KEGG ENZYME: 1.4.2.3

ENZYME: 1.4.2.3

Entry

EC 1.4.2.3                  Enzyme

Name

pseudooxynicotine dehydrogenase;
pnaO (gene name)

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a cytochrome as acceptor

Sysname

4-(methylamino)-1-(pyridin-3-yl)butan-1-one:c-type cytochrome oxidoreductase (methylamine releasing)

Reaction(IUBMB)

pseudooxynicotine + H2O + 2 ferricytochrome c = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c + 2 H+ [RN:R10103]

Reaction(KEGG)

R10103

Substrate

pseudooxynicotine [CPD:C20361];
H2O [CPD:C00001];
ferricytochrome c [CPD:C00125]

Product

4-oxo-4-(pyridin-3-yl)butanal [CPD:C19567];
methylamine [CPD:C00218];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080]

Comment

Contains one non-covalently bound FAD molecule per dimer. This enzyme, characterized from the soil bacteria Pseudomonas sp. HZN6 and Pseudomonas putida S16, is involved in nicotine degradation.

History

EC 1.4.2.3 created 2012 as EC 1.4.3.24, transferred 2022 to EC 1.4.2.3

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K20158

pseudooxynicotine dehydrogenase

Genes

PPT:

PPS_4080

PMON:

X969_19990

PMOT:

X970_19625

PMED:

E3Z27_20800

PVK:	EPZ47_21950

PSJY:

AA098_03980 AA098_04095 AA098_21420

CUK:	KB879_35300

PPAI:

E1956_19245

Reference

1 [PMID:22267672]

Authors

Qiu J, Ma Y, Wen Y, Chen L, Wu L, Liu W

Title

Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine.

Journal

Appl Environ Microbiol 78:2154-60 (2012)
DOI:10.1128/AEM.07025-11

Sequence

[ag:AFD54463]

Reference

2 [PMID:35835223]

Authors

Choudhary V, Wu K, Zhang Z, Dulchavsky M, Barkman T, Bardwell JCA, Stull F.

Title

The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase.

Journal

J Biol Chem 298:102251 (2022)
DOI:10.1016/j.jbc.2022.102251

Sequence

[ppt:PPS_4080]

Other DBs

ExplorEnz - The Enzyme Database:

1.4.2.3

IUBMB Enzyme Nomenclature:

1.4.2.3

ExPASy - ENZYME nomenclature database:

1.4.2.3

BRENDA, the Enzyme Database:

1.4.2.3

DBGET integrated database retrieval system