KEGG ENZYME: 1.4.99.5

ENZYME: 1.4.99.5

Entry

EC 1.4.99.5                 Enzyme

Name

glycine dehydrogenase (cyanide-forming);
hydrogen cyanide synthase;
HCN synthase

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With unknown physiological acceptors

Sysname

glycine:acceptor oxidoreductase (hydrogen-cyanide-forming)

Reaction(IUBMB)

glycine + 2 acceptor = hydrogen cyanide + CO2 + 2 reduced acceptor [RN:R05704]

Reaction(KEGG)

R05704 > R00374

Substrate

glycine [CPD:C00037];
acceptor [CPD:C00028]

Product

hydrogen cyanide [CPD:C01326];
CO2 [CPD:C00011];
reduced acceptor [CPD:C00030]

Comment

The enzyme from Pseudomonas sp. contains FAD. The enzyme is membrane-bound, and the 2-electron acceptor is a component of the respiratory chain. The enzyme can act with various artificial electron acceptors, including phenazine methosulfate.

History

EC 1.4.99.5 created 2002

Pathway

ec00460

Cyanoamino acid metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K10814

hydrogen cyanide synthase HcnA

K10815

hydrogen cyanide synthase HcnB

K10816

hydrogen cyanide synthase HcnC

Genes

VCY:	IX92_17720 IX92_17725 IX92_17730

VCT:	JV59_21340 JV59_21345 JV59_21350

VAQ:	FIV01_19435(hcnC)

VNP:	KW548_21885 KW548_21890 KW548_21895

SALY:

E8E00_15310 E8E00_15315 E8E00_15320

SCOT:

HBA18_15205 HBA18_15210 HBA18_15215

SPRO:

N7E60_17180 N7E60_17185 N7E60_17190

PAE:	PA2193(hcnA) PA2194(hcnB) PA2195(hcnC)

PAEV:

N297_2266 N297_2267 N297_2268

PAEI:

N296_2266 N296_2267 N296_2268

PAU:	PA14_36310(hcnC) PA14_36320(hcnB) PA14_36330(hcnA)

PAP:	PSPA7_3101(hcnC) PSPA7_3102(hcnB) PSPA7_3103(hcnA)

PAG:	PLES_31301(hcnC) PLES_31311(hcnB) PLES_31321(hcnA)

PAF:	PAM18_2840(hcnC) PAM18_2841(hcnB) PAM18_2842(hcnA)

PNC:	NCGM2_3175(hcnA) NCGM2_3176(hcnB) NCGM2_3177(hcnC)

PDK:	PADK2_14680 PADK2_14685 PADK2_14690

PSG:	G655_14175 G655_14180 G655_14185

PRP:	M062_11375 M062_11380 M062_11385

PAEP:

PA1S_14755 PA1S_14760 PA1S_14765

PAER:

PA1R_gp5664 PA1R_gp5665 PA1R_gp5666

PAEM:

U769_14355 U769_14360 U769_14365

PAEL:

T223_15985 T223_15990 T223_15995

PAES:

SCV20265_3179 SCV20265_3180 SCV20265_3181

PAEU:

BN889_02406(hcnA) BN889_02407(hcnB) BN889_02408(hcnC)

PAEG:

AI22_19020 AI22_19025 AI22_19030

PAEC:

M802_2263 M802_2264 M802_2265

PAEO:

M801_2265 M801_2266 M801_2267

PPAA:

B7D75_14070 B7D75_14075 B7D75_14080

PMOL:

CLJ08_13065 CLJ08_13070 CLJ08_13075

PMOS:

O165_023000 O165_023005 O165_023010

PFL:	PFL_2577(hcnA) PFL_2578(hcnB) PFL_2579(hcnC)

PPRC:

PFLCHA0_c26420(hcnA) PFLCHA0_c26430(ooxA) PFLCHA0_c26440

PPRO:

PPC_2622(hcnA) PPC_2623(hcnB) PPC_2624(hcnC)

PFE:	PSF113_2367(hcnA) PSF113_2368(hcnB) PSF113_2369(hcnC)

PFO:	Pfl01_3514(hcnC) Pfl01_3515(hcnB) Pfl01_3516(hcnA)

PMAN:

OU5_6068 OU5_6069 OU5_6070

PCG:	AXG94_04380 AXG94_04385 AXG94_04390

PMED:

E3Z27_16665(hcnC) E3Z27_16670(hcnB) E3Z27_16675(hcnA)

PKR:	AYO71_19305 AYO71_19310 AYO71_19315

PEN:	PSEEN5520(hcnC) PSEEN5521(hcnB) PSEEN5522(hcnA)

PBA:	PSEBR_a3359 PSEBR_a3360 PSEBR_a3361

PBC:	CD58_12305 CD58_12310 CD58_12315

PCH:	EY04_11740 EY04_11745 EY04_11750

PCZ:	PCL1606_36260 PCL1606_36270 PCL1606_36280

PCP:	JM49_18105 JM49_18110 JM49_18115

PCHP:

C4K32_2519 C4K32_2520 C4K32_2521

PFZ:	AV641_13585 AV641_13590 AV641_13595

PLQ:	AA042_19290 AA042_19295 AA042_19300

PALK:

PSAKL28_52480 PSAKL28_52490 PSAKL28_52500

PSW:	LK03_05925 LK03_05930

PPV:	NJ69_19230 NJ69_19235 NJ69_19240

PSEM:

TO66_12560 TO66_12565 TO66_12570

PSEC:

CCOS191_5263(hcnC) CCOS191_5264(hcnB) CCOS191_5265(hcnA)

PPSY:

AOC04_10125 AOC04_10130 AOC04_10135

PSOS:

POS17_2580(hcnA) POS17_2581(hcnB) POS17_2582(hcnC)

PKE:	DLD99_17555 DLD99_17560 DLD99_17565

PGY:	AWU82_10070 AWU82_10075 AWU82_10080

PVK:	EPZ47_12195(hcnA) EPZ47_12200(hcnB) EPZ47_12205(hcnC)

PBZ:	GN234_05950(hcnC) GN234_05955(hcnB) GN234_05960(hcnA)

PATA:

JWU58_10955 JWU58_10960(hcnB) JWU58_10965(hcnC)

PDW:	BV82_2272(hcnC) BV82_2273(hcnB) BV82_2274(hcnA)

PZE:	HU754_011890(hcnA) HU754_011895(hcnB) HU754_011900(hcnC)

PMOE:

HV782_018050(hcnC) HV782_018055(hcnB) HV782_018060

PSEP:

C4K39_2594 C4K39_2595 C4K39_2596

PPII:

QL104_13580(hcnA) QL104_13585(hcnB) QL104_13590(hcnC)

PVW:	HU752_010780(hcnA) HU752_010785(hcnB) HU752_010790(hcnC)

PXA:	KSS93_02125(hcnA) KSS93_02130(hcnB) KSS93_02135(hcnC)

PTZ:	HU718_018835(hcnC) HU718_018840(hcnB) HU718_018845(hcnA)

PQI:	KH389_26980(hcnC) KH389_26985(hcnB) KH389_26990(hcnA)

PXN:	HU772_024760(hcnC) HU772_024765(hcnB) HU772_024770(hcnA)

PHV:	HU739_001835 HU739_001840(hcnB) HU739_001845(hcnC)

PFAK:

KSS94_26940(hcnC) KSS94_26945(hcnB) KSS94_26950(hcnA)

PMUY:

KSS95_03225(hcnA) KSS95_03230(hcnB) KSS95_03235(hcnC)

PMAM:

KSS90_25305(hcnC) KSS90_25310(hcnB) KSS90_25315(hcnA)

PALV:

KSS97_11955(hcnA) KSS97_11960(hcnB) KSS97_11965(hcnC)

PORY:

EJA05_27955(hcnC) EJA05_27960(hcnB) EJA05_27965(hcnA)

PTK:	EXN22_01415(hcnC) EXN22_01420(hcnB) EXN22_01425(hcnA)

PZA:	HU749_018035(hcnC) HU749_018040(hcnB) HU749_018045(hcnA)

PIE:	HU724_017695(hcnC) HU724_017700(hcnB) HU724_017705

PWY:	HU734_001010(hcnA) HU734_001015(hcnB) HU734_001020(hcnC)

PGF:	J0G10_18790(hcnC) J0G10_18795(hcnB) J0G10_18800(hcnA)

PSII:

NF676_16570(hcnC) NF676_16575(hcnB) NF676_16580

PSIH:

LOY51_26750(hcnC) LOY51_26755(hcnB) LOY51_26760(hcnA)

PCAS:

LOY40_11445(hcnA) LOY40_11450(hcnB) LOY40_11455(hcnC)

PFIT:

KJY40_18150(hcnC) KJY40_18155(hcnB) KJY40_18160(hcnA)

PNB:	NK667_14425(hcnC) NK667_14430(hcnB) NK667_14435(hcnA)

PCUC:

PSH97_17330(hcnC) PSH97_17335(hcnB) PSH97_17340

PRHZ:

CRX69_04945 CRX69_04950 CRX69_04955

THAT:

H3N35_17380 H3N35_17385 H3N35_17390

TACT:

SG35_018375 SG35_018380 SG35_018385

TVD:	SG34_010900 SG34_010905 SG34_010910

PRR:	AT705_00060 AT705_00065 AT705_00070

PLZ:	S4054249_17860 S4054249_17865 S4054249_17870

PALN:

B0W48_15825 B0W48_15830 B0W48_15835

PPIS:

B1L02_03835 B1L02_03840

PSEN:

PNC201_13070(hcnC) PNC201_13075(hcnB) PNC201_13080(hcnA)

PVB:	J5X90_12455 J5X90_12460 J5X90_12465

PSAZ:

PA25_08410(hcnC) PA25_08420(hcnB) PA25_08430(hcnA)

PFLI:

CTT31_13375 CTT31_13380 CTT31_13385

PMAZ:

R5H13_13860 R5H13_13865 R5H13_13870

HAXI:

HAALTHF_45770n

CVI:	CV_1682(hcnC) CV_1683(hcnB) CV_1684(hcnA)

CVC:	BKX93_21640 BKX93_21645 BKX93_21650

CHRO:

CXB49_13965 CXB49_13970 CXB49_13975

CHRI:

DK842_15670 DK842_15675 DK842_15680

CHRB:

DK843_21200 DK843_21205 DK843_21210

CRZ:	D1345_05980 D1345_05985 D1345_05990

CHAE:

CH06BL_12990(hcnC) CH06BL_13000(hcnB) CH06BL_13010(hcnA)

SECH:

B18_22880 B18_22885 B18_22890

STEL:

STAQ_37370(ooxA)

MSAG:

GCM10017556_23080(hcnC)

» show all

Reference

1 [PMID:234422]

Authors

Wissing F.

Title

Cyanide production from glycine by a homogenate from a Pseudomonas species.

Journal

J Bacteriol 121:695-9 (1975)
DOI:10.1128/JB.121.2.695-699.1975

Reference

2 [PMID:233722]

Authors

Castric PA

Title

Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.

Journal

J Bacteriol 130:826-31 (1977)
DOI:10.1128/JB.130.2.826-831.1977

Sequence

[pae:PA2193 PA2194 PA2195]

Reference

3 [PMID:9620970]

Authors

Laville J, Blumer C, Von Schroetter C, Gaia V, Defago G, Keel C, Haas D

Title

Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.

Journal

J Bacteriol 180:3187-96 (1998)
DOI:10.1128/JB.180.12.3187-3196.1998

Sequence

[pprc:PFLCHA0_c26420 PFLCHA0_c26430 PFLCHA0_c26440]

Reference

4 [PMID:10763748]

Authors

Blumer C, Haas D.

Title

Mechanism, regulation, and ecological role of bacterial cyanide biosynthesis.

Journal

Arch Microbiol 173:170-7 (2000)
DOI:10.1007/s002039900127