KEGG   ENZYME: 1.5.99.14
Entry
EC 1.5.99.14                Enzyme                                 
Name
6-hydroxypseudooxynicotine dehydrogenase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With unknown physiological acceptors
Sysname
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating)
Reaction(IUBMB)
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O = 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor [RN:R07514]
Reaction(KEGG)
R07514
Substrate
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C01297];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Product
1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C15986];
reduced acceptor [CPD:C00030]
Comment
Contains a cytidylyl molybdenum cofactor [3]. The enzyme, which participates in the nicotine degradation pathway, has been characterized from the soil bacterium Arthrobacter nicotinovorans [1,2].
History
EC 1.5.99.14 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K19185  6-hydroxypseudooxynicotine dehydrogenase subunit alpha
K19186  6-hydroxypseudooxynicotine dehydrogenase subunit beta
K19187  6-hydroxypseudooxynicotine dehydrogenase subunit gamma
Genes
PDIOPDMSB3_0230
BGVCAL12_03285
AMISAmn_pb01250
ANJAMD1_0530(kdhA)
BCOUIC761_22645
RBKE0H22_06170
MOGMMB17_04665
ACUTMRB58_00995
BACAFAY30_04545 FAY30_22140 FAY30_22150
CFIRNAF01_14280
SCIAHUG15_03260
ROPROP_27460(kdhL) ROP_27510(kdhM) ROP_27520(kdhB)
ROAPd630_LPD07471 Pd630_LPD07476 Pd630_LPD07477
RGORNMQ04_20060
GMGNWF22_15110
SGRFSGFS_017820 SGFS_017990
SCOAQU709_39080 QU709_39160
ARXARZXY2_4556 ARZXY2_4561
PNVJMY29_20645 JMY29_20670 JMY29_20675
TEDU5C87_22510 U5C87_22555 U5C87_22575 U5C87_22580
NCANoca_0606 Noca_0607 Noca_0612
SGRGL0C25_00100 L0C25_09460 L0C25_23800
SAIUJ4H86_13095
ACTQOG417_48805
MHAIOHB01_11540
BLAPMVA48_02370
AACDLWP59_13125 LWP59_23005
ARHDVSH64_14635
ACTYOG774_11410 OG774_13220
AIHAiant_73530(cutM)
ACTLL3i22_029960(cutM)
ASICQ0Z83_017340
BALADSM104299_03660(kdhA)
SBAEDSM104329_02309(kdhA_1) DSM104329_05423(kdhA_6)
UAMUABAM_05846
 » show all
Reference
1
  Authors
Freudenberg, W., Konig, K. and Andreesen, J. R.
  Title
Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase.
  Journal
FEMS Microbiology Letters 52:13-18 (1988)
Reference
2  [PMID:7815950]
  Authors
Grether-Beck S, Igloi GL, Pust S, Schilz E, Decker K, Brandsch R
  Title
Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans.
  Journal
Mol Microbiol 13:929-36 (1994)
DOI:10.1111/j.1365-2958.1994.tb00484.x
Reference
3  [PMID:16820521]
  Authors
Sachelaru P, Schiltz E, Brandsch R
  Title
A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans.
  Journal
Appl Environ Microbiol 72:5126-31 (2006)
DOI:10.1128/AEM.00437-06
Other DBs
ExplorEnz - The Enzyme Database: 1.5.99.14
IUBMB Enzyme Nomenclature: 1.5.99.14
ExPASy - ENZYME nomenclature database: 1.5.99.14
UM-BBD (Biocatalysis/Biodegradation Database): 1.5.99.14
BRENDA, the Enzyme Database: 1.5.99.14

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