZP57_01780

METJ:

FZP68_01540 FZP68_06805

MST:	Msp_0180(tfrA) Msp_1044(tfrB)

METB:

AW729_06205(sdhA) AW729_10665

MEIS:

PXD04_02390(tfrB) PXD04_04780(tfrA)

MRU:	mru_0088(sdhA) mru_0655(sdhB) mru_1212(hdrB2)

MSI:	Msm_0393 Msm_1258

MEB:	Abm4_0015(sdhA) Abm4_1340(sdhB)

MMIL:

sm9_0007(sdhA) sm9_0612(sdhB)

MEYE:

TL18_00105(sdhA) TL18_02920

MOL:	YLM1_0294 YLM1_1577

MARO:

MarbSA_07560 MarbSA_11160

METV:

K4897_02485 K4897_05045

MEL:	Metbo_0043 Metbo_0664

MEW:	MSWAN_0058 MSWAN_1693

METH:

MBMB1_0037 MBMB1_0259 MBMB1_1319

MFC:	BRM9_0092(sdhA) BRM9_0542(sdhB)

MFI:	DSM1535_0523 DSM1535_1410

MCUB:

MCBB_0053(tfrA) MCBB_1554

MSUB:

BK009_05580 BK009_09905

METN:

BK008_01125 BK008_06905

METT:

CIT01_03165 CIT01_05860 CIT01_08295

METO:

CIT02_06880 CIT02_11385

MEME:

HYG87_06795 HYG87_08750

MFEG:

GCM10025860_08050 GCM10025860_26970

MFV:	Mfer_0085 Mfer_0228

MKA:	MK0132(frdB_glpC) MK0498 MK0828(sdhA)

FPL:

Ferp_1387

TGA:

TGAM_1022

TSI:

TSIB_0772

THE:

GQS_01385

TGY:	X802_09190

TRL:	A3L10_02300

PPAC:

PAP_08545

MBY:	MSBRM_0047

MBAR:

MSBR2_0046

MBAK:

MSBR3_0027

MMA:

MM_1290

MMAZ:

MmTuc01_1336

MMJ:	MSMAS_0025

MMAC:

MSMAC_0025

MVC:	MSVAZ_0061

MEK:	MSKOL_0057

MLS:	MSLAZ_0024

METM:

MSMTP_0027

MEF:	MSWH1_0026

MEQ:	MSWHS_0027

MTHR:

MSTHT_1799

MTHE:

MSTHC_1487

MHOR:

MSHOH_0025

MMET:

MCMEM_0031 MCMEM_1454

MELO:

J7W08_10190

MMH:

Mmah_1949

MHAZ:

BHR79_03890

MEV:	Metev_0037

MZH:	Mzhil_0031

MPY:

Mpsy_1801

MZI:	HWN40_01325

MHZ:	Metho_2408

MTP:

Mthe_1652

MCJ:	MCON_3042(hdrD)

MHI:

Mhar_0709

MHU:	Mhun_3052 Mhun_3053

MRTJ:

KHC33_01635 KHC33_01640

MLA:	Mlab_0139 Mlab_0140

MEM:	Memar_0220 Memar_0221

MBG:	BN140_0102(sdhB) BN140_0103(sdhA)

MEMA:

MMAB1_0142 MMAB1_0144

MCHK:

MchiMG62_03460 MchiMG62_03470

MSUM:

OH143_07845 OH143_07850

MRC:	R6Y96_01290(tfrA) R6Y96_01295(tfrB)

MPI:	Mpet_2648 Mpet_2649

MEND:

L6E24_08655 L6E24_08660

MEFW:

F1737_00215 F1737_00220

MAQE:

RJ40_08785 RJ40_08790

MFK:	E2N92_08985 E2N92_08990

MOU:	OU421_05760 OU421_05765

MESB:

L1S32_09100 L1S32_09105

MANQ:

L1994_00360 L1994_00365

MBN:	Mboo_0085 Mboo_0086

MFO:	Metfor_0059 Metfor_0060

MPL:	Mpal_0402 Mpal_0403

MPD:	MCP_0282(hdrD-1) MCP_1107(hdrD-2)

MEZ:	Mtc_0078 Mtc_1543 Mtc_2258

RCI:	LRC216 LRC333 RCIX1755 RCIX780

TAR:	TALC_01391

MAX:	MMALV_14890

MER:	MMINT_04140

MARC:

AR505_0168

BARC:

AOA65_0073 AOA65_0074 AOA65_2161(nadB_2)

LOB:	NEF87_003194

» show all

Reference

1 [PMID:2499256]

Authors

Khandekar SS, Eirich LD

Title

Purification and characterization of an anabolic fumarate reductase from Methanobacterium thermoautotrophicum.

Journal

Appl Environ Microbiol 55:856-61 (1989)
DOI:10.1128/AEM.55.4.856-861.1989

Reference

2 [PMID:9578488]

Authors

Heim S, Kunkel A, Thauer RK, Hedderich R

Title

Thiol:fumarate reductase (Tfr) from Methanobacterium thermoautotrophicum--identification of the catalytic sites for fumarate reduction and thiol oxidation.

Journal

Eur J Biochem 253:292-9 (1998)
DOI:10.1046/j.1432-1327.1998.2530292.x

Sequence

[mmg:MTBMA_c00880 MTBMA_c04210]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.4.1

IUBMB Enzyme Nomenclature:

1.3.4.1

ExPASy - ENZYME nomenclature database:

1.3.4.1

BRENDA, the Enzyme Database:

1.3.4.1

DBGET integrated database retrieval system