KEGG ENZYME: 1.3.8.13

ENZYME: 1.3.8.13

Entry

EC 1.3.8.13                 Enzyme

Name

crotonobetainyl-CoA reductase;
caiA (gene name)

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With a flavin as acceptor

Sysname

gamma-butyrobetainyl-CoA:electron-transfer flavoprotein 2,3-oxidoreductase

Reaction(IUBMB)

gamma-butyrobetainyl-CoA + electron-transfer flavoprotein = crotonobetainyl-CoA + reduced electron-transfer flavoprotein [RN:R11600]

Reaction(KEGG)

R11600

Substrate

gamma-butyrobetainyl-CoA [CPD:C20749];
electron-transfer flavoprotein [CPD:C04253]

Product

crotonobetainyl-CoA [CPD:C20748];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

The enzyme has been purified from the bacterium Escherichia coli O44 K74, in which it forms a complex with EC 2.8.3.21, L-carnitine CoA-transferase. The electron donor is believed to be an electron-transfer flavoprotein (ETF) encoded by the fixA and fixB genes.

History

EC 1.3.8.13 created 2017

Orthology

K08297

crotonobetainyl-CoA dehydrogenase

Genes

ECO:	b0039(caiA)

ECJ:	JW0038(caiA)

ECD:	ECDH10B_0040(caiA)

EBW:	BWG_0037(caiA)

ECOK:

ECMDS42_0032(caiA)

ECOC:

C3026_00205

ECE:	Z0045(caiA)

ECS:	ECs_0042(caiA)

ECF:	ECH74115_0043(caiA)

ETW:	ECSP_0042(caiA)

ELX:	CDCO157_0041

EOI:	ECO111_0040(caiA)

EOJ:	ECO26_0040(caiA)

EOH:	ECO103_0041(caiA)

ECOO:

ECRM13514_0040(caiA)

ECOH:

ECRM13516_0042(caiA)

ESL:

O3K_21350

ESO:

O3O_04035

ESM:

O3M_21250

ECK:	EC55989_0039(caiA)

ECG:	E2348C_0040(caiA)

EOK:	G2583_0041(caiA)

ELR:	ECO55CA74_00195

ELH:

ETEC_0039

ECW:	EcE24377A_0041(caiA)

EUN:

UMNK88_39

ECP:

ECP_0039

ENA:	ECNA114_0026(caiA)

ECOS:

EC958_0173(caiA)

ECV:	APECO1_1942(caiA)

ECOA:

APECO78_03655

ECX:	EcHS_A0043(caiA)

ECM:	EcSMS35_0040(caiA)

ECY:

ECSE_0040

ECR:	ECIAI1_0041(caiA)

ECQ:	ECED1_0038(caiA)

EUM:	ECUMN_0041(caiA)

ECT:	ECIAI39_0040(caiA)

EOC:	CE10_0040(caiA)

EBR:	ECB_00043(caiA)

ECOB:

C3029_03950

EBL:	ECD_00043(caiA)

EBE:	B21_00042(caiA)

EBD:

ECBD_3576

ECI:	UTI89_C0045(caiA)

EIH:	ECOK1_0038(caiA)

ECZ:	ECS88_0042(caiA)

ECC:	c0048(caiA)

ELO:	EC042_0041(caiA)

ELN:	NRG857_00205

ESE:

ECSF_0044

ECL:	EcolC_3616

EKO:	EKO11_3873

EKF:	KO11_00195(caiA)

EAB:	ECABU_c00440(caiA)

EDH:	EcDH1_3560

EDJ:	ECDH1ME8569_0038(caiA)

ELU:	UM146_22975

ELW:	ECW_m0039(caiA)

ELL:	WFL_00195(caiA)

ELC:	i14_0041(caiA)

ELD:	i02_0041(caiA)

ELP:	P12B_c0034(caiA)

ELF:	LF82_0257(caiA)

ECOL:

LY180_00200

ECOI:

ECOPMV1_00040(caiA_1)

ECOJ:

P423_00195

EFE:	EFER_0047(caiA)

EAL:	EAKF1_ch1383(caiA)

EMA:	C1192_12775

ESZ:	FEM44_14690(caiA)

ERUY:

OSH18_09410(caiA)

STY:	STY0083(caiA)

STT:	t0074(caiA)

SEX:	STBHUCCB_840

SENT:

TY21A_00380

STM:	STM0073(caiA)

SEO:	STM14_0087(caiA)

SEV:	STMMW_00751

SEY:	SL1344_0074(caiA)

SEM:	STMDT12_C00740

SEJ:	STMUK_0074(caiA)

SEB:	STM474_0076(caiA)

SEF:	UMN798_0081(caiA)

SETU:

STU288_00365

SETC:

CFSAN001921_17055

SENR:

STMDT2_00741(caiA)

SEND:

DT104_00741(caiA)

SENI:

CY43_00360

SEEN:

SE451236_06375

SPT:	SPA0074(caiA)

SEK:

SSPA0070

SPQ:	SPAB_00090

SEI:	SPC_0078(caiA)

SEC:	SCH_0067(caiA)

SEH:	SeHA_C0077(caiA)

SHB:

SU5_0708

SENH:

CFSAN002069_08865

SEEH:

SEEH1578_09395

SEE:	SNSL254_A0077(caiA)

SENN:

SN31241_10520

SEW:	SeSA_A0081(caiA)

SEA:	SeAg_B0080(caiA)

SENS:

Q786_00360

SED:	SeD_A0079(caiA)

SEG:	SG0076(caiA)

SEL:	SPUL_0078(caiA)

SEGA:

SPUCDC_0078(caiA)

SET:	SEN0074(caiA)

SENA:

AU38_00360

SENO:

AU37_00360

SENV:

AU39_00360

SENQ:

AU40_00395

SENL:

IY59_00375

SENJ:

CFSAN001992_10660

SEEC:

CFSAN002050_06815

SEEB:

SEEB0189_019020

SEEP:

I137_00340

SENB:

BN855_760(caiA)

SENE:

IA1_00370

SENC:

SEET0819_07255

SES:	SARI_02927

SBG:	SBG_0058(caiA)

SBZ:

A464_61

SBV:	N643_00280

SALZ:

EOS98_18875

SFL:	SF0036(caiA)

SFX:	S0038(caiA)

SFV:	SFV_0033(caiA)

SFE:	SFxv_0037(caiA)

SFN:

SFy_0045

SFS:

SFyv_0049

SFT:	NCTC1_00036(caiA)

SSN:	SSON_0047(caiA)

SDY:	SDY_0061(caiA)

SDZ:	Asd1617_00066

SHQ:	A0259_04035

CRO:	ROD_00411(caiA)

CKO:

CKO_03344

CFD:	CFNIH1_09905

CBRA:

A6J81_13205

CWE:	CO701_18215

CYO:	CD187_20355

CPOT:

FOB25_07450(caiA)

CFQ:	C2U38_03525

CSED:

JY391_18140(caiA)

CAMA:

F384_00210

CAF:	AL524_22255

CIF:	AL515_05000

CFAR:

CI104_04650

CIR:	C2U53_04680

CIE:	AN232_26285

CPAR:

CUC49_00190

CTEL:

GBC03_25645(caiA)

CITZ:

E4Z61_13390(caiA)

CARS:

E1B03_04740(caiA)

CIX:	M4I31_19965(caiA)

CIB:	HF677_019720(caiA)

CENS:

P2W74_19330(caiA)

EBT:	EBL_c33140(caiA)

ESC:	Entcl_2475

KLE:	AO703_09860

ASUB:

NLZ15_03200(caiA)

YRE:	HEC60_04865(caiA)

KIN:	AB182_21535

PDZ:	HHA33_24100(caiA)

METY:

MRY16398_47840(caiA)

PSGC:

G163CM_28640(caiA)

TOE:	QMG90_18000(caiA)

EBF:

D782_3830

EBC:	C2U52_03230

EBB:

F652_2816

PMR:	PMI2655(caiA)

PMIB:

BB2000_2646(caiA)

PVL:	AOB99_14510

PVG:	CRN77_06415

PHAU:

PH4a_04750

PROT:

BTA34_14490

PCOL:

F1325_16705(caiA)

PCIB:

F9282_17050(caiA)

PPEE:

I6G31_11900(caiA)

PRG:	RB151_007300(caiA_2)

PHEI:

NCTC12003_00738(caiA)

PRQ:	CYG50_11995(caiA)

PHAG:

PZ638_03510(caiA)

EIC:	NT01EI_2959

ETR:	ETAE_2663(caiA)

ETD:

ETAF_2401

ETE:

ETEE_0764

ETC:	ETAC_12795

EDW:	QY76_07180

EDL:	AAZ33_14085(caiA)

EHO:	A9798_03955

HAV:	AT03_19300

HPAR:

AL518_07295

OPO:	DSM2777_06085

LPV:	HYN51_05075

PRAG:

EKN56_12490(caiA)

LRI:	NCTC12151_02504(caiA)

SLO:

Shew_2675

SSE:

Ssed_3228

SPL:

Spea_4037

SHL:

Shal_0221

SWP:

swp_4937

SALG:

BS332_05315 BS332_05360

SLJ:	EGC82_18075

SCAA:

TUM17387_35670(caiA_1) TUM17387_35760(caiA_2)

SCHK:

GII14_20175(caiA) GII14_20235(caiA)

SAEG:

K0H80_13660(caiA)

SSPA:

K0I31_13905(caiA)

SHNS:

K0J45_13750(caiA)

SRHS:

K0I63_13835(caiA)

CPIN:

CPIN18020_0491(caiA)

CRX:	CRECT_1851(caiA)

AHS:	AHALO_2624(caiA)

AMAR:

AMRN_2723(caiA)

PBIZ:

LWC08_01450(caiA)

BACI:

B1NLA3E_21285

CAPR:

EQM14_13825

DRM:

Dred_0570

RHO:	RHOM_09505

CSH:	Closa_3931

CALK:

HUE98_15945

ELE:

Elen_1834

EYY:	EGYY_17990(CaiA)

EGD:	GS424_010370(caiA)

CBAC:

JI75_07140

BWA:	HLV38_02055(caiA)

XYL:	ET495_16520(caiA)

CELC:

K5O09_10670(caiA)

OEK:	FFI11_010750(caiA)

GYU:	FE374_07885(caiA)

PFR:	PFREUD_02420(caiA)

PFRE:

RM25_0219

AHE:

Arch_1363

ARCA:

HC352_07040(caiA)

TPY:	CQ11_04205

TPYO:

X956_03580

TPEC:

HLG82_02695(caiA)

TBER:

QPC17_02455(caiA)

AMY:	ADJ76_00875

FSL:	EJO69_02440

WNE:	PIG85_00160(caiA)

VCB:	CYK25_001665(caiA)

PHON:

BH719_04000

» show all

Reference

1 [PMID:8060125]

Authors

Roth S, Jung K, Jung H, Hommel RK, Kleber HP

Title

Crotonobetaine reductase from Escherichia coli--a new inducible enzyme of anaerobic metabolization of L(-)-carnitine.

Journal

Antonie Van Leeuwenhoek 65:63-9 (1994)
DOI:10.1007/BF00878280

Reference

2 [PMID:10209289]

Authors

Preusser A, Wagner U, Elssner T, Kleber HP

Title

Crotonobetaine reductase from Escherichia coli consists of two proteins.

Journal

Biochim Biophys Acta 1431:166-78 (1999)
DOI:10.1016/S0167-4838(99)00032-1

Sequence

[eco:b0039]

Reference

3 [PMID:10978161]

Authors

Elssner T, Hennig L, Frauendorf H, Haferburg D, Kleber HP

Title

Isolation, identification, and synthesis of gamma-butyrobetainyl-CoA and crotonobetainyl-CoA, compounds involved in carnitine metabolism of E. coli.

Journal

Biochemistry 39:10761-9 (2000)
DOI:10.1021/bi000776c

Reference

4 [PMID:12081978]

Authors

Walt A, Kahn ML

Title

The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli.

Journal

J Bacteriol 184:4044-7 (2002)
DOI:10.1128/JB.184.14.4044-4047.2002

Other DBs

ExplorEnz - The Enzyme Database:

1.3.8.13

IUBMB Enzyme Nomenclature:

1.3.8.13

ExPASy - ENZYME nomenclature database:

1.3.8.13

BRENDA, the Enzyme Database:

1.3.8.13

DBGET integrated database retrieval system