KEGG ENZYME: 1.5.8.2

ENZYME: 1.5.8.2

Entry

EC 1.5.8.2                  Enzyme

Name

trimethylamine dehydrogenase

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With a flavin or flavoprotein as acceptor

Sysname

trimethylamine:electron-transfer flavoprotein oxidoreductase (demethylating)

Reaction(IUBMB)

trimethylamine + H2O + electron-transfer flavoprotein = dimethylamine + formaldehyde + reduced electron-transfer flavoprotein [RN:R02511]

Reaction(KEGG)

R02511

Substrate

trimethylamine [CPD:C00565];
H2O [CPD:C00001];
electron-transfer flavoprotein [CPD:C04253]

Product

dimethylamine [CPD:C00543];
formaldehyde [CPD:C00067];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

A number of alkyl-substituted derivatives of trimethylamine can also act as electron donors; phenazine methosulfate and 2,6-dichloroindophenol can act as electron acceptors. Contains FAD and a [4Fe-4S] cluster.

History

EC 1.5.8.2 created 1976 as EC 1.5.99.7, transferred 2002 to EC 1.5.8.2

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K00317

dimethylamine/trimethylamine dehydrogenase

Genes

PMEX:

H4W19_11290

RHD:	R2APBS1_0788

RTH:	LRK53_05475

DJI:	CH75_07125

DTX:	ATSB10_15590

XBA:	C7S18_14515

PRE:	PCA10_47330

PFC:	PflA506_4693(tmd)

POI:	BOP93_22725

PSHH:

HU773_024440

PHYG:

JTY93_23165

PTRL:

OU419_05970

HPIS:

P1P91_05060

TSN:	W908_00015

THIN:

CRN91_01375

RSO:

RSp0682

RSE:

F504_4539

RPU:	CDC45_21030

RNC:	GO999_17495

RSL:	RPSI07_mp0583

RSN:	RSPO_m01017(stcD)

RSM:	CMR15_mp10652

RSY:	RSUY_38890(tmd)

RSG:	JK151_04710

COX:	E0W60_31885

BPH:

Bphy_5960

BCAI:

K788_0001542

PHS:	C2L64_21885

PTER:

C2L65_26085

PSAA:

QEN71_34155

PPAI:

E1956_20380

PSPU:

NA29_08505

CDN:	BN940_13011

RHOA:

HZ993_23385

IDC:	LRM40_18490

CFU:

CFU_2684

CPRA:

CPter91_1920(tmd)

MEH:

M301_1400

MEI:	Msip34_2459

MEP:

MPQ_2403

AZA:	AZKH_p0217

AZI:	AzCIB_2375

MCI:	Mesci_2636

MCIC:

A4R28_14440

MOP:	Mesop_5984

MAM:	Mesau_02601

MESW:

A9K65_014455

MESP:

C1M53_02585

MERD:

EB233_12920 EB233_26910

MESR:

FGU64_14490

MMEI:

LRP31_28040

AAK:	AA2016_5993

PHT:	BLM14_27655

KMN:	HW532_16390

SME:

SMa0257

SMK:	Sinme_5357

SMX:	SM11_pC1602

SMI:	BN406_04939(tmd)

SMEG:

C770_GR4pC1294

SMEL:

SM2011_a0257

SMER:

DU99_24725

SMD:

Smed_1285

SIX:	BSY16_2386(hdh) BSY16_277

SKM:	PZL22_005785

ATA:	AWN88_01220

RHID:

FFM81_027465

SHZ:	shn_10745 shn_30205

SZO:	K8M09_09840 K8M09_20940

OIN:	IAR37_22770

OAN:

Oant_3262

OAH:	DR92_3227(hdh)

OPS:	A8A54_15775

BPSN:

NIK97_15890

OCH:	CES85_4558(tmd)

OCR:	HGK82_18870

OCL:	GTN27_18995

XAU:

Xaut_0917

STAR:

G3545_26390

LNE:	FZC33_09600 FZC33_21465

HDN:	Hden_0194 Hden_0535

HDT:	HYPDE_25228 HYPDE_25748 HYPDE_34963

HMC:	HYPMC_3962 HYPMC_3963

MCG:

GL4_2663

BLAG:

BLTE_16710

PLEO:

OHA_1_00468(tmd_1) OHA_1_00478(tmd_2)

NOH:	G5V57_02665 G5V57_05590 G5V57_06430 G5V57_31675

CAUL:

KCG34_05695 KCG34_22905

RUA:	D1823_18085

RCON:

K3740_03325

RDE:	RD1_4141(tmd)

RLI:	RLO149_c003340(tmd)

RPON:

G3256_12605 G3256_12610 G3256_12615

OAT:	OAN307_c42970(tmd)

OAR:	OA238_c47830(tmd1) OA238_c47840(tmd2) OA238_c47850(tmd3)

OTM:	OSB_15670(tmd)

LMD:	METH_21805

LEJ:	ETW24_01555 ETW24_05525

LCAE:

K3721_20240 K3721_20805

YAN:	AYJ57_15570

SUAM:

BOO69_07220

SULI:

C1J05_06740 C1J05_19580

SINL:

DSM14862_03441(tmd_1) DSM14862_03666(tmd_2)

RMM:	ROSMUCSMR3_03636(tmd)

ROK:	RAK1035_2462

RID:	RIdsm_00662(tmd_1) RIdsm_04243(tmd_2) RIdsm_04244(tmd_3)

ROM:	EI983_01485 EI983_01490 EI983_01495 EI983_09835 EI983_12585

ROH:	FIU89_18495(tmd)

RPEL:

N7U68_06485 N7U68_11895

AHT:	ANTHELSMS3_00564

THAA:

CFI11_03135

MALU:

KU6B_04850

RHP:	LPB142_07370

RBL:	B6K69_00080

PAMN:

pAMV3p0110(dmd) pAMV3p0194(tmd)

PSEW:

JHW44_16105

PSAP:

JHX88_10600

PFIS:

JHX87_13990

DON:	BSK21_05955

RBZ:	B9057_07560

GEH:	HYN69_03530 HYN69_12980

GFU:	KM031_01850 KM031_19465

TAW:	EI545_11145 EI545_19315

SEDI:

EBB79_22970 EBB79_23890

PPSO:

QPJ95_18105 QPJ95_18110 QPJ95_19445

HML:	HmaOT1_18870

PPRU:

FDP22_03105

PAED:

G5B38_12110 G5B38_17400 G5B38_20470

NSM:	JO391_00855 JO391_09270 JO391_14010

AALK:

LGT41_0005560

AMYL:

QBD29_02340 QBD29_12395

SNJ:	A7E77_04570

SMY:	BJP26_14925

SCH:	Sphch_1857

SLAA:

EUU25_06940 EUU25_06985(ndpC)

SMIC:

SmB9_20160

HTQ:	FRZ44_06770 FRZ44_10820 FRZ44_23470 FRZ44_47380

HADH:

FRZ61_06860 FRZ61_11050 FRZ61_23740

GSO:	PH603_02775

MDX:	BTO20_01230

MVQ:

MYVA_5919

MMAT:

MMAGJ_23260

MSEI:

MSEDJ_15750

MGO:	AFA91_30545

MRF:	MJO55_07995

MDF:	K0O62_08600

NYA:	LTV02_09175

RHA:	RHA1_ro01789

ROP:	ROP_14630 ROP_27330

ROA:	Pd630_LPD05948 Pd630_LPD07456

RKO:	JWS14_09075

RPSK:

JWS13_36935

GOI:	LK459_16700

GMG:	NWF22_14920

TPR:

Tpau_0381

SPHV:

F9278_41870

SANU:

K7396_16130

LDN:	H9L06_06465

NCA:	Noca_0579 Noca_0618

NARO:

CFH99_14995

PSIM:

KR76_16005

AGRA:

AGRA3207_001949

PHH:	AFB00_19150

KUT:	JJ691_53080(tmd)

PLK:	CIK06_05545

SBAE:

DSM104329_00584(tmd_1) DSM104329_02421(tmd_2) DSM104329_02427(tmd_3) DSM104329_03741(tmd_4)

RRS:	RoseRS_2964

RCA:

Rcas_1934

» show all

Reference

1 [PMID:5116569]

Authors

Colby J, Zatman LJ.

Title

The purification and properties of a bacterial trimethylamine dehydrogenase.

Journal

Biochem J 121:9P-10P (1971)
DOI:10.1042/bj1210009p

Reference

2 [PMID:204297]

Authors

Steenkamp DJ, Singer TP.

Title

Participation of the iron-sulphur cluster and of the covalently bound coenzyme of trimethylamine dehydrogenase in catalysis.

Journal

Biochem J 169:361-9 (1978)
DOI:10.1042/bj1690361

Reference

3 [PMID:7592591]

Authors

Huang L, Rohlfs RJ, Hille R.

Title

The reaction of trimethylamine dehydrogenase with electron transferring flavoprotein.

Journal

J Biol Chem 270:23958-65 (1995)
DOI:10.1074/jbc.270.41.23958

Reference

4 [PMID:11756429]

Authors

Jones M, Talfournier F, Bobrov A, Grossmann JG, Vekshin N, Sutcliffe MJ, Scrutton NS

Title

Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.

Journal

J Biol Chem 277:8457-65 (2002)
DOI:10.1074/jbc.M111105200

Reference

5 [PMID:11192721]

Authors

Scrutton NS, Sutcliffe MJ.

Title

Trimethylamine dehydrogenase and electron transferring flavoprotein.

Journal

Subcell Biochem 35:145-81 (2000)
DOI:10.1007/0-306-46828-x_5

Other DBs

ExplorEnz - The Enzyme Database:

1.5.8.2

IUBMB Enzyme Nomenclature:

1.5.8.2

ExPASy - ENZYME nomenclature database:

1.5.8.2

UM-BBD (Biocatalysis/Biodegradation Database):

1.5.8.2

BRENDA, the Enzyme Database:

1.5.8.2

CAS:	39307-09-0

DBGET integrated database retrieval system