KEGG ENZYME: 1.5.3.19

ENZYME: 1.5.3.19

Entry

EC 1.5.3.19                 Enzyme

Name

4-methylaminobutanoate oxidase (formaldehyde-forming);
mabO (gene name)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor

Sysname

4-methylaminobutanoate:oxygen oxidoreductase (formaldehyde-forming)

Reaction(IUBMB)

4-methylaminobutanoate + O2 + H2O = 4-aminobutanoate + formaldehyde + H2O2 [RN:R10102]

Reaction(KEGG)

R10102

Substrate

4-methylaminobutanoate;
O2 [CPD:C00007];
H2O [CPD:C00001]

Product

4-aminobutanoate [CPD:C00334];
formaldehyde [CPD:C00067];
H2O2 [CPD:C00027]

Comment

A flavoprotein (FAD). In the enzyme from the soil bacterium Arthrobacter nicotinovorans the cofactor is covalently bound. Participates in the nicotine degradation pathway of this organism.

History

EC 1.5.3.19 created 2012

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K19191

4-methylaminobutanoate oxidase (formaldehyde-forming)

Genes

MHUM:

NNL39_01815

ARX:	ARZXY2_4583

PNV:	JMY29_20565

Reference

1 [PMID:15606755]

Authors

Chiribau CB, Sandu C, Fraaije M, Schiltz E, Brandsch R

Title

A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1.

Journal

Eur J Biochem 271:4677-84 (2004)
DOI:10.1111/j.1432-1033.2004.04432.x

Sequence

[ag:CAD47921]

Other DBs

ExplorEnz - The Enzyme Database:

1.5.3.19

IUBMB Enzyme Nomenclature:

1.5.3.19

ExPASy - ENZYME nomenclature database:

1.5.3.19

UM-BBD (Biocatalysis/Biodegradation Database):

1.5.3.19

BRENDA, the Enzyme Database:

1.5.3.19

DBGET integrated database retrieval system