KEGG ENZYME: 1.3.99.30

ENZYME: 1.3.99.30

Entry

EC 1.3.99.30                Enzyme

Name

phytoene desaturase (3,4-didehydrolycopene-forming);
5-step phytoene desaturase;
five-step phytoene desaturase;
phytoene desaturase (ambiguous);
Al-1

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors

Sysname

15-cis-phytoene:acceptor oxidoreductase (3,4-didehydrolycopene-forming)

Reaction(IUBMB)

15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor (overall reaction) [RN:R09703];
(1a) 15-cis-phytoene + acceptor = all-trans-phytofluene + reduced acceptor [RN:R09692];
(1b) all-trans-phytofluene + acceptor = all-trans-zeta-carotene + reduced acceptor [RN:R04787];
(1c) all-trans-zeta-carotene + acceptor = all-trans-neurosporene + reduced acceptor [RN:R04798];
(1d) all-trans-neurosporene + acceptor = all-trans-lycopene + reduced acceptor [RN:R04800];
(1e) all-trans-lycopene + acceptor = all-trans-3,4-didehydrolycopene + reduced acceptor [RN:R07518]

Reaction(KEGG)

R04787 R04798 R04800 R07518 R09692 R09703

Substrate

15-cis-phytoene [CPD:C05421];
acceptor [CPD:C00028];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]

Product

all-trans-3,4-didehydrolycopene [CPD:C15867];
reduced acceptor [CPD:C00030];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]

Comment

This enzyme is involved in carotenoid biosynthesis and catalyses up to five desaturation steps (cf. EC 1.3.99.28 [phytoene desaturase (neurosporene-forming)], EC 1.3.99.29 [phytoene desaturase (zeta-carotene-forming)] and EC 1.3.99.31 [phytoene desaturase (lycopene-forming)]).

History

EC 1.3.99.30 created 2011

Pathway

ec00906

Carotenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K15745

phytoene desaturase (3,4-didehydrolycopene-forming)

Genes

CNS:	116339530 116349508

BCOO:

119078125

AGIF:

122854590 122854592 122854593

API:	100159050 100161380 100169110 100169245(tor)

DNX:

107165446

AGS:

114125979

RMD:	113548062 113549968

ACOO:

126834667 126834668

DVT:

126905570

VJA:

111273439

TUT:	107364307 107364309 107365911

VCN:	VOLCADRAFT_96696

MNG:

MNEG_6064

CVR:	CHLNCDRAFT_48542

OLU:	OSTLU_29222

OTA:	OT_ostta01g05990

BPG:	Bathy15g02080

MIS:	MICPUN_85926

MPP:	MICPUCDRAFT_36095(JGI:MicpuC2_36095)

NCR:	NCU00552(al-1)

NTE:	NEUTE1DRAFT92886(NEUTE1DRAFT_92886)

SMP:	SMAC_01244(putative_al1)

PAN:	PODANSg3970

PBEL:

QC761_115010(AL1)

PPSD:

QC762_115010(AL1)

PPSP:

QC763_115010(AL1)

PPSA:

QC764_115010(AL1)

FGR:	FGSG_03065

FPU:	FPSE_02161

FPOA:

FPOAC1_005422

FVN:	FVRRES_05979

FVR:	FVEG_10717

FOX:	FOXG_12143

NHE:	NECHADRAFT_97347

FFC:	NCS54_01343900

FKR:	NCS57_01320600

FMU:	J7337_013306

VAL:	VDBG_00708

VDA:	VDAG_00320

CFJ:	CFIO01_11136

CLUP:

CLUP02_08750

CHIG:

CH63R_07461

ELA:	UCREL1_7363

PFY:	PFICI_09859

SSL:	SS1G_04340

BFU:	BCIN_01g04550(Bcphd1)

MBE:

MBM_00660

PSCO:

LY89DRAFT_686853

GLZ:	GLAREA_08046

AOR:	AO090020000158

AFV:	AFLA_010500

ALUC:

AKAW2_12022A AKAW2_61045S AKAW2_61046A

POU:	POX_a01893 POX_a01895

ABE:

ARB_03950

TVE:

TRV_03238

PNO:	SNOG_00342

PTE:

PTT_09941

BZE:	COCCADRAFT_96840

BSC:	COCSADRAFT_304602

BOR:	COCMIDRAFT_88940

AALT:

CC77DRAFT_951686

ARAB:

EKO05_0004494

ZTR:	MYCGRDRAFT_101921

PFJ:	MYCFIDRAFT_29348

FFU:	CLAFUR5_14250

CBET:

CB0940_02240

BCOM:

BAUCODRAFT_120791

NPA:	UCRNP2_7258

TMS:	TREMEDRAFT_34017

SHS:	STEHIDRAFT_99350

UMA:	UMAG_04210

PFP:	PFL1_06723

SGRA:

EX895_001661

PGR:	PGTG_19044

PTRC:

PtA15_8A421

PSTR:

Pst134EA_015845

MLR:	MELLADRAFT_40795

ACAN:

ACA1_391690

SMIN:

v1.2.005646.t1(symbB.v1.2.005646.t1)

PTI:	PHATR_43904

FCY:	FRACYDRAFT_195646(PDH1) FRACYDRAFT_231974

EHX:	EMIHUDRAFT_458688

PBF:	CFX0092_B0152

» show all

Reference

1 [PMID:11017770]

Authors

Hausmann A, Sandmann G

Title

A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa.

Journal

Fungal Genet Biol 30:147-53 (2000)
DOI:10.1006/fgbi.2000.1212

Sequence

[ncr:NCU00552]

Reference

2 [PMID:18812228]

Authors

Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S

Title

Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation.

Journal

Fungal Genet Biol 45:1497-505 (2008)
DOI:10.1016/j.fgb.2008.09.001

Sequence

[ncr:NCU00552]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.99.30

IUBMB Enzyme Nomenclature:

1.3.99.30

ExPASy - ENZYME nomenclature database:

1.3.99.30

BRENDA, the Enzyme Database:

1.3.99.30

DBGET integrated database retrieval system